Study on β-momorcharin molecular modeling
The amino acid composition and hydrophilism and hydrophobicity of β-momorcharin were analyzed by DNAMAN software, and its basic features, secondary and tertiary structures, a coiled coil area and a PEST sequence were predicted by bioinformatics online websites, and the surface potential and accessib...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2011-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.04.007 |
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| Summary: | The amino acid composition and hydrophilism and hydrophobicity of β-momorcharin were analyzed by DNAMAN software, and its basic features, secondary and tertiary structures, a coiled coil area and a PEST sequence were predicted by bioinformatics online websites, and the surface potential and accessibility were analysed by bioinformatics software, then the structural quality analysis and detection evaluation on the modeling result were carried out by establishing a Ramachandran graph. The result showed that the β-momorcharin comprised 249 amino acids and had isoelectric points of 9.19, and the hydrophilic area and the hydrophobic area were distributed in a staggered manner; the β-momorcharin belonged to stable protein as the extinction coefficient thereof was 280 nm, and the average hydrophilism was -0.138; the secondary structure comprised 42.57% of α-helix, 20.48% of extended strand and 36.95% of irregular coil; there were three sections of coiled coil areas, five sections of non-PEST sequences, and no stable dipolymer or tripolymer coiled coil; the space distribution of tertiary structure, surface potential and accessibility of β-momorcharin were modeled, and the Ramachandran graph detection showed that the structure of the model met the rules of stereochemistry. |
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| ISSN: | 1008-9209 2097-5155 |