Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos
Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2014/409021 |
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| author | Jitong Guo Baojiang Wu Shuyu Li Siqin Bao Lixia Zhao Shuxiang Hu Wei Sun Jie Su Yanfeng Dai Xihe Li |
| author_facet | Jitong Guo Baojiang Wu Shuyu Li Siqin Bao Lixia Zhao Shuxiang Hu Wei Sun Jie Su Yanfeng Dai Xihe Li |
| author_sort | Jitong Guo |
| collection | DOAJ |
| description | Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells and induced pluripotent stem cells, respectively. Although a significantly higher chimera rate resulted from blastocyst injection, the highest germline contribution resulted from injection of 8-cell embryos with embryonic stem cells. The fully agouti colored chimeras were generated from both injection and coculture of 8-cell embryos with embryonic stem cells. Additionally, microsatellite DNA screening showed that the fully agouti colored chimeras were fully embryonic stem cell derived mice. Unlike embryonic stem cells, the mouse chimeras were only generated from injection of 8-cell embryos with induced pluripotent stem cells and none of these showed germline transmission. The results indicated that injection of 8-cell embryos is the most efficient method for assessing stem cell pluripotency and generating induced pluripotent stem cell chimeras, embryonic stem cell chimeras with germline transmission, and fully mouse embryonic stem cell derived mice. |
| format | Article |
| id | doaj-art-b41f9d890adf49ad86375eb4aae97537 |
| institution | DOAJ |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b41f9d890adf49ad86375eb4aae975372025-08-20T03:20:51ZengWileyStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/409021409021Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of EmbryosJitong Guo0Baojiang Wu1Shuyu Li2Siqin Bao3Lixia Zhao4Shuxiang Hu5Wei Sun6Jie Su7Yanfeng Dai8Xihe Li9Research Center for Animal Genetic Resources of Mongolia Plateau, Inner Mongolia University, Hohhot 010021, ChinaInner Mongolia Saikexing Reproductive Biotechnology Co., Ltd., Helingeer 011517, ChinaInner Mongolia Saikexing Reproductive Biotechnology Co., Ltd., Helingeer 011517, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, Inner Mongolia University, Hohhot 010021, ChinaInner Mongolia Saikexing Reproductive Biotechnology Co., Ltd., Helingeer 011517, ChinaInner Mongolia Saikexing Reproductive Biotechnology Co., Ltd., Helingeer 011517, ChinaInner Mongolia Saikexing Reproductive Biotechnology Co., Ltd., Helingeer 011517, ChinaInner Mongolia Saikexing Reproductive Biotechnology Co., Ltd., Helingeer 011517, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, Inner Mongolia University, Hohhot 010021, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, Inner Mongolia University, Hohhot 010021, ChinaBlastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells and induced pluripotent stem cells, respectively. Although a significantly higher chimera rate resulted from blastocyst injection, the highest germline contribution resulted from injection of 8-cell embryos with embryonic stem cells. The fully agouti colored chimeras were generated from both injection and coculture of 8-cell embryos with embryonic stem cells. Additionally, microsatellite DNA screening showed that the fully agouti colored chimeras were fully embryonic stem cell derived mice. Unlike embryonic stem cells, the mouse chimeras were only generated from injection of 8-cell embryos with induced pluripotent stem cells and none of these showed germline transmission. The results indicated that injection of 8-cell embryos is the most efficient method for assessing stem cell pluripotency and generating induced pluripotent stem cell chimeras, embryonic stem cell chimeras with germline transmission, and fully mouse embryonic stem cell derived mice.http://dx.doi.org/10.1155/2014/409021 |
| spellingShingle | Jitong Guo Baojiang Wu Shuyu Li Siqin Bao Lixia Zhao Shuxiang Hu Wei Sun Jie Su Yanfeng Dai Xihe Li Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos Stem Cells International |
| title | Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos |
| title_full | Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos |
| title_fullStr | Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos |
| title_full_unstemmed | Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos |
| title_short | Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos |
| title_sort | contribution of mouse embryonic stem cells and induced pluripotent stem cells to chimeras through injection and coculture of embryos |
| url | http://dx.doi.org/10.1155/2014/409021 |
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