Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish
Obesity is a highly prevalent disorder with complex aetiology. Therefore, studying its associated cellular and molecular pathways may be aided by analysing genetic tractable diseases. In this context, the study of ciliopathies such as Bardet–Biedl syndrome has highlighted the relevance of primary ci...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Adipocyte |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21623945.2024.2435862 |
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| author | Lucía Guggeri Ileana Sosa-Redaelli Magdalena Cárdenas-Rodríguez Martina Alonso Gisell González Hugo Naya Victoria Prieto-Echagüe Paola Lepanto Jose L. Badano |
| author_facet | Lucía Guggeri Ileana Sosa-Redaelli Magdalena Cárdenas-Rodríguez Martina Alonso Gisell González Hugo Naya Victoria Prieto-Echagüe Paola Lepanto Jose L. Badano |
| author_sort | Lucía Guggeri |
| collection | DOAJ |
| description | Obesity is a highly prevalent disorder with complex aetiology. Therefore, studying its associated cellular and molecular pathways may be aided by analysing genetic tractable diseases. In this context, the study of ciliopathies such as Bardet–Biedl syndrome has highlighted the relevance of primary cilia in obesity, both in the central nervous system and peripheral tissues. Based on our previous in vitro results supporting the role of a novel Bbs4-cilia-Fstl1 axis in adipocyte differentiation, we evaluated the in vivo relevance of the zebrafish orthologous genes fstl1a and fstl1b in primary cilia and adipose tissue development. Using a combination of knockdowns and a new fstl1a mutant line, we show that fstl1a promotes primary cilia formation in early embryos and participates in adipose tissue formation in larvae. We also show that fstl1b partially compensates for the loss of fstl1a. Moreover, in high fat diet, fstl1a depletion affects the expression of differentiation and mature adipocyte markers. These results agree with our previous in vitro data and provide further support for the role of FSTL1 as a regulator of adipose tissue formation. Dissecting the exact biological role of proteins such as FSTL1 will likely contribute to understand obesity onset and presentation. |
| format | Article |
| id | doaj-art-b44af926083b4f2f94ebc55100f5afcf |
| institution | OA Journals |
| issn | 2162-3945 2162-397X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj-art-b44af926083b4f2f94ebc55100f5afcf2025-08-20T02:30:41ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2024-12-0113110.1080/21623945.2024.2435862Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafishLucía Guggeri0Ileana Sosa-Redaelli1Magdalena Cárdenas-Rodríguez2Martina Alonso3Gisell González4Hugo Naya5Victoria Prieto-Echagüe6Paola Lepanto7Jose L. Badano8Human Molecular Genetics Laboratory, Institut Pasteur de Montevideo, Montevideo, UruguayHuman Molecular Genetics Laboratory, Institut Pasteur de Montevideo, Montevideo, UruguayHuman Molecular Genetics Laboratory, Institut Pasteur de Montevideo, Montevideo, UruguayHuman Molecular Genetics Laboratory, Institut Pasteur de Montevideo, Montevideo, UruguayZebrafish Laboratory, Institut Pasteur de Montevideo, Montevideo, UruguayBioinformatics Unit, Institut Pasteur de Montevideo, Montevideo, UruguayHuman Molecular Genetics Laboratory, Institut Pasteur de Montevideo, Montevideo, UruguayHuman Molecular Genetics Laboratory, Institut Pasteur de Montevideo, Montevideo, UruguayHuman Molecular Genetics Laboratory, Institut Pasteur de Montevideo, Montevideo, UruguayObesity is a highly prevalent disorder with complex aetiology. Therefore, studying its associated cellular and molecular pathways may be aided by analysing genetic tractable diseases. In this context, the study of ciliopathies such as Bardet–Biedl syndrome has highlighted the relevance of primary cilia in obesity, both in the central nervous system and peripheral tissues. Based on our previous in vitro results supporting the role of a novel Bbs4-cilia-Fstl1 axis in adipocyte differentiation, we evaluated the in vivo relevance of the zebrafish orthologous genes fstl1a and fstl1b in primary cilia and adipose tissue development. Using a combination of knockdowns and a new fstl1a mutant line, we show that fstl1a promotes primary cilia formation in early embryos and participates in adipose tissue formation in larvae. We also show that fstl1b partially compensates for the loss of fstl1a. Moreover, in high fat diet, fstl1a depletion affects the expression of differentiation and mature adipocyte markers. These results agree with our previous in vitro data and provide further support for the role of FSTL1 as a regulator of adipose tissue formation. Dissecting the exact biological role of proteins such as FSTL1 will likely contribute to understand obesity onset and presentation.https://www.tandfonline.com/doi/10.1080/21623945.2024.2435862Adipose tissueciliazebrafishBardet–Biedl syndromefollistatin-like 1 |
| spellingShingle | Lucía Guggeri Ileana Sosa-Redaelli Magdalena Cárdenas-Rodríguez Martina Alonso Gisell González Hugo Naya Victoria Prieto-Echagüe Paola Lepanto Jose L. Badano Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish Adipocyte Adipose tissue cilia zebrafish Bardet–Biedl syndrome follistatin-like 1 |
| title | Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish |
| title_full | Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish |
| title_fullStr | Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish |
| title_full_unstemmed | Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish |
| title_short | Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish |
| title_sort | follistatin like 1 fstl1 regulates adipose tissue development in zebrafish |
| topic | Adipose tissue cilia zebrafish Bardet–Biedl syndrome follistatin-like 1 |
| url | https://www.tandfonline.com/doi/10.1080/21623945.2024.2435862 |
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