Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidy
It is believed that aneuploid embryos release cell-free DNA (cfDNA) into the blastocyst cavity during the self-correction process through the apoptotic mechanism. This study aimed to develop less invasive methods for predicting ploidy status by investigating how ploidy status affects blastocoel flui...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bioscientifica
2025-02-01
|
| Series: | Reproduction and Fertility |
| Subjects: | |
| Online Access: | https://raf.bioscientifica.com/view/journals/raf/6/1/RAF-24-0097.xml |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823858293447065600 |
|---|---|
| author | Fattaneh Khajehoseini Zahra Noormohammadi Poopak Eftekhari-Yazdi Hamid Gourabi Reza Pazhoomand Shirzad Hosseinishenatal Masood Bazrgar |
| author_facet | Fattaneh Khajehoseini Zahra Noormohammadi Poopak Eftekhari-Yazdi Hamid Gourabi Reza Pazhoomand Shirzad Hosseinishenatal Masood Bazrgar |
| author_sort | Fattaneh Khajehoseini |
| collection | DOAJ |
| description | It is believed that aneuploid embryos release cell-free DNA (cfDNA) into the blastocyst cavity during the self-correction process through the apoptotic mechanism. This study aimed to develop less invasive methods for predicting ploidy status by investigating how ploidy status affects blastocoel fluid DNA (BF-DNA) levels and apoptotic gene expression as indicators of embryo viability. Human blastocysts were classified into three groups; survivable embryo (SE), fatal single and double aneuploidy (FSDA) and multiple aneuploidy (MA) using array comparative genomic hybridization (array-CGH) by trophectoderm biopsy. Following BF aspiration and whole genome amplification, BF-DNA level was quantified. Apoptotic activity was assessed by measuring the genes TNFRSF10B, CASP2, BAX and CASP3 using real-time quantitative PCR. Day-5 intracytoplasmic sperm injection blastocysts were scored according to the Gardner and Schoolcraft system. BF-DNA levels were significantly higher in the MA vs SE group (P = 0.01), while these were not statistically significant differences between the MA and FSDA groups or between the FSDA and SE groups, P = 0.17 and P = 0.38, respectively. TNFRSF10B, CASP2 and CASP3 were overexpressed in the MA and FSDA groups compared to SE, while BAX was downregulated. We found a significant correlation between the amount of BF-DNA and apoptosis marker genes. No significant correlation was found between embryo morphology score and BF-DNA level, BF volume or apoptosis marker gene expression levels. We observed that the correlation between apoptotic activity and BF-DNA levels is influenced by the embryo’s ploidy status. These findings suggest that BF-DNA level evaluation can be applicable in selecting viable embryos for transfer. |
| format | Article |
| id | doaj-art-b47f2cc7752c428eb8e5090322432a69 |
| institution | Kabale University |
| issn | 2633-8386 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Reproduction and Fertility |
| spelling | doaj-art-b47f2cc7752c428eb8e5090322432a692025-02-11T12:33:24ZengBioscientificaReproduction and Fertility2633-83862025-02-016110.1530/RAF-24-00971Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidyFattaneh Khajehoseini0Zahra Noormohammadi1Poopak Eftekhari-Yazdi2Hamid Gourabi3Reza Pazhoomand4Shirzad Hosseinishenatal5Masood Bazrgar6Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranMedical Genetics Laboratory, Shiraz Fertility Center, Shiraz, IranDepartment of Embryology, Shiraz Fertility Center, Shiraz, IranDepartment of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranIt is believed that aneuploid embryos release cell-free DNA (cfDNA) into the blastocyst cavity during the self-correction process through the apoptotic mechanism. This study aimed to develop less invasive methods for predicting ploidy status by investigating how ploidy status affects blastocoel fluid DNA (BF-DNA) levels and apoptotic gene expression as indicators of embryo viability. Human blastocysts were classified into three groups; survivable embryo (SE), fatal single and double aneuploidy (FSDA) and multiple aneuploidy (MA) using array comparative genomic hybridization (array-CGH) by trophectoderm biopsy. Following BF aspiration and whole genome amplification, BF-DNA level was quantified. Apoptotic activity was assessed by measuring the genes TNFRSF10B, CASP2, BAX and CASP3 using real-time quantitative PCR. Day-5 intracytoplasmic sperm injection blastocysts were scored according to the Gardner and Schoolcraft system. BF-DNA levels were significantly higher in the MA vs SE group (P = 0.01), while these were not statistically significant differences between the MA and FSDA groups or between the FSDA and SE groups, P = 0.17 and P = 0.38, respectively. TNFRSF10B, CASP2 and CASP3 were overexpressed in the MA and FSDA groups compared to SE, while BAX was downregulated. We found a significant correlation between the amount of BF-DNA and apoptosis marker genes. No significant correlation was found between embryo morphology score and BF-DNA level, BF volume or apoptosis marker gene expression levels. We observed that the correlation between apoptotic activity and BF-DNA levels is influenced by the embryo’s ploidy status. These findings suggest that BF-DNA level evaluation can be applicable in selecting viable embryos for transfer.https://raf.bioscientifica.com/view/journals/raf/6/1/RAF-24-0097.xmlblastocoel fluidaneuploidyapoptosiscell-free dnagene expression |
| spellingShingle | Fattaneh Khajehoseini Zahra Noormohammadi Poopak Eftekhari-Yazdi Hamid Gourabi Reza Pazhoomand Shirzad Hosseinishenatal Masood Bazrgar Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidy Reproduction and Fertility blastocoel fluid aneuploidy apoptosis cell-free dna gene expression |
| title | Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidy |
| title_full | Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidy |
| title_fullStr | Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidy |
| title_full_unstemmed | Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidy |
| title_short | Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidy |
| title_sort | correlation among blastocoel fluid dna level apoptotic genes expression and preimplantation aneuploidy |
| topic | blastocoel fluid aneuploidy apoptosis cell-free dna gene expression |
| url | https://raf.bioscientifica.com/view/journals/raf/6/1/RAF-24-0097.xml |
| work_keys_str_mv | AT fattanehkhajehoseini correlationamongblastocoelfluiddnalevelapoptoticgenesexpressionandpreimplantationaneuploidy AT zahranoormohammadi correlationamongblastocoelfluiddnalevelapoptoticgenesexpressionandpreimplantationaneuploidy AT poopakeftekhariyazdi correlationamongblastocoelfluiddnalevelapoptoticgenesexpressionandpreimplantationaneuploidy AT hamidgourabi correlationamongblastocoelfluiddnalevelapoptoticgenesexpressionandpreimplantationaneuploidy AT rezapazhoomand correlationamongblastocoelfluiddnalevelapoptoticgenesexpressionandpreimplantationaneuploidy AT shirzadhosseinishenatal correlationamongblastocoelfluiddnalevelapoptoticgenesexpressionandpreimplantationaneuploidy AT masoodbazrgar correlationamongblastocoelfluiddnalevelapoptoticgenesexpressionandpreimplantationaneuploidy |