Genetic variation analysis of EMS-induced Curcuma alismatifolia Gagnep. mutants using SSR markers
Abstract Curcuma alismatifolia Gagnep. is an original ornamental flower. To induce new phenotypic variations and breed new varieties with market value, ethyl methanesulfonate (EMS) was used to treat C. alismatifolia seedlings at various concentrations (0.0%, 0.1%, 0.5%, 1.0%, 1.5% and 2.0%) and for...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
CABI
2025-06-01
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| Series: | European Journal of Horticultural Science |
| Subjects: | |
| Online Access: | http://www.cabidigitallibrary.org/doi/10.1079/ejhs.2025.0011 |
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| Summary: | Abstract Curcuma alismatifolia Gagnep. is an original ornamental flower. To induce new phenotypic variations and breed new varieties with market value, ethyl methanesulfonate (EMS) was used to treat C. alismatifolia seedlings at various concentrations (0.0%, 0.1%, 0.5%, 1.0%, 1.5% and 2.0%) and for various durations (10, 15, 30, 60, 120 and 240 min). The treatment with 0.1% concentration for 10 min resulted in the highest survival rate, while treatments with 0.6% and 0.8% concentrations for 240 min could produce more diverse phenotypes. The treatment conditions of 2.0% EMS for 10 min and 0.5% EMS for 60 min are considered the semi-lethal doses for EMS mutagenesis of C. alismatifolia. This mutagenic process effectively induced diverse phenotypic changes, such as plant dwarfism, fewer bracts, leaf adhesion, chimera formation, altered coloration of the flower petal, and leaf fusion. We used a total of 16 simple sequence repeat (SSR) markers to analyze the genetic variation of the EMS-induced C. alismatifolia seedlings. The analysis revealed the following genetic parameters: the average polymorphism information content (PIC) was 0.6044, the number of alleles (Na) ranged from 2 to 3, the number of effective alleles (Ne) varied between 1.2195 and 2.9877, and the genetic diversity index (I) was 0.7532. This study promotes the mutation breeding program of C. alismatifolia. Significance of this study What is already known on this subject? Ethyl methanesulfonate (EMS) triggers mutations, while simple sequence repeat (SSR) markers are used to identify genetic variations. However, systematic research on the combined application of EMS mutagenesis and SSR analysis for breeding new varieties of Curcuma alismatifolia remains limited, particularly in optimizing treatment conditions and exploring genetic diversity in this species. What are the new findings? The study identifies the most effective EMS treatment durations for inducing diversity in C. alismatifolia: 2.0% for 10 min and 0.5% for 60 min. These treatments result in notable phenotypic changes such as dwarfism and altered leaf and flower traits. SSR analysis confirms significant genetic diversity, with an average polymorphism information content (PIC) of 0.6044 and a genetic diversity index of 0.7532 among mutants. What are the expected impacts on horticulture? The study aims to improve the breeding of C. alismatifolia by inducing genetic diversity and selecting traits such as dwarfism plants and unique leaf patterns, aiding in creating new cultivars for varied market needs. |
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| ISSN: | 1611-4426 1611-4434 |