Effect of Early and Late Interventions with Dietary Oils on Vascular and Neural Complications in a Type 2 Diabetic Rat Model
Aims. Determine the effect of dietary oils enriched in different mono- or polyunsaturated fatty acids, i.e., olive oil (18 : 1, oleic acid), safflower oil (18 : 2 n-6, linoleic acid), flaxseed oil (18 : 3 n-3, alpha linolenic acid), evening primrose oil (18 : 3 n-6, gamma linolenic acid), or menhade...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2019/5020465 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850160248677990400 |
|---|---|
| author | Lawrence Coppey Eric Davidson Hanna Shevalye Alexander Obrosov Mark Yorek |
| author_facet | Lawrence Coppey Eric Davidson Hanna Shevalye Alexander Obrosov Mark Yorek |
| author_sort | Lawrence Coppey |
| collection | DOAJ |
| description | Aims. Determine the effect of dietary oils enriched in different mono- or polyunsaturated fatty acids, i.e., olive oil (18 : 1, oleic acid), safflower oil (18 : 2 n-6, linoleic acid), flaxseed oil (18 : 3 n-3, alpha linolenic acid), evening primrose oil (18 : 3 n-6, gamma linolenic acid), or menhaden oil (20:5/22 : 6 n-3 eicosapentaenoic/docosahexaenoic acids), on vascular and neural complications in high-fat-fed low-dose streptozotocin-treated Sprague-Dawley rats, an animal model for late-stage type 2 diabetes. Materials and Methods. Rats were fed a high-fat diet (45% kcal as fat primarily derived from lard) for 8 weeks and then treated with a low dose of streptozotocin (30 mg/kg) in order to induce hyperglycemia. After an additional 8 (early intervention) or 20 (late intervention) weeks, the different groups of rats were fed diets with 1/2 of the kcal of fat derived from lard replaced by the different dietary oils. In addition, a control group fed a standard diet (4.25% kcal as fat) and a diabetic group maintained on the high-fat diet were maintained. The treatment period was approximately 16 weeks. The endpoints evaluated included vascular reactivity of epineurial arterioles, motor and sensory nerve conduction velocity, thermal and corneal sensitivity, and innervation of sensory nerves in the cornea and skin. Results. Our findings show that menhaden and flaxseed oil provided the greatest benefit for correcting peripheral nerve damage caused by diabetes, whereas enriching the high-fat diet with menhaden oil provided the most benefit to acetylcholine-mediated vascular relaxation of epineurial arterioles of the sciatic nerve. Enriching the diets with fatty acids derived from the other oils provided none to partial improvements. Conclusions. These studies imply that long-chain n-6 and n-3 polyunsaturated fatty acids could be an effective treatment for diabetic peripheral neuropathy with n-3 polyunsaturated fatty acids derived from fish oil being the most effective. |
| format | Article |
| id | doaj-art-b47fa2904ac342d19a226963e92d094c |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-b47fa2904ac342d19a226963e92d094c2025-08-20T02:23:12ZengWileyJournal of Diabetes Research2314-67452314-67532019-01-01201910.1155/2019/50204655020465Effect of Early and Late Interventions with Dietary Oils on Vascular and Neural Complications in a Type 2 Diabetic Rat ModelLawrence Coppey0Eric Davidson1Hanna Shevalye2Alexander Obrosov3Mark Yorek4Department of Internal Medicine, University of Iowa, Iowa City, IA, USADepartment of Internal Medicine, University of Iowa, Iowa City, IA, USADepartment of Internal Medicine, University of Iowa, Iowa City, IA, USADepartment of Internal Medicine, University of Iowa, Iowa City, IA, USADepartment of Internal Medicine, University of Iowa, Iowa City, IA, USAAims. Determine the effect of dietary oils enriched in different mono- or polyunsaturated fatty acids, i.e., olive oil (18 : 1, oleic acid), safflower oil (18 : 2 n-6, linoleic acid), flaxseed oil (18 : 3 n-3, alpha linolenic acid), evening primrose oil (18 : 3 n-6, gamma linolenic acid), or menhaden oil (20:5/22 : 6 n-3 eicosapentaenoic/docosahexaenoic acids), on vascular and neural complications in high-fat-fed low-dose streptozotocin-treated Sprague-Dawley rats, an animal model for late-stage type 2 diabetes. Materials and Methods. Rats were fed a high-fat diet (45% kcal as fat primarily derived from lard) for 8 weeks and then treated with a low dose of streptozotocin (30 mg/kg) in order to induce hyperglycemia. After an additional 8 (early intervention) or 20 (late intervention) weeks, the different groups of rats were fed diets with 1/2 of the kcal of fat derived from lard replaced by the different dietary oils. In addition, a control group fed a standard diet (4.25% kcal as fat) and a diabetic group maintained on the high-fat diet were maintained. The treatment period was approximately 16 weeks. The endpoints evaluated included vascular reactivity of epineurial arterioles, motor and sensory nerve conduction velocity, thermal and corneal sensitivity, and innervation of sensory nerves in the cornea and skin. Results. Our findings show that menhaden and flaxseed oil provided the greatest benefit for correcting peripheral nerve damage caused by diabetes, whereas enriching the high-fat diet with menhaden oil provided the most benefit to acetylcholine-mediated vascular relaxation of epineurial arterioles of the sciatic nerve. Enriching the diets with fatty acids derived from the other oils provided none to partial improvements. Conclusions. These studies imply that long-chain n-6 and n-3 polyunsaturated fatty acids could be an effective treatment for diabetic peripheral neuropathy with n-3 polyunsaturated fatty acids derived from fish oil being the most effective.http://dx.doi.org/10.1155/2019/5020465 |
| spellingShingle | Lawrence Coppey Eric Davidson Hanna Shevalye Alexander Obrosov Mark Yorek Effect of Early and Late Interventions with Dietary Oils on Vascular and Neural Complications in a Type 2 Diabetic Rat Model Journal of Diabetes Research |
| title | Effect of Early and Late Interventions with Dietary Oils on Vascular and Neural Complications in a Type 2 Diabetic Rat Model |
| title_full | Effect of Early and Late Interventions with Dietary Oils on Vascular and Neural Complications in a Type 2 Diabetic Rat Model |
| title_fullStr | Effect of Early and Late Interventions with Dietary Oils on Vascular and Neural Complications in a Type 2 Diabetic Rat Model |
| title_full_unstemmed | Effect of Early and Late Interventions with Dietary Oils on Vascular and Neural Complications in a Type 2 Diabetic Rat Model |
| title_short | Effect of Early and Late Interventions with Dietary Oils on Vascular and Neural Complications in a Type 2 Diabetic Rat Model |
| title_sort | effect of early and late interventions with dietary oils on vascular and neural complications in a type 2 diabetic rat model |
| url | http://dx.doi.org/10.1155/2019/5020465 |
| work_keys_str_mv | AT lawrencecoppey effectofearlyandlateinterventionswithdietaryoilsonvascularandneuralcomplicationsinatype2diabeticratmodel AT ericdavidson effectofearlyandlateinterventionswithdietaryoilsonvascularandneuralcomplicationsinatype2diabeticratmodel AT hannashevalye effectofearlyandlateinterventionswithdietaryoilsonvascularandneuralcomplicationsinatype2diabeticratmodel AT alexanderobrosov effectofearlyandlateinterventionswithdietaryoilsonvascularandneuralcomplicationsinatype2diabeticratmodel AT markyorek effectofearlyandlateinterventionswithdietaryoilsonvascularandneuralcomplicationsinatype2diabeticratmodel |