Seroprevalence and molecular diagnosis of Toxoplasma gondii infection among blood donors in southern Iran
Introduction: Toxoplasma gondii is a protozoan parasite which can be transmitted to human through a variety of routes including blood transfusion. This cross sectional study aimed to evaluate the seroprevalence of Toxoplasma infection and related epidemiological features among healthy blood donors....
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The Journal of Infection in Developing Countries
2014-04-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/3831 |
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| author | Bahador Sarkari Reza Shafiei Mani Zare Sattar Sohrabpour Leila Kasraian |
| author_facet | Bahador Sarkari Reza Shafiei Mani Zare Sattar Sohrabpour Leila Kasraian |
| author_sort | Bahador Sarkari |
| collection | DOAJ |
| description | Introduction: Toxoplasma gondii is a protozoan parasite which can be transmitted to human through a variety of routes including blood transfusion. This cross sectional study aimed to evaluate the seroprevalence of Toxoplasma infection and related epidemiological features among healthy blood donors.
Methodology: A total of 1,480 healthy blood donors from five blood service centers in Fars province were analyzed for anti-Toxoplasma antibodies. Blood samples were tested for anti-T. gondii IgG and IgM antibodies by enzyme immunoassay. IgM-positive samples were also tested for the presence of Toxoplasma DNA by Polymerase Chain Reaction (PCR). Demographic characteristics of participants were also recorded during samples collection.
Results: Anti T. gondii antibodies were detected in sera of 286 out of 1,480 blood donors corresponding to an overall seroprevalence of 19.3% in this population. From these, 182 (12.3%) were seropositive only for IgG, 81 (5.47%) were seropositive only for IgM and 23 (1.6%) were positive for both IgG and IgM. PCR detected active parasitemia in two (1.9%) of the IgM-positive subjects. Age, place of residence and level of education were statistically significant (p < 0.05) with seropositivity to Toxoplasma.
Conclusions: Our results highlighted that asymptomatic blood donors, especially those with active parasitemia, may constitute a significant risk of transmitting toxoplasmosis to susceptible recipients.
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| format | Article |
| id | doaj-art-b441e09d232d4e5da673118f5ad1b951 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-04-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-b441e09d232d4e5da673118f5ad1b9512025-08-20T02:27:20ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-04-0180410.3855/jidc.3831Seroprevalence and molecular diagnosis of Toxoplasma gondii infection among blood donors in southern IranBahador Sarkari0Reza Shafiei1Mani Zare2Sattar Sohrabpour3Leila Kasraian4Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, IranSchool of Medicine, Shiraz University of Medical Sciences, Shiraz, IranSchool of Medicine, Shiraz University of Medical Sciences, Shiraz, IranSchool of Medicine, Shiraz University of Medical Sciences, Shiraz, IranShiraz Blood Transfusion Organization, Shiraz, IranIntroduction: Toxoplasma gondii is a protozoan parasite which can be transmitted to human through a variety of routes including blood transfusion. This cross sectional study aimed to evaluate the seroprevalence of Toxoplasma infection and related epidemiological features among healthy blood donors. Methodology: A total of 1,480 healthy blood donors from five blood service centers in Fars province were analyzed for anti-Toxoplasma antibodies. Blood samples were tested for anti-T. gondii IgG and IgM antibodies by enzyme immunoassay. IgM-positive samples were also tested for the presence of Toxoplasma DNA by Polymerase Chain Reaction (PCR). Demographic characteristics of participants were also recorded during samples collection. Results: Anti T. gondii antibodies were detected in sera of 286 out of 1,480 blood donors corresponding to an overall seroprevalence of 19.3% in this population. From these, 182 (12.3%) were seropositive only for IgG, 81 (5.47%) were seropositive only for IgM and 23 (1.6%) were positive for both IgG and IgM. PCR detected active parasitemia in two (1.9%) of the IgM-positive subjects. Age, place of residence and level of education were statistically significant (p < 0.05) with seropositivity to Toxoplasma. Conclusions: Our results highlighted that asymptomatic blood donors, especially those with active parasitemia, may constitute a significant risk of transmitting toxoplasmosis to susceptible recipients. https://jidc.org/index.php/journal/article/view/3831Toxoplasma gondiiblood donorsseroprevalence |
| spellingShingle | Bahador Sarkari Reza Shafiei Mani Zare Sattar Sohrabpour Leila Kasraian Seroprevalence and molecular diagnosis of Toxoplasma gondii infection among blood donors in southern Iran Journal of Infection in Developing Countries Toxoplasma gondii blood donors seroprevalence |
| title | Seroprevalence and molecular diagnosis of Toxoplasma gondii infection among blood donors in southern Iran |
| title_full | Seroprevalence and molecular diagnosis of Toxoplasma gondii infection among blood donors in southern Iran |
| title_fullStr | Seroprevalence and molecular diagnosis of Toxoplasma gondii infection among blood donors in southern Iran |
| title_full_unstemmed | Seroprevalence and molecular diagnosis of Toxoplasma gondii infection among blood donors in southern Iran |
| title_short | Seroprevalence and molecular diagnosis of Toxoplasma gondii infection among blood donors in southern Iran |
| title_sort | seroprevalence and molecular diagnosis of toxoplasma gondii infection among blood donors in southern iran |
| topic | Toxoplasma gondii blood donors seroprevalence |
| url | https://jidc.org/index.php/journal/article/view/3831 |
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