Comparative effect of dose escalation of nanocapsulated ivermectin against mange in rabbits

The present study aimed to investigate the effects of nano-capsulated ivermectin on the liver and kidney function and oxidative status in mite infested-rabbits, compared to ivermectin. Additionally, the ivermectin residue profile in adipose tissue, liver, muscle, and kidney was evaluated. For this p...

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Main Authors: Azza Abdelmoteleb, Dalia Elmasry, Fatma Amro, Reham Mahmoud
Format: Article
Language:English
Published: German Multidisciplinary Publishing Center (GMPC) 2022-01-01
Series:German Journal of Veterinary Research
Online Access:https://doi.org/10.51585/gjvr.2022.4.0043
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author Azza Abdelmoteleb
Dalia Elmasry
Fatma Amro
Reham Mahmoud
author_facet Azza Abdelmoteleb
Dalia Elmasry
Fatma Amro
Reham Mahmoud
author_sort Azza Abdelmoteleb
collection DOAJ
description The present study aimed to investigate the effects of nano-capsulated ivermectin on the liver and kidney function and oxidative status in mite infested-rabbits, compared to ivermectin. Additionally, the ivermectin residue profile in adipose tissue, liver, muscle, and kidney was evaluated. For this purpose, nano-capsulated ivermectin was prepared and characterized using high-resolution transmission electron microscopy (HRTEM) and cytotoxicity assay on Vero cells. To assess the effect of dose escalation of nano-capsulated ivermectin, one-hundred naturally mite-infested male rabbits were divided into four groups (G1-G4; n=25). Rabbits kept in G1 were left untreated (positive control), while rabbits kept in G2 and G3 received subcutaneously 200 and 400 μg/kg body weight ivermectin, respectively, at zero-day and repeated after two weeks of the first injection. Rabbits in G4 were treated with 200 μg/kg nano-capsulated ivermectin at zero day as a single dose. Additionally, twenty-five healthy male rabbits (G0) were used as a negative control. The efficacy was assessed based on clinical manifestations, liver and kidney function, and oxidative stress parameters. Ivermectin residues were measured in fat, liver, muscle, and kidney using high-performance liquid chromatography (HPLC). Results showed that the size of the nano-capsulated ivermectin was 35.4 nm with a narrow size distribution of 0.578 polydispersity indexes. A significant improvement in liver and kidney functions (P
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spelling doaj-art-b4772e9675ea401daaa731e68c73ffd32025-08-20T02:02:40ZengGerman Multidisciplinary Publishing Center (GMPC)German Journal of Veterinary Research2703-13222022-01-0181510.51585/gjvr.2022.4.0043Comparative effect of dose escalation of nanocapsulated ivermectin against mange in rabbitsAzza AbdelmotelebDalia ElmasryFatma AmroReham MahmoudThe present study aimed to investigate the effects of nano-capsulated ivermectin on the liver and kidney function and oxidative status in mite infested-rabbits, compared to ivermectin. Additionally, the ivermectin residue profile in adipose tissue, liver, muscle, and kidney was evaluated. For this purpose, nano-capsulated ivermectin was prepared and characterized using high-resolution transmission electron microscopy (HRTEM) and cytotoxicity assay on Vero cells. To assess the effect of dose escalation of nano-capsulated ivermectin, one-hundred naturally mite-infested male rabbits were divided into four groups (G1-G4; n=25). Rabbits kept in G1 were left untreated (positive control), while rabbits kept in G2 and G3 received subcutaneously 200 and 400 μg/kg body weight ivermectin, respectively, at zero-day and repeated after two weeks of the first injection. Rabbits in G4 were treated with 200 μg/kg nano-capsulated ivermectin at zero day as a single dose. Additionally, twenty-five healthy male rabbits (G0) were used as a negative control. The efficacy was assessed based on clinical manifestations, liver and kidney function, and oxidative stress parameters. Ivermectin residues were measured in fat, liver, muscle, and kidney using high-performance liquid chromatography (HPLC). Results showed that the size of the nano-capsulated ivermectin was 35.4 nm with a narrow size distribution of 0.578 polydispersity indexes. A significant improvement in liver and kidney functions (Phttps://doi.org/10.51585/gjvr.2022.4.0043
spellingShingle Azza Abdelmoteleb
Dalia Elmasry
Fatma Amro
Reham Mahmoud
Comparative effect of dose escalation of nanocapsulated ivermectin against mange in rabbits
German Journal of Veterinary Research
title Comparative effect of dose escalation of nanocapsulated ivermectin against mange in rabbits
title_full Comparative effect of dose escalation of nanocapsulated ivermectin against mange in rabbits
title_fullStr Comparative effect of dose escalation of nanocapsulated ivermectin against mange in rabbits
title_full_unstemmed Comparative effect of dose escalation of nanocapsulated ivermectin against mange in rabbits
title_short Comparative effect of dose escalation of nanocapsulated ivermectin against mange in rabbits
title_sort comparative effect of dose escalation of nanocapsulated ivermectin against mange in rabbits
url https://doi.org/10.51585/gjvr.2022.4.0043
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AT fatmaamro comparativeeffectofdoseescalationofnanocapsulatedivermectinagainstmangeinrabbits
AT rehammahmoud comparativeeffectofdoseescalationofnanocapsulatedivermectinagainstmangeinrabbits