Regulation of Med1 protein by overexpression of BAP1 in breast cancer cells
Med1 binds to a nuclear receptor and regulates transcription. Elevated Med1 protein expression promotes cancer growth in hormone-dependent breast and prostate cancers. Med1 protein expression was investigated by deubiquitinating enzymes (DUBs) overexpression in breast cancer cell lines. Various DNA...
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Taylor & Francis Group
2024-12-01
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| Series: | Molecular & Cellular Oncology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23723556.2024.2347827 |
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| author | Hyunju Kim |
| author_facet | Hyunju Kim |
| author_sort | Hyunju Kim |
| collection | DOAJ |
| description | Med1 binds to a nuclear receptor and regulates transcription. Elevated Med1 protein expression promotes cancer growth in hormone-dependent breast and prostate cancers. Med1 protein expression was investigated by deubiquitinating enzymes (DUBs) overexpression in breast cancer cell lines. Various DNA constructs of SRT-DUBs were overexpressed in the MCF7 cell line, and Med1 protein expression was investigated by western blotting. The cell growth and in vitro invasion assay were performed in BRCA1-associated protein 1 (BAP1) wild type and mutant (C91A) overexpressed cells. Ubiquitination of the Med1 protein was observed, and Med1 protein expression and transcriptional activity were verified by various DUBs overexpressed. Although Med1 protein expression increased upon the overexpression of BAP1, it was not affected by the overexpression of BAP1 mutant (C91A). BAP1 was increased by the E2 treatment, which has an important effect on the breast cancer growth, and cell growth was decreased by BAP1 C91A overexpression. However, metastatic capacities were decreased by BAP1. In addition, the binding between the Med1 and the BAP1 protein was observed. These data suggested that BAP1 regulated Med1 protein expression in breast cancer cells and involved in cancer cell growth and metastasis by binding to Med1 protein. |
| format | Article |
| id | doaj-art-b46dce4ccebe47a7917818b4acc134c0 |
| institution | OA Journals |
| issn | 2372-3556 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Molecular & Cellular Oncology |
| spelling | doaj-art-b46dce4ccebe47a7917818b4acc134c02025-08-20T01:58:48ZengTaylor & Francis GroupMolecular & Cellular Oncology2372-35562024-12-0111110.1080/23723556.2024.2347827Regulation of Med1 protein by overexpression of BAP1 in breast cancer cellsHyunju Kim0Department of Health Sciences, The Graduate School of Dong-A University, Busan, South KoreaMed1 binds to a nuclear receptor and regulates transcription. Elevated Med1 protein expression promotes cancer growth in hormone-dependent breast and prostate cancers. Med1 protein expression was investigated by deubiquitinating enzymes (DUBs) overexpression in breast cancer cell lines. Various DNA constructs of SRT-DUBs were overexpressed in the MCF7 cell line, and Med1 protein expression was investigated by western blotting. The cell growth and in vitro invasion assay were performed in BRCA1-associated protein 1 (BAP1) wild type and mutant (C91A) overexpressed cells. Ubiquitination of the Med1 protein was observed, and Med1 protein expression and transcriptional activity were verified by various DUBs overexpressed. Although Med1 protein expression increased upon the overexpression of BAP1, it was not affected by the overexpression of BAP1 mutant (C91A). BAP1 was increased by the E2 treatment, which has an important effect on the breast cancer growth, and cell growth was decreased by BAP1 C91A overexpression. However, metastatic capacities were decreased by BAP1. In addition, the binding between the Med1 and the BAP1 protein was observed. These data suggested that BAP1 regulated Med1 protein expression in breast cancer cells and involved in cancer cell growth and metastasis by binding to Med1 protein.https://www.tandfonline.com/doi/10.1080/23723556.2024.2347827Med1BAP1breast cancer cellsprotein expression regulationdeubiquitinating enzyme |
| spellingShingle | Hyunju Kim Regulation of Med1 protein by overexpression of BAP1 in breast cancer cells Molecular & Cellular Oncology Med1 BAP1 breast cancer cells protein expression regulation deubiquitinating enzyme |
| title | Regulation of Med1 protein by overexpression of BAP1 in breast cancer cells |
| title_full | Regulation of Med1 protein by overexpression of BAP1 in breast cancer cells |
| title_fullStr | Regulation of Med1 protein by overexpression of BAP1 in breast cancer cells |
| title_full_unstemmed | Regulation of Med1 protein by overexpression of BAP1 in breast cancer cells |
| title_short | Regulation of Med1 protein by overexpression of BAP1 in breast cancer cells |
| title_sort | regulation of med1 protein by overexpression of bap1 in breast cancer cells |
| topic | Med1 BAP1 breast cancer cells protein expression regulation deubiquitinating enzyme |
| url | https://www.tandfonline.com/doi/10.1080/23723556.2024.2347827 |
| work_keys_str_mv | AT hyunjukim regulationofmed1proteinbyoverexpressionofbap1inbreastcancercells |