Positive Selection Vectors to Generate Fused Genes for the Expression of His-Tagged Proteins
Epitope tagging simplifies detection, characterization and purification of proteins. Gene fusion to combine the coding region of a well-characterized epitope with the coding region for a protein of interest generally requires several subcloning steps. Alternatively, a PCR strategy can be used to gen...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-11-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98255pf01 |
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