Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon.

Acute febrile patients presenting at hospitals in Douala, Cameroon between July and December 2020, were screened for dengue infections using real time RT-PCR on fragments of the 5' and 3' UTR genomic regions. In total, 12.8% (41/320) of cases examined were positive for dengue. Dengue virus...

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Main Authors: Huguette Simo Tchetgna, Francine Sado Yousseu, Basile Kamgang, Armel Tedjou, Philip J McCall, Charles S Wondji
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-10-01
Series:PLoS Neglected Tropical Diseases
Online Access:https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0009860&type=printable
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author Huguette Simo Tchetgna
Francine Sado Yousseu
Basile Kamgang
Armel Tedjou
Philip J McCall
Charles S Wondji
author_facet Huguette Simo Tchetgna
Francine Sado Yousseu
Basile Kamgang
Armel Tedjou
Philip J McCall
Charles S Wondji
author_sort Huguette Simo Tchetgna
collection DOAJ
description Acute febrile patients presenting at hospitals in Douala, Cameroon between July and December 2020, were screened for dengue infections using real time RT-PCR on fragments of the 5' and 3' UTR genomic regions. In total, 12.8% (41/320) of cases examined were positive for dengue. Dengue virus 3 (DENV-3) was the most common serotype found (68.3%), followed by DENV-2 (19.5%) and DENV-1 (4.9%). Co-infections of DENV-3 and DENV-2 were found in 3 cases. Jaundice and headache were the most frequent clinical signs associated with infection and 56% (23/41) of the cases were co-infections with malaria. Phylogenetic analysis of the envelope gene identified DENV-1 as belonging to genotype V, DENV-2 to genotype II and DENV-3 to genotype III. The simultaneous occurrence of three serotypes in Douala reveals dengue as a serious public health threat for Cameroon and highlights the need for further epidemiological studies in the major cities of this region.
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institution Kabale University
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publishDate 2021-10-01
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spelling doaj-art-b40ef5f43e4c492a9f03b25e3720f2b32025-08-20T03:25:19ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352021-10-011510e000986010.1371/journal.pntd.0009860Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon.Huguette Simo TchetgnaFrancine Sado YousseuBasile KamgangArmel TedjouPhilip J McCallCharles S WondjiAcute febrile patients presenting at hospitals in Douala, Cameroon between July and December 2020, were screened for dengue infections using real time RT-PCR on fragments of the 5' and 3' UTR genomic regions. In total, 12.8% (41/320) of cases examined were positive for dengue. Dengue virus 3 (DENV-3) was the most common serotype found (68.3%), followed by DENV-2 (19.5%) and DENV-1 (4.9%). Co-infections of DENV-3 and DENV-2 were found in 3 cases. Jaundice and headache were the most frequent clinical signs associated with infection and 56% (23/41) of the cases were co-infections with malaria. Phylogenetic analysis of the envelope gene identified DENV-1 as belonging to genotype V, DENV-2 to genotype II and DENV-3 to genotype III. The simultaneous occurrence of three serotypes in Douala reveals dengue as a serious public health threat for Cameroon and highlights the need for further epidemiological studies in the major cities of this region.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0009860&type=printable
spellingShingle Huguette Simo Tchetgna
Francine Sado Yousseu
Basile Kamgang
Armel Tedjou
Philip J McCall
Charles S Wondji
Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon.
PLoS Neglected Tropical Diseases
title Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon.
title_full Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon.
title_fullStr Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon.
title_full_unstemmed Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon.
title_short Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon.
title_sort concurrent circulation of dengue serotype 1 2 and 3 among acute febrile patients in cameroon
url https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0009860&type=printable
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