Estimation of miRNA-21, miRNA-422, miRNA‐142-3p, and miR-181c Gene Expression as Potential Biomarkers of Osteoporosis in Premenopausal Women
Background: MicroRNAs (miRNAs) regulate several biological pathways in osteoporosis patients. The study aimed to estimate the correlation of miRNA-21, miRNA-422, miRNA‐142-3p, and miR-181c with osteoporosis in premenopausal women. Patients and Methods: This study was performed on 60 osteoporosis...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
College of medicine/ University of Diyala
2025-06-01
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| Series: | Diyala Journal of Medicine |
| Subjects: | |
| Online Access: | http://djm.uodiyala.edu.iq/index.php/djm/article/view/1295 |
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| Summary: | Background: MicroRNAs (miRNAs) regulate several biological pathways in osteoporosis patients. The study aimed to estimate the correlation of miRNA-21, miRNA-422, miRNA‐142-3p, and miR-181c with osteoporosis in premenopausal women.
Patients and Methods: This study was performed on 60 osteoporosis premenopausal and 30 healthy women. Determination of osteocalcin (OC), deoxypyridinoline (DPD), and bone-specific alkaline phosphatase (BAP) were done by ELISA, and estimation of miRNA gene expression was done by using qRT-PCR.
Results: OC, DPD, and BAP levels indicated a significant decrease in osteoporosis women. In addition, our data showed that miRNA-21 (p=0.0001, r=-0.5585) and miRNA-422 (p=0.0035, r=-0.3715) have a high expression and negative correlation with BMD. Meanwhile, the miRNA-142-3p (p=0.0136, r=0.3089) and miRNA-181c (p=0.0401, r=-0.2685) have decreased expression and positive correlation with BMD value.
Conclusion: The results of this study indicated a clear association of miRNA-21 and miRNA-422 in osteoporosis thus they may be useful as biomarkers for osteoporosis.
Keywords: Osteoporosis, miRNA-21, miRNA-422, miRNA‐142-3p, and miR-181c.
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| ISSN: | 2219-9764 2617-8982 |