A preliminary study of serum metabolomic profiling in male patients with acute brucellosis
Abstract Brucellosis is a significant zoonotic disease that may lead to metabolic profile changes, which remain insufficiently studied. This study utilized an ultra-high performance liquid chromatography coupled with Q Exactive-Orbitrap mass spectrometry (UHPLC-QExactive-Orbitrap MS/MS) to investiga...
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Nature Portfolio
2025-05-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-00661-8 |
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| author | Shenglian Niu Qiang Li Jianling Wang Jiquan Li Yanmei Zhao Hongmei Xue Li Ma Zhijun Zhao Qingwen Zhang |
| author_facet | Shenglian Niu Qiang Li Jianling Wang Jiquan Li Yanmei Zhao Hongmei Xue Li Ma Zhijun Zhao Qingwen Zhang |
| author_sort | Shenglian Niu |
| collection | DOAJ |
| description | Abstract Brucellosis is a significant zoonotic disease that may lead to metabolic profile changes, which remain insufficiently studied. This study utilized an ultra-high performance liquid chromatography coupled with Q Exactive-Orbitrap mass spectrometry (UHPLC-QExactive-Orbitrap MS/MS) to investigate serum samples of acute brucellosis in 32 male patients against 32 well-matched healthy controls. The results revealed nine differential metabolites that correlated with human acute brucellosis, all showing increased levels, except cis-4-hydroxy-D-proline, inosine, hypoxanthine and azelaic acid. These differential metabolites were predominantly involved in metabolic pathways, such as primary bile acid biosynthesis, purine metabolism, taurine and hypotaurine metabolism, and d-amino acid metabolism. This study identified potential metabolite biomarkers of acute brucellosis and laid the foundation for its early diagnosis and prognostic assessment, thus helping to prevent the chronicity of acute brucellosis. |
| format | Article |
| id | doaj-art-b40e79fa7ef1425ebacec8f3e340becb |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-b40e79fa7ef1425ebacec8f3e340becb2025-08-20T03:09:35ZengNature PortfolioScientific Reports2045-23222025-05-0115111610.1038/s41598-025-00661-8A preliminary study of serum metabolomic profiling in male patients with acute brucellosisShenglian Niu0Qiang Li1Jianling Wang2Jiquan Li3Yanmei Zhao4Hongmei Xue5Li Ma6Zhijun Zhao7Qingwen Zhang8Department of Public Health, Qinghai University Medical CollegeQinghai Institute for Endemic Disease Prevention and ControlQinghai Institute for Endemic Disease Prevention and ControlQinghai Institute for Endemic Disease Prevention and ControlQinghai Institute for Endemic Disease Prevention and ControlQinghai Institute for Endemic Disease Prevention and ControlQinghai Institute for Endemic Disease Prevention and ControlQinghai Institute for Endemic Disease Prevention and ControlQinghai Institute for Endemic Disease Prevention and ControlAbstract Brucellosis is a significant zoonotic disease that may lead to metabolic profile changes, which remain insufficiently studied. This study utilized an ultra-high performance liquid chromatography coupled with Q Exactive-Orbitrap mass spectrometry (UHPLC-QExactive-Orbitrap MS/MS) to investigate serum samples of acute brucellosis in 32 male patients against 32 well-matched healthy controls. The results revealed nine differential metabolites that correlated with human acute brucellosis, all showing increased levels, except cis-4-hydroxy-D-proline, inosine, hypoxanthine and azelaic acid. These differential metabolites were predominantly involved in metabolic pathways, such as primary bile acid biosynthesis, purine metabolism, taurine and hypotaurine metabolism, and d-amino acid metabolism. This study identified potential metabolite biomarkers of acute brucellosis and laid the foundation for its early diagnosis and prognostic assessment, thus helping to prevent the chronicity of acute brucellosis.https://doi.org/10.1038/s41598-025-00661-8BrucellosisQinghai ProvinceSerum metabolomicsMetabolites |
| spellingShingle | Shenglian Niu Qiang Li Jianling Wang Jiquan Li Yanmei Zhao Hongmei Xue Li Ma Zhijun Zhao Qingwen Zhang A preliminary study of serum metabolomic profiling in male patients with acute brucellosis Scientific Reports Brucellosis Qinghai Province Serum metabolomics Metabolites |
| title | A preliminary study of serum metabolomic profiling in male patients with acute brucellosis |
| title_full | A preliminary study of serum metabolomic profiling in male patients with acute brucellosis |
| title_fullStr | A preliminary study of serum metabolomic profiling in male patients with acute brucellosis |
| title_full_unstemmed | A preliminary study of serum metabolomic profiling in male patients with acute brucellosis |
| title_short | A preliminary study of serum metabolomic profiling in male patients with acute brucellosis |
| title_sort | preliminary study of serum metabolomic profiling in male patients with acute brucellosis |
| topic | Brucellosis Qinghai Province Serum metabolomics Metabolites |
| url | https://doi.org/10.1038/s41598-025-00661-8 |
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