RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America.
Epizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assay...
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Public Library of Science (PLoS)
2010-09-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0012782&type=printable |
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| author | Narender S Maan Sushila Maan Kyriaki Nomikou Donna J Johnson Mehdi El Harrak Hafsa Madani Hagai Yadin Serife Incoglu Kadir Yesilbag Andrew B Allison David E Stallknecht Carrie Batten Simon J Anthony Peter P C Mertens |
| author_facet | Narender S Maan Sushila Maan Kyriaki Nomikou Donna J Johnson Mehdi El Harrak Hafsa Madani Hagai Yadin Serife Incoglu Kadir Yesilbag Andrew B Allison David E Stallknecht Carrie Batten Simon J Anthony Peter P C Mertens |
| author_sort | Narender S Maan |
| collection | DOAJ |
| description | Epizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assays. EHDV outer-capsid protein VP2 (encoded by genome-segment 2 [Seg-2]) is highly variable and represents the primary target for neutralising antibodies generated by the mammalian host. Consequently VP2 is also the primary determinant of virus "serotype", as identified in virus neutralisation tests (VNT). Although previous reports have indicated eight to ten EHDV serotypes, recent serological comparisons and molecular analyses of Seg-2 indicate only seven EHDV "types". Oligonucleotide primers were developed targeting Seg-2, for use in conventional RT-PCR assays to detect and identify these seven types. These assays, which are more rapid and sensitive, still show complete agreement with VNT and were used to identify recent EHDV isolates from the Mediterranean region and North America. |
| format | Article |
| id | doaj-art-b484d9d953ec494ba41d66467fc9cd55 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2010-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b484d9d953ec494ba41d66467fc9cd552025-08-20T03:07:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-09-0159e1278210.1371/journal.pone.0012782RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America.Narender S MaanSushila MaanKyriaki NomikouDonna J JohnsonMehdi El HarrakHafsa MadaniHagai YadinSerife IncogluKadir YesilbagAndrew B AllisonDavid E StallknechtCarrie BattenSimon J AnthonyPeter P C MertensEpizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assays. EHDV outer-capsid protein VP2 (encoded by genome-segment 2 [Seg-2]) is highly variable and represents the primary target for neutralising antibodies generated by the mammalian host. Consequently VP2 is also the primary determinant of virus "serotype", as identified in virus neutralisation tests (VNT). Although previous reports have indicated eight to ten EHDV serotypes, recent serological comparisons and molecular analyses of Seg-2 indicate only seven EHDV "types". Oligonucleotide primers were developed targeting Seg-2, for use in conventional RT-PCR assays to detect and identify these seven types. These assays, which are more rapid and sensitive, still show complete agreement with VNT and were used to identify recent EHDV isolates from the Mediterranean region and North America.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0012782&type=printable |
| spellingShingle | Narender S Maan Sushila Maan Kyriaki Nomikou Donna J Johnson Mehdi El Harrak Hafsa Madani Hagai Yadin Serife Incoglu Kadir Yesilbag Andrew B Allison David E Stallknecht Carrie Batten Simon J Anthony Peter P C Mertens RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America. PLoS ONE |
| title | RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America. |
| title_full | RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America. |
| title_fullStr | RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America. |
| title_full_unstemmed | RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America. |
| title_short | RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America. |
| title_sort | rt pcr assays for seven serotypes of epizootic haemorrhagic disease virus amp their use to type strains from the mediterranean region and north america |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0012782&type=printable |
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