Cytomorphometric analysis of buccal mucosal cells in iron deficiency anemia: A cross-sectional study
Background: Worldwide, at any given moment, more individuals have iron deficiency anemia (IDA) than any other health problems. Very few studies have been conducted to evaluate changes in oral mucosa in IDA. The present study is undertaken for cytomorphometric analysis of buccal mucosal cells in IDA....
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2023-07-01
|
| Series: | Indian Journal of Pathology and Microbiology |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/ijpm.ijpm_43_22 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591256175247360 |
|---|---|
| author | S Patil Swati J Palaskar Sangeeta R Narang Bindiya B Pawar Rasika T Kathuriya Pargatsingh S Khandalkar Yogesh |
| author_facet | S Patil Swati J Palaskar Sangeeta R Narang Bindiya B Pawar Rasika T Kathuriya Pargatsingh S Khandalkar Yogesh |
| author_sort | S Patil Swati |
| collection | DOAJ |
| description | Background:
Worldwide, at any given moment, more individuals have iron deficiency anemia (IDA) than any other health problems. Very few studies have been conducted to evaluate changes in oral mucosa in IDA. The present study is undertaken for cytomorphometric analysis of buccal mucosal cells in IDA. Nuclear diameter (ND), cell diameter (CD), nuclear area (NA), cell area (CA), and nuclear-cytoplasmic ratio (N/C) are measured and compared in buccal mucosal cells of IDA patients and controls.
Aims:
To evaluate cytomorphometric changes in epithelial cells of oral mucosa in IDA and to compare these changes with the controls.
Settings and Design:
A cross-sectional study.
Methods and Materials:
Forty cases of IDA and forty cases of control group were selected for the study. IDA cases were diagnosed with decreased Hb level, MCV, MCH, MCHC and confirmed by decreased serum iron and increased total iron-binding capacity levels. Cytomorphometric analysis of buccal mucosa was performed to study CA, CD, NA, ND, and N/C ratio.
Statistical Analysis Used:
Statistical analysis was done using t- test in SPSS software version 4.
Result:
Results of our study showed increased cell area, cell diameter, nuclear area, nuclear diameter, and nuclear-cytoplasmic ratio in anemic group as compared to controls.
Conclusion:
The study suggests that individuals with IDA regardless of clinically visible oral lesions show cytological changes in oral mucosal epithelium. Knowledge of quantitative alterations in oral epithelial cells of IDA patients is important as these alterations are similar to those seen in precancerous and radiation-induced changes. Cytomorphometry is an efficient tool to understand the extent of cellular changes that occur in oral epithelial cells in IDA. |
| format | Article |
| id | doaj-art-b3fdc591f9a440829ed3219cb5440818 |
| institution | Kabale University |
| issn | 0377-4929 0974-5130 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj-art-b3fdc591f9a440829ed3219cb54408182025-01-22T15:08:58ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49290974-51302023-07-01671566110.4103/ijpm.ijpm_43_22Cytomorphometric analysis of buccal mucosal cells in iron deficiency anemia: A cross-sectional studyS Patil SwatiJ Palaskar SangeetaR Narang BindiyaB Pawar RasikaT Kathuriya PargatsinghS Khandalkar YogeshBackground: Worldwide, at any given moment, more individuals have iron deficiency anemia (IDA) than any other health problems. Very few studies have been conducted to evaluate changes in oral mucosa in IDA. The present study is undertaken for cytomorphometric analysis of buccal mucosal cells in IDA. Nuclear diameter (ND), cell diameter (CD), nuclear area (NA), cell area (CA), and nuclear-cytoplasmic ratio (N/C) are measured and compared in buccal mucosal cells of IDA patients and controls. Aims: To evaluate cytomorphometric changes in epithelial cells of oral mucosa in IDA and to compare these changes with the controls. Settings and Design: A cross-sectional study. Methods and Materials: Forty cases of IDA and forty cases of control group were selected for the study. IDA cases were diagnosed with decreased Hb level, MCV, MCH, MCHC and confirmed by decreased serum iron and increased total iron-binding capacity levels. Cytomorphometric analysis of buccal mucosa was performed to study CA, CD, NA, ND, and N/C ratio. Statistical Analysis Used: Statistical analysis was done using t- test in SPSS software version 4. Result: Results of our study showed increased cell area, cell diameter, nuclear area, nuclear diameter, and nuclear-cytoplasmic ratio in anemic group as compared to controls. Conclusion: The study suggests that individuals with IDA regardless of clinically visible oral lesions show cytological changes in oral mucosal epithelium. Knowledge of quantitative alterations in oral epithelial cells of IDA patients is important as these alterations are similar to those seen in precancerous and radiation-induced changes. Cytomorphometry is an efficient tool to understand the extent of cellular changes that occur in oral epithelial cells in IDA.https://journals.lww.com/10.4103/ijpm.ijpm_43_22buccal mucosacytomorphometryiron deficiency anemia |
| spellingShingle | S Patil Swati J Palaskar Sangeeta R Narang Bindiya B Pawar Rasika T Kathuriya Pargatsingh S Khandalkar Yogesh Cytomorphometric analysis of buccal mucosal cells in iron deficiency anemia: A cross-sectional study Indian Journal of Pathology and Microbiology buccal mucosa cytomorphometry iron deficiency anemia |
| title | Cytomorphometric analysis of buccal mucosal cells in iron deficiency anemia: A cross-sectional study |
| title_full | Cytomorphometric analysis of buccal mucosal cells in iron deficiency anemia: A cross-sectional study |
| title_fullStr | Cytomorphometric analysis of buccal mucosal cells in iron deficiency anemia: A cross-sectional study |
| title_full_unstemmed | Cytomorphometric analysis of buccal mucosal cells in iron deficiency anemia: A cross-sectional study |
| title_short | Cytomorphometric analysis of buccal mucosal cells in iron deficiency anemia: A cross-sectional study |
| title_sort | cytomorphometric analysis of buccal mucosal cells in iron deficiency anemia a cross sectional study |
| topic | buccal mucosa cytomorphometry iron deficiency anemia |
| url | https://journals.lww.com/10.4103/ijpm.ijpm_43_22 |
| work_keys_str_mv | AT spatilswati cytomorphometricanalysisofbuccalmucosalcellsinirondeficiencyanemiaacrosssectionalstudy AT jpalaskarsangeeta cytomorphometricanalysisofbuccalmucosalcellsinirondeficiencyanemiaacrosssectionalstudy AT rnarangbindiya cytomorphometricanalysisofbuccalmucosalcellsinirondeficiencyanemiaacrosssectionalstudy AT bpawarrasika cytomorphometricanalysisofbuccalmucosalcellsinirondeficiencyanemiaacrosssectionalstudy AT tkathuriyapargatsingh cytomorphometricanalysisofbuccalmucosalcellsinirondeficiencyanemiaacrosssectionalstudy AT skhandalkaryogesh cytomorphometricanalysisofbuccalmucosalcellsinirondeficiencyanemiaacrosssectionalstudy |