Transcriptomic and Functional Insights of Calcium Suppresses Grape Berry Cracking: Antioxidant Activation, ABA Pathway Modulation, and VITPL1-Mediated Cell Wall Stability
Calcium treatments are effective in reducing grapevine berry cracking. However, the underlying mechanism of calcium on grapevine berry cracking is not well-known. This work aims to explore the potential molecular mechanism of calcium treatment regulating grapevine berry cracking. 5 g/L of calcium ch...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | International Journal of Fruit Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15538362.2025.2498028 |
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| author | Shi Hao Zhou Wen Hua He Xiao e Xu Yinyu Wang Yun |
| author_facet | Shi Hao Zhou Wen Hua He Xiao e Xu Yinyu Wang Yun |
| author_sort | Shi Hao |
| collection | DOAJ |
| description | Calcium treatments are effective in reducing grapevine berry cracking. However, the underlying mechanism of calcium on grapevine berry cracking is not well-known. This work aims to explore the potential molecular mechanism of calcium treatment regulating grapevine berry cracking. 5 g/L of calcium chloride was sprayed at flowering period (A), early (B) and late (C) fruit development period of grapevine, non-calcium sprayed treatment as control (D). The molecular mechanism of calcium treatment on berry skin cracking was studied by RNA-seq. Meanwhile, the key genes [pectin lyase gene (VITPL1)] were analyzed for function verification of overexpression. The other glycan degradation, Peroxisome, Oxidative phosphorylation, Plant hormone signal transduction, and Diterpenoid biosynthesis (p < .05) were directly related to fruit cracking. Meanwhile, 20 genes related to antioxidase were identified, treatment with calcium increased the expression of genes associated with the antioxidant enzyme. Transcripts related to the xanthine dehydrogenase and abscisic aldehyde oxidase (ABA pathway) were found to be down-regulated. 70 genes related to cell wall catabolism were identified, treatment with calcium decreased the expression of genes associated with cell wall catabolism. Furthermore, the VITPL1 gene has a strong effect on grapevine fruit cracking. Calcium treatment significantly reduced the expression level of VITPL1. Calcium can reduce fruit cracking by increasing the level of antioxidant enzyme genes, and decreasing the level of ABA synthesis genes and cell wall catabolism genes (in particular, VITPL1) in the pericarp. Meanwhile, overall A treatment group was the more effective. |
| format | Article |
| id | doaj-art-b494f07a03174e5ea8a9130580e7568b |
| institution | OA Journals |
| issn | 1553-8362 1553-8621 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
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| series | International Journal of Fruit Science |
| spelling | doaj-art-b494f07a03174e5ea8a9130580e7568b2025-08-20T02:11:08ZengTaylor & Francis GroupInternational Journal of Fruit Science1553-83621553-86212025-12-0125116017710.1080/15538362.2025.2498028Transcriptomic and Functional Insights of Calcium Suppresses Grape Berry Cracking: Antioxidant Activation, ABA Pathway Modulation, and VITPL1-Mediated Cell Wall StabilityShi Hao0Zhou Wen Hua1He Xiao e2Xu Yinyu3Wang Yun4College of Life and Environmental Science, Hunan University of Arts and Science, Changde, ChinaCollege of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, ChinaCollege of Agriculture and Forestry Science, Hunan Applied Technology University, Changde, ChinaCollege of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, ChinaCollege of Life and Environmental Science, Hunan University of Arts and Science, Changde, ChinaCalcium treatments are effective in reducing grapevine berry cracking. However, the underlying mechanism of calcium on grapevine berry cracking is not well-known. This work aims to explore the potential molecular mechanism of calcium treatment regulating grapevine berry cracking. 5 g/L of calcium chloride was sprayed at flowering period (A), early (B) and late (C) fruit development period of grapevine, non-calcium sprayed treatment as control (D). The molecular mechanism of calcium treatment on berry skin cracking was studied by RNA-seq. Meanwhile, the key genes [pectin lyase gene (VITPL1)] were analyzed for function verification of overexpression. The other glycan degradation, Peroxisome, Oxidative phosphorylation, Plant hormone signal transduction, and Diterpenoid biosynthesis (p < .05) were directly related to fruit cracking. Meanwhile, 20 genes related to antioxidase were identified, treatment with calcium increased the expression of genes associated with the antioxidant enzyme. Transcripts related to the xanthine dehydrogenase and abscisic aldehyde oxidase (ABA pathway) were found to be down-regulated. 70 genes related to cell wall catabolism were identified, treatment with calcium decreased the expression of genes associated with cell wall catabolism. Furthermore, the VITPL1 gene has a strong effect on grapevine fruit cracking. Calcium treatment significantly reduced the expression level of VITPL1. Calcium can reduce fruit cracking by increasing the level of antioxidant enzyme genes, and decreasing the level of ABA synthesis genes and cell wall catabolism genes (in particular, VITPL1) in the pericarp. Meanwhile, overall A treatment group was the more effective.https://www.tandfonline.com/doi/10.1080/15538362.2025.2498028Calciumabscisic acid signalingcell wall modificationantioxidasegrapevinefruit cracking |
| spellingShingle | Shi Hao Zhou Wen Hua He Xiao e Xu Yinyu Wang Yun Transcriptomic and Functional Insights of Calcium Suppresses Grape Berry Cracking: Antioxidant Activation, ABA Pathway Modulation, and VITPL1-Mediated Cell Wall Stability International Journal of Fruit Science Calcium abscisic acid signaling cell wall modification antioxidase grapevine fruit cracking |
| title | Transcriptomic and Functional Insights of Calcium Suppresses Grape Berry Cracking: Antioxidant Activation, ABA Pathway Modulation, and VITPL1-Mediated Cell Wall Stability |
| title_full | Transcriptomic and Functional Insights of Calcium Suppresses Grape Berry Cracking: Antioxidant Activation, ABA Pathway Modulation, and VITPL1-Mediated Cell Wall Stability |
| title_fullStr | Transcriptomic and Functional Insights of Calcium Suppresses Grape Berry Cracking: Antioxidant Activation, ABA Pathway Modulation, and VITPL1-Mediated Cell Wall Stability |
| title_full_unstemmed | Transcriptomic and Functional Insights of Calcium Suppresses Grape Berry Cracking: Antioxidant Activation, ABA Pathway Modulation, and VITPL1-Mediated Cell Wall Stability |
| title_short | Transcriptomic and Functional Insights of Calcium Suppresses Grape Berry Cracking: Antioxidant Activation, ABA Pathway Modulation, and VITPL1-Mediated Cell Wall Stability |
| title_sort | transcriptomic and functional insights of calcium suppresses grape berry cracking antioxidant activation aba pathway modulation and vitpl1 mediated cell wall stability |
| topic | Calcium abscisic acid signaling cell wall modification antioxidase grapevine fruit cracking |
| url | https://www.tandfonline.com/doi/10.1080/15538362.2025.2498028 |
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