Microencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study.
There is a growing trend for researchers to use in vitro 3D models in cancer studies, as they can better recapitulate the complex in vivo situation. And the fact that the progression and development of tumor are closely associated to its stromal microenvironment has been increasingly recognized. The...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144139&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850189079158718464 |
|---|---|
| author | Pan Yeung Hoi Shun Sin Shing Chan Godfrey Chi Fung Chan Barbara Pui Chan |
| author_facet | Pan Yeung Hoi Shun Sin Shing Chan Godfrey Chi Fung Chan Barbara Pui Chan |
| author_sort | Pan Yeung |
| collection | DOAJ |
| description | There is a growing trend for researchers to use in vitro 3D models in cancer studies, as they can better recapitulate the complex in vivo situation. And the fact that the progression and development of tumor are closely associated to its stromal microenvironment has been increasingly recognized. The establishment of such tumor supportive niche is vital in understanding tumor progress and metastasis. The mesenchymal origin of many cells residing in the cancer niche provides the rationale to include MSCs in mimicking the niche in neuroblastoma. Here we co-encapsulate and co-culture NBCs and MSCs in a 3D in vitro model and investigate the morphology, growth kinetics and matrix remodeling in the reconstituted stromal environment. Results showed that the incorporation of MSCs in the model lead to accelerated growth of cancer cells as well as recapitulation of at least partially the tumor microenvironment in vivo. The current study therefore demonstrates the feasibility for the collagen microsphere to act as a 3D in vitro cancer model for various topics in cancer studies. |
| format | Article |
| id | doaj-art-b48c1bce3e1a493a845076d09ede6cf8 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b48c1bce3e1a493a845076d09ede6cf82025-08-20T02:15:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014413910.1371/journal.pone.0144139Microencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study.Pan YeungHoi Shun SinShing ChanGodfrey Chi Fung ChanBarbara Pui ChanThere is a growing trend for researchers to use in vitro 3D models in cancer studies, as they can better recapitulate the complex in vivo situation. And the fact that the progression and development of tumor are closely associated to its stromal microenvironment has been increasingly recognized. The establishment of such tumor supportive niche is vital in understanding tumor progress and metastasis. The mesenchymal origin of many cells residing in the cancer niche provides the rationale to include MSCs in mimicking the niche in neuroblastoma. Here we co-encapsulate and co-culture NBCs and MSCs in a 3D in vitro model and investigate the morphology, growth kinetics and matrix remodeling in the reconstituted stromal environment. Results showed that the incorporation of MSCs in the model lead to accelerated growth of cancer cells as well as recapitulation of at least partially the tumor microenvironment in vivo. The current study therefore demonstrates the feasibility for the collagen microsphere to act as a 3D in vitro cancer model for various topics in cancer studies.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144139&type=printable |
| spellingShingle | Pan Yeung Hoi Shun Sin Shing Chan Godfrey Chi Fung Chan Barbara Pui Chan Microencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study. PLoS ONE |
| title | Microencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study. |
| title_full | Microencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study. |
| title_fullStr | Microencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study. |
| title_full_unstemmed | Microencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study. |
| title_short | Microencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study. |
| title_sort | microencapsulation of neuroblastoma cells and mesenchymal stromal cells in collagen microspheres a 3d model for cancer cell niche study |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144139&type=printable |
| work_keys_str_mv | AT panyeung microencapsulationofneuroblastomacellsandmesenchymalstromalcellsincollagenmicrospheresa3dmodelforcancercellnichestudy AT hoishunsin microencapsulationofneuroblastomacellsandmesenchymalstromalcellsincollagenmicrospheresa3dmodelforcancercellnichestudy AT shingchan microencapsulationofneuroblastomacellsandmesenchymalstromalcellsincollagenmicrospheresa3dmodelforcancercellnichestudy AT godfreychifungchan microencapsulationofneuroblastomacellsandmesenchymalstromalcellsincollagenmicrospheresa3dmodelforcancercellnichestudy AT barbarapuichan microencapsulationofneuroblastomacellsandmesenchymalstromalcellsincollagenmicrospheresa3dmodelforcancercellnichestudy |