Designing Gold Nanoparticle-Ensembles as Surface Enhanced Raman Scattering Tags inside Human Retinal Cells
Apart from the traditional development of surface-enhanced raman scattering (SERS) substrates for ultrasensitive spectroscopic analysis, an increasing interest is given nowadays to the design of the so-called SERS nanotags which integrate multiple SERS applications into single plasmonic nanoparticle...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2012/961216 |
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| _version_ | 1841524887891476480 |
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| author | Sanda Boca Dumitrita Rugina Adela Pintea Nicolae Leopold Simion Astilean |
| author_facet | Sanda Boca Dumitrita Rugina Adela Pintea Nicolae Leopold Simion Astilean |
| author_sort | Sanda Boca |
| collection | DOAJ |
| description | Apart from the traditional development of surface-enhanced raman scattering (SERS) substrates for ultrasensitive spectroscopic analysis, an increasing interest is given nowadays to the design of the so-called SERS nanotags which integrate multiple SERS applications into single plasmonic nanoparticles. The fabrication of SERS tags is still a challenging task due to the complicated fabrication process. Typically, SERS tags are hybrid nanoconstructs consisting in a unique plasmonic nanoobject encoded with specific reporter molecules and enveloped in a protective shell that provides both biocompatibility and targeting function. Herein, we produce effective SERS tags consisting in small aggregates of gold nanoparticles (mainly dimers and trimers) which are captured from solution and then transferred into cells to perform as individual plasmonic nanostructures. Actually the small aggregates formed under controlled conditions are stabilized in solution by interlocking into a polymeric envelope made of thiol-modified poly(ethylene) glycol (PEG-SH). No further encoding operation is necessary in our case since part of ascorbic acid used as reducing agent remains attached in the interparticle junctions, providing persistent and strong SERS signal when the fabricated tags are internalized by human retinal cells. Our studies demonstrate a promising potential of new SERS-active nanoparticles to serve as effective reporters for biomedical tracing and imaging. |
| format | Article |
| id | doaj-art-b48df4332e704cff8b92ee65d65ce7ad |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-b48df4332e704cff8b92ee65d65ce7ad2025-02-03T05:47:09ZengWileyJournal of Nanotechnology1687-95031687-95112012-01-01201210.1155/2012/961216961216Designing Gold Nanoparticle-Ensembles as Surface Enhanced Raman Scattering Tags inside Human Retinal CellsSanda Boca0Dumitrita Rugina1Adela Pintea2Nicolae Leopold3Simion Astilean4Nanobiophotonics and Laser Microspectroscopy Center, Institute for Interdisciplinary Research in Bio-Nano-Sciences and Faculty of Physics, Babes-Bolyai University, Treboniu Laurian 42, 400271 Cluj-Napoca, RomaniaDepartment of Biochemistry, University of Agricultural Sciences and Veterinary Medicine, Manastur 3-5, 400372 Cluj-Napoca, RomaniaDepartment of Biochemistry, University of Agricultural Sciences and Veterinary Medicine, Manastur 3-5, 400372 Cluj-Napoca, RomaniaFaculty of Physics, Babes-Bolyai University, Kogalniceanu 1, 400084 Cluj-Napoca, RomaniaNanobiophotonics and Laser Microspectroscopy Center, Institute for Interdisciplinary Research in Bio-Nano-Sciences and Faculty of Physics, Babes-Bolyai University, Treboniu Laurian 42, 400271 Cluj-Napoca, RomaniaApart from the traditional development of surface-enhanced raman scattering (SERS) substrates for ultrasensitive spectroscopic analysis, an increasing interest is given nowadays to the design of the so-called SERS nanotags which integrate multiple SERS applications into single plasmonic nanoparticles. The fabrication of SERS tags is still a challenging task due to the complicated fabrication process. Typically, SERS tags are hybrid nanoconstructs consisting in a unique plasmonic nanoobject encoded with specific reporter molecules and enveloped in a protective shell that provides both biocompatibility and targeting function. Herein, we produce effective SERS tags consisting in small aggregates of gold nanoparticles (mainly dimers and trimers) which are captured from solution and then transferred into cells to perform as individual plasmonic nanostructures. Actually the small aggregates formed under controlled conditions are stabilized in solution by interlocking into a polymeric envelope made of thiol-modified poly(ethylene) glycol (PEG-SH). No further encoding operation is necessary in our case since part of ascorbic acid used as reducing agent remains attached in the interparticle junctions, providing persistent and strong SERS signal when the fabricated tags are internalized by human retinal cells. Our studies demonstrate a promising potential of new SERS-active nanoparticles to serve as effective reporters for biomedical tracing and imaging.http://dx.doi.org/10.1155/2012/961216 |
| spellingShingle | Sanda Boca Dumitrita Rugina Adela Pintea Nicolae Leopold Simion Astilean Designing Gold Nanoparticle-Ensembles as Surface Enhanced Raman Scattering Tags inside Human Retinal Cells Journal of Nanotechnology |
| title | Designing Gold Nanoparticle-Ensembles as Surface Enhanced Raman Scattering Tags inside Human Retinal Cells |
| title_full | Designing Gold Nanoparticle-Ensembles as Surface Enhanced Raman Scattering Tags inside Human Retinal Cells |
| title_fullStr | Designing Gold Nanoparticle-Ensembles as Surface Enhanced Raman Scattering Tags inside Human Retinal Cells |
| title_full_unstemmed | Designing Gold Nanoparticle-Ensembles as Surface Enhanced Raman Scattering Tags inside Human Retinal Cells |
| title_short | Designing Gold Nanoparticle-Ensembles as Surface Enhanced Raman Scattering Tags inside Human Retinal Cells |
| title_sort | designing gold nanoparticle ensembles as surface enhanced raman scattering tags inside human retinal cells |
| url | http://dx.doi.org/10.1155/2012/961216 |
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