Source of Explant and Light Spectrum Influence in Adventitious Shoot Regeneration of <i>Prunus salicina</i> Lindl. (Japanese plum)
Light influence on shoot regeneration in <i>Prunus salicina</i> is a complex interaction that has been studied for the first time. Japanese plum plants were regenerated from calli and seeds of the scion cultivar ‘Victoria’. The effect of four different light spectra (white, blue, red, an...
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2025-07-01
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| author | Carmen López-Sierra José E. Cos-Terrer Miriam Romero-Muñoz Margarita Pérez-Jiménez |
| author_facet | Carmen López-Sierra José E. Cos-Terrer Miriam Romero-Muñoz Margarita Pérez-Jiménez |
| author_sort | Carmen López-Sierra |
| collection | DOAJ |
| description | Light influence on shoot regeneration in <i>Prunus salicina</i> is a complex interaction that has been studied for the first time. Japanese plum plants were regenerated from calli and seeds of the scion cultivar ‘Victoria’. The effect of four different light spectra (white, blue, red, and mixed), along with three 6-benzyladenine (BA) concentrations (1, 1.5, and 2 mg L<sup>−1</sup>), was studied in these two sources of explants. Organogenic calli were derived from the base of stem explants of the scion cultivar ‘Victoria’, whereas cotyledons and embryogenic axis slices were used as seed explants. Calli cultured with 2 mg L<sup>−1</sup> of BA and mixed light or 2.5 mg L<sup>−1</sup> of BA and control light showed the highest regeneration rates, with no significant differences compared to other treatments. Seed explants exposed to 2.5 mg L<sup>−1</sup> of BA and red light exhibited significantly higher organogenesis. In comparison, those in 1.5 mg L<sup>−1</sup> of BA with blue light or 2.5 mg L<sup>−1</sup> of BA with mixed/control light showed no regeneration. BA concentration did not have a significant effect in the induction of somatic shoots from any explant source. In contrast, a strong interaction between light and BA was noticed. This work presents a protocol that can be applied in transformation and editing research as light spectrum studies continue to advance. |
| format | Article |
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| publishDate | 2025-07-01 |
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| spelling | doaj-art-b4585ae33f0a49d4a6cad0aa482e4b882025-08-20T02:47:22ZengMDPI AGPlants2223-77472025-07-011414223010.3390/plants14142230Source of Explant and Light Spectrum Influence in Adventitious Shoot Regeneration of <i>Prunus salicina</i> Lindl. (Japanese plum)Carmen López-Sierra0José E. Cos-Terrer1Miriam Romero-Muñoz2Margarita Pérez-Jiménez3Department of Plant Biotechnology, Genomics and Breeding, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), 30150 Murcia, SpainDepartment of Plant Biotechnology, Genomics and Breeding, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), 30150 Murcia, SpainDepartment of Plant Biotechnology, Genomics and Breeding, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), 30150 Murcia, SpainDepartment of Plant Biotechnology, Genomics and Breeding, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), 30150 Murcia, SpainLight influence on shoot regeneration in <i>Prunus salicina</i> is a complex interaction that has been studied for the first time. Japanese plum plants were regenerated from calli and seeds of the scion cultivar ‘Victoria’. The effect of four different light spectra (white, blue, red, and mixed), along with three 6-benzyladenine (BA) concentrations (1, 1.5, and 2 mg L<sup>−1</sup>), was studied in these two sources of explants. Organogenic calli were derived from the base of stem explants of the scion cultivar ‘Victoria’, whereas cotyledons and embryogenic axis slices were used as seed explants. Calli cultured with 2 mg L<sup>−1</sup> of BA and mixed light or 2.5 mg L<sup>−1</sup> of BA and control light showed the highest regeneration rates, with no significant differences compared to other treatments. Seed explants exposed to 2.5 mg L<sup>−1</sup> of BA and red light exhibited significantly higher organogenesis. In comparison, those in 1.5 mg L<sup>−1</sup> of BA with blue light or 2.5 mg L<sup>−1</sup> of BA with mixed/control light showed no regeneration. BA concentration did not have a significant effect in the induction of somatic shoots from any explant source. In contrast, a strong interaction between light and BA was noticed. This work presents a protocol that can be applied in transformation and editing research as light spectrum studies continue to advance.https://www.mdpi.com/2223-7747/14/14/2230morphogenesisde novo regenerationLEDs6-benzyladeninesomatic shoots |
| spellingShingle | Carmen López-Sierra José E. Cos-Terrer Miriam Romero-Muñoz Margarita Pérez-Jiménez Source of Explant and Light Spectrum Influence in Adventitious Shoot Regeneration of <i>Prunus salicina</i> Lindl. (Japanese plum) Plants morphogenesis de novo regeneration LEDs 6-benzyladenine somatic shoots |
| title | Source of Explant and Light Spectrum Influence in Adventitious Shoot Regeneration of <i>Prunus salicina</i> Lindl. (Japanese plum) |
| title_full | Source of Explant and Light Spectrum Influence in Adventitious Shoot Regeneration of <i>Prunus salicina</i> Lindl. (Japanese plum) |
| title_fullStr | Source of Explant and Light Spectrum Influence in Adventitious Shoot Regeneration of <i>Prunus salicina</i> Lindl. (Japanese plum) |
| title_full_unstemmed | Source of Explant and Light Spectrum Influence in Adventitious Shoot Regeneration of <i>Prunus salicina</i> Lindl. (Japanese plum) |
| title_short | Source of Explant and Light Spectrum Influence in Adventitious Shoot Regeneration of <i>Prunus salicina</i> Lindl. (Japanese plum) |
| title_sort | source of explant and light spectrum influence in adventitious shoot regeneration of i prunus salicina i lindl japanese plum |
| topic | morphogenesis de novo regeneration LEDs 6-benzyladenine somatic shoots |
| url | https://www.mdpi.com/2223-7747/14/14/2230 |
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