Antibacterial activity of 5-bromo substituted phenyl N-acylhydrazone derivatives with aromatic substitution at ortho- and para- directors as potential adjuvants
Antibiotics are frequently used in the food industry to ensure food safety and sustainability, predominantly in treating bacterial infections in food animals. Due to the massive use of antibiotics, emergence of antibiotic resistance (ABR) and multidrug resistance (MDR) threaten public health. Antibi...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/33/bioconf_icfsb2025_02008.pdf |
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| _version_ | 1849421098845732864 |
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| author | Lim Jin Yu Sim Kooi Mow Teo Kah Cheng |
| author_facet | Lim Jin Yu Sim Kooi Mow Teo Kah Cheng |
| author_sort | Lim Jin Yu |
| collection | DOAJ |
| description | Antibiotics are frequently used in the food industry to ensure food safety and sustainability, predominantly in treating bacterial infections in food animals. Due to the massive use of antibiotics, emergence of antibiotic resistance (ABR) and multidrug resistance (MDR) threaten public health. Antibiotic adjuvants serve as an alternative strategy to combat these issues. N-acylhydrazone (NAH) has been proven to be a promising and versatile motif in drug design and medicinal chemistry due to its pronounced antimicrobial effect. Nine chemically synthesised NAH derivatives were tested for in vitro antibacterial activity against selected Gram-positive, Gram-negative and resistant strains using broth microdilution assay to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The study showed that Compound 9 exhibited the highest antibacterial activity against Bacillus subtilis and Pseudomonas aeruginosa (MIC and MBC = 62.50 µg/mL) among the nine tested compounds. Furthermore, the interaction between tested compounds and streptomycin were evaluated through calculation of fractional inhibitory concentration (FIC) index. Combination of all tested compounds with streptomycin demonstrated strong to moderate inhibitory activity against all tested bacterial strains (MIC = 7.81–62.50 µg/mL), while Compounds 1 and 2 were potential adjuvants due to synergistic effect observed in combination with streptomycin against Staphylococcus aureus. |
| format | Article |
| id | doaj-art-b4900aaee7e24e3fa67bd242b55c9dba |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-b4900aaee7e24e3fa67bd242b55c9dba2025-08-20T03:31:33ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011820200810.1051/bioconf/202518202008bioconf_icfsb2025_02008Antibacterial activity of 5-bromo substituted phenyl N-acylhydrazone derivatives with aromatic substitution at ortho- and para- directors as potential adjuvantsLim Jin Yu0Sim Kooi Mow1Teo Kah Cheng2Faculty of Science, Department of Agricultural and Food Science, Universiti Tunku Abdul RahmanFaculty of Science, Department of Chemical Science, Universiti Tunku Abdul RahmanFaculty of Science, Department of Agricultural and Food Science, Universiti Tunku Abdul RahmanAntibiotics are frequently used in the food industry to ensure food safety and sustainability, predominantly in treating bacterial infections in food animals. Due to the massive use of antibiotics, emergence of antibiotic resistance (ABR) and multidrug resistance (MDR) threaten public health. Antibiotic adjuvants serve as an alternative strategy to combat these issues. N-acylhydrazone (NAH) has been proven to be a promising and versatile motif in drug design and medicinal chemistry due to its pronounced antimicrobial effect. Nine chemically synthesised NAH derivatives were tested for in vitro antibacterial activity against selected Gram-positive, Gram-negative and resistant strains using broth microdilution assay to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The study showed that Compound 9 exhibited the highest antibacterial activity against Bacillus subtilis and Pseudomonas aeruginosa (MIC and MBC = 62.50 µg/mL) among the nine tested compounds. Furthermore, the interaction between tested compounds and streptomycin were evaluated through calculation of fractional inhibitory concentration (FIC) index. Combination of all tested compounds with streptomycin demonstrated strong to moderate inhibitory activity against all tested bacterial strains (MIC = 7.81–62.50 µg/mL), while Compounds 1 and 2 were potential adjuvants due to synergistic effect observed in combination with streptomycin against Staphylococcus aureus.https://www.bio-conferences.org/articles/bioconf/pdf/2025/33/bioconf_icfsb2025_02008.pdf |
| spellingShingle | Lim Jin Yu Sim Kooi Mow Teo Kah Cheng Antibacterial activity of 5-bromo substituted phenyl N-acylhydrazone derivatives with aromatic substitution at ortho- and para- directors as potential adjuvants BIO Web of Conferences |
| title | Antibacterial activity of 5-bromo substituted phenyl N-acylhydrazone derivatives with aromatic substitution at ortho- and para- directors as potential adjuvants |
| title_full | Antibacterial activity of 5-bromo substituted phenyl N-acylhydrazone derivatives with aromatic substitution at ortho- and para- directors as potential adjuvants |
| title_fullStr | Antibacterial activity of 5-bromo substituted phenyl N-acylhydrazone derivatives with aromatic substitution at ortho- and para- directors as potential adjuvants |
| title_full_unstemmed | Antibacterial activity of 5-bromo substituted phenyl N-acylhydrazone derivatives with aromatic substitution at ortho- and para- directors as potential adjuvants |
| title_short | Antibacterial activity of 5-bromo substituted phenyl N-acylhydrazone derivatives with aromatic substitution at ortho- and para- directors as potential adjuvants |
| title_sort | antibacterial activity of 5 bromo substituted phenyl n acylhydrazone derivatives with aromatic substitution at ortho and para directors as potential adjuvants |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/33/bioconf_icfsb2025_02008.pdf |
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