Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in Drosophila
Background: Cassia siamea L., an angiosperm native to Southeast Asia, has been traditionally used for various medicinal purposes, including antifever, antihypertensive, and antidiabetic applications. Despite its traditional use, limited scientific evidence supports its antiobesity properties. Purpos...
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Elsevier
2025-02-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031325000168 |
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| author | Ananya Dechakhamphu Nattapong Wongchum Theeraphan Chumroenphat Alongklod Tanomtong Sirithon Siriamornpun |
| author_facet | Ananya Dechakhamphu Nattapong Wongchum Theeraphan Chumroenphat Alongklod Tanomtong Sirithon Siriamornpun |
| author_sort | Ananya Dechakhamphu |
| collection | DOAJ |
| description | Background: Cassia siamea L., an angiosperm native to Southeast Asia, has been traditionally used for various medicinal purposes, including antifever, antihypertensive, and antidiabetic applications. Despite its traditional use, limited scientific evidence supports its antiobesity properties. Purpose: This study aimed to investigate the antiobesity effects of C. siamea extract (CSE) both in vitro and in vivo. Methods: CSE was obtained via extraction from C. siamea wood with 70 % ethanol and its phytochemical content was analyzed by gas chromatography–mass spectrometry (GC‒MS) and high-performance liquid chromatography (HPLC). Drosophila was used to examine the effects of supplementation with C. siamea extract on lipase activity, body weight, triglyceride levels, physical strength, survival, and antioxidant activity in Drosophila fed a high-fat diet. Moreover, this study investigated the effects of the extract combined with a high-fat diet on amino acid metabolism and proteomic profiles to clarify its mechanism of action. Results: Syringic acid was the most abundant phenolic compound in CSE, followed by gallic acid, sinapic acid, protocatechuic acid, cinnamic acid, chlorogenic acid, and ferulic acid. In vitro, CSE significantly inhibited pancreatic lipase activity (IC50 = 67.73±3.24 µg/mL) in a noncompetitive manner. In a high-fat diet-fed Drosophila model, CSE supplementation (10 mg/mL) reduced lipase activity and triglyceride levels and improved body weight, suggesting antiobesity and potential antidiabetic effects. Additionally, CSE increased survival rates, physical strength, and antioxidant capacity, as evidenced by increased SOD1 activity and resistance to oxidative stress. CSE helped restore disrupted amino acid levels, particularly glutamine, alanine, and proline, indicating improved metabolic regulation. The results of the quantitative proteomics analysis indicate that a high-fat diet (HFD) alters proteins within metabolic pathways, which can be minimized in severity through CSE therapy. Conclusion: These findings highlight the potential therapeutic role of CSE in managing obesity and related metabolic disorders. |
| format | Article |
| id | doaj-art-b40b1f9b061643839a16807baa47f47e |
| institution | Kabale University |
| issn | 2667-0313 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj-art-b40b1f9b061643839a16807baa47f47e2025-02-10T04:35:23ZengElsevierPhytomedicine Plus2667-03132025-02-0151100743Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in DrosophilaAnanya Dechakhamphu0Nattapong Wongchum1Theeraphan Chumroenphat2Alongklod Tanomtong3Sirithon Siriamornpun4Thai Traditional Medicine Program, Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubonratchathani, 34000, Thailand; Cosmetic Science and Spa Program, Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubonratchathani, 34000, ThailandBiology Program, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubonratchathani, 34000, ThailandThai Traditional Medicine Program, Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubonratchathani, 34000, Thailand; Cosmetic Science and Spa Program, Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubonratchathani, 34000, ThailandBiology Program, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, ThailandResearch Unit of Thai Food Innovation, Department of Food Technology and Nutrition, Mahasarakham University, Kantarawichai, Maha Sarakham, 44150, Thailand; Corresponding author at: Research Unit of Thai Food Innovation, Department of Food Technology and Nutrition, Mahasarakham University, Kantarawichai, Maha Sarakham, 44150, ThailandBackground: Cassia siamea L., an angiosperm native to Southeast Asia, has been traditionally used for various medicinal purposes, including antifever, antihypertensive, and antidiabetic applications. Despite its traditional use, limited scientific evidence supports its antiobesity properties. Purpose: This study aimed to investigate the antiobesity effects of C. siamea extract (CSE) both in vitro and in vivo. Methods: CSE was obtained via extraction from C. siamea wood with 70 % ethanol and its phytochemical content was analyzed by gas chromatography–mass spectrometry (GC‒MS) and high-performance liquid chromatography (HPLC). Drosophila was used to examine the effects of supplementation with C. siamea extract on lipase activity, body weight, triglyceride levels, physical strength, survival, and antioxidant activity in Drosophila fed a high-fat diet. Moreover, this study investigated the effects of the extract combined with a high-fat diet on amino acid metabolism and proteomic profiles to clarify its mechanism of action. Results: Syringic acid was the most abundant phenolic compound in CSE, followed by gallic acid, sinapic acid, protocatechuic acid, cinnamic acid, chlorogenic acid, and ferulic acid. In vitro, CSE significantly inhibited pancreatic lipase activity (IC50 = 67.73±3.24 µg/mL) in a noncompetitive manner. In a high-fat diet-fed Drosophila model, CSE supplementation (10 mg/mL) reduced lipase activity and triglyceride levels and improved body weight, suggesting antiobesity and potential antidiabetic effects. Additionally, CSE increased survival rates, physical strength, and antioxidant capacity, as evidenced by increased SOD1 activity and resistance to oxidative stress. CSE helped restore disrupted amino acid levels, particularly glutamine, alanine, and proline, indicating improved metabolic regulation. The results of the quantitative proteomics analysis indicate that a high-fat diet (HFD) alters proteins within metabolic pathways, which can be minimized in severity through CSE therapy. Conclusion: These findings highlight the potential therapeutic role of CSE in managing obesity and related metabolic disorders.http://www.sciencedirect.com/science/article/pii/S2667031325000168Anti-obesity agentPlant extractBiological productObesityAnimal model |
| spellingShingle | Ananya Dechakhamphu Nattapong Wongchum Theeraphan Chumroenphat Alongklod Tanomtong Sirithon Siriamornpun Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in Drosophila Phytomedicine Plus Anti-obesity agent Plant extract Biological product Obesity Animal model |
| title | Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in Drosophila |
| title_full | Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in Drosophila |
| title_fullStr | Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in Drosophila |
| title_full_unstemmed | Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in Drosophila |
| title_short | Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in Drosophila |
| title_sort | quantitative proteomics and targeted metabolomics reveal the role of cassia siamea l extract in the response to high fat diet induced obesity in drosophila |
| topic | Anti-obesity agent Plant extract Biological product Obesity Animal model |
| url | http://www.sciencedirect.com/science/article/pii/S2667031325000168 |
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