Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insects
Abstract Trilocha varians is a member of the bombycid moths. Since T. varians has a considerably shorter generation period than the prevailing model species, Bombyx mori, this species would be a novel model insect in Lepidoptera. To facilitate further use of T. varians, we developed genome annotatio...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04411-3 |
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| author | Jung Lee Toshiaki Fujimoto Katsushi Yamaguchi Shuji Shigenobu Ken Sahara Toru Shimada |
| author_facet | Jung Lee Toshiaki Fujimoto Katsushi Yamaguchi Shuji Shigenobu Ken Sahara Toru Shimada |
| author_sort | Jung Lee |
| collection | DOAJ |
| description | Abstract Trilocha varians is a member of the bombycid moths. Since T. varians has a considerably shorter generation period than the prevailing model species, Bombyx mori, this species would be a novel model insect in Lepidoptera. To facilitate further use of T. varians, we developed genome annotation information on the chromosome-scale assembly of T. varians previously published by our group. 9 RNA-seq datasets and 2 Iso-seq datasets were submitted for transcriptome-based gene prediction. As a result, 16,266 protein-coding genes were predicted on the latest genome assembly, and 98.6% of BUSCO sequences were present in our gene models. ATAC-seq was also conducted to determine chromatin accessibility across the genome. Finally, piRNA-targeted small RNA-seq revealed T. varians genome harbours 517 piRNA clusters (piCs). This information will encourage and facilitate potential users who plan to use this species. |
| format | Article |
| id | doaj-art-b48ce0f2d25b469588fba6c4aac7e27e |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-b48ce0f2d25b469588fba6c4aac7e27e2025-01-26T12:14:46ZengNature PortfolioScientific Data2052-44632025-01-0112111010.1038/s41597-025-04411-3Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insectsJung Lee0Toshiaki Fujimoto1Katsushi Yamaguchi2Shuji Shigenobu3Ken Sahara4Toru Shimada5Gakushuin University, Faculty of Science, Department of Life ScienceLaboratory of Applied Entomology, Faculty of Agriculture, Iwate UniversityNational Institute for Basic Biology, Trans-Omics FacilityNational Institute for Basic Biology, Trans-Omics FacilityLaboratory of Applied Entomology, Faculty of Agriculture, Iwate UniversityGakushuin University, Faculty of Science, Department of Life ScienceAbstract Trilocha varians is a member of the bombycid moths. Since T. varians has a considerably shorter generation period than the prevailing model species, Bombyx mori, this species would be a novel model insect in Lepidoptera. To facilitate further use of T. varians, we developed genome annotation information on the chromosome-scale assembly of T. varians previously published by our group. 9 RNA-seq datasets and 2 Iso-seq datasets were submitted for transcriptome-based gene prediction. As a result, 16,266 protein-coding genes were predicted on the latest genome assembly, and 98.6% of BUSCO sequences were present in our gene models. ATAC-seq was also conducted to determine chromatin accessibility across the genome. Finally, piRNA-targeted small RNA-seq revealed T. varians genome harbours 517 piRNA clusters (piCs). This information will encourage and facilitate potential users who plan to use this species.https://doi.org/10.1038/s41597-025-04411-3 |
| spellingShingle | Jung Lee Toshiaki Fujimoto Katsushi Yamaguchi Shuji Shigenobu Ken Sahara Toru Shimada Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insects Scientific Data |
| title | Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insects |
| title_full | Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insects |
| title_fullStr | Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insects |
| title_full_unstemmed | Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insects |
| title_short | Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insects |
| title_sort | comprehensive genome annotation of trilocha varians a new model species of lepidopteran insects |
| url | https://doi.org/10.1038/s41597-025-04411-3 |
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