The serological survey on bluetongue virus infection in sheep
A swn of 86 blood sera of sheep were collected from Konya Livestock Research Centre. The blood sera were examined to detennine the presence of neutralizing antibodies to BTV (Bluetongue Virus), type SA<sub>4</sub> by microneutralization test. BHK (Baby Hamster Kidney) permanent eeliline...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Selcuk University Press
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Series: | Eurasian Journal of Veterinary Sciences |
Online Access: | http://eurasianjvetsci.org/pdf.php3?id=725 |
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Summary: | A swn of 86 blood sera of sheep were collected
from Konya Livestock Research Centre. The blood sera
were examined to detennine the presence of neutralizing antibodies
to BTV (Bluetongue Virus), type SA<sub>4</sub> by microneutralization
test. BHK (Baby Hamster Kidney) permanent eeliline
was used for production and titration of BTV and neutralisation
test. Glascow Eagle's MEM (Minimum Essential Medium)
with 10 % calf sera for cell medium and Glascow Eagle's
MEM without sera for virus medium were used in this research.
The blood sera were tested at the rate of 1/10 dilution.
At the end of the microneutralization test, the 31 (36.04 %)
sheep blood were found seropozitive to BTV, type SA<sub>4</sub>. |
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ISSN: | 1309-6958 2146-1953 |