Development, Optimization, and Validation of a Quantitative PCR Assay for <i>Borrelia burgdorferi</i> Detection in Tick, Wildlife, and Human Samples

Development, Optimization, and Validation of a Quantitative PCR Assay for <i>Borrelia burgdorferi</i> Detection in Tick, Wildlife, and Human Samples

Tick-borne pathogens are growing in importance for human and veterinary research worldwide. We developed, optimized, and validated a reliable quantitative PCR (qPCR; real-time PCR) assay to assess Borrelia burgdorferi infection by targeting two B. burgdorferi genes, <i>ospA</i> and <i...

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Bibliographic Details
Main Authors: Julie Lewis, Vett K. Lloyd, Gilles A. Robichaud
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Pathogens
Subjects:
<i>Borrelia burgdorferi</i>
Lyme disease
ticks
wildlife
<i>Borrelia</i> culture
qPCR
Online Access:https://www.mdpi.com/2076-0817/13/12/1034
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