Modification of <i>Saccharomyces cerevisiae</i> Cells with Metal Hexacyanoferrates for the Construction of a Yeast-Based Fuel Cell
This research presents a simple procedure for chemically modifying yeast (<i>Saccharomyces cerevisiae</i>) cells with nickel hexacyanoferrate (NiHCF) and ferric hexacyanoferrate, also known as Prussian blue (PB), to increase the conductivity of the yeast cell wall. Using linear sweep vol...
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2025-01-01
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| author | Gabija Adomaitė Povilas Virbickas Aušra Valiūnienė |
| author_facet | Gabija Adomaitė Povilas Virbickas Aušra Valiūnienė |
| author_sort | Gabija Adomaitė |
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| description | This research presents a simple procedure for chemically modifying yeast (<i>Saccharomyces cerevisiae</i>) cells with nickel hexacyanoferrate (NiHCF) and ferric hexacyanoferrate, also known as Prussian blue (PB), to increase the conductivity of the yeast cell wall. Using linear sweep voltammetry, NiHCF-modified yeast and PB-modified yeast (NiHCF/yeast and PB/yeast, respectively) were found to have better cell wall conductivity in [Fe(CN)<sub>6</sub>]<sup>3−</sup> and glucose-containing phosphate-buffered solution than unmodified yeast. Spectrophotometric analysis showed that the modification of yeast cells with NiHCF had a less harmful effect on yeast cell viability than the modification of yeast cells with PB. The use of NiHCF/yeast and PB/yeast cells in the construction of a yeast-based fuel cell allowed the maximum power densities of 62.66 mW/m<sup>2</sup> and 94.09 mW/m<sup>2</sup> to be achieved. These values were much higher than those obtained using unmodified yeast cells (42.25 mW/m<sup>2</sup>). NiHCF/yeast and PB/yeast fuel cells were renewed by replenishing the yeast suspension in the anolyte or the FeCl<sub>3</sub> salt in the catholyte. This allowed 77.4% and 50.1% of the initial maximum power density of the fuel cells to be achieved. |
| format | Article |
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| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Molecules |
| spelling | doaj-art-b3f9528de7784c3ba0bea46ca5fef6542025-01-10T13:18:59ZengMDPI AGMolecules1420-30492025-01-0130113710.3390/molecules30010137Modification of <i>Saccharomyces cerevisiae</i> Cells with Metal Hexacyanoferrates for the Construction of a Yeast-Based Fuel CellGabija Adomaitė0Povilas Virbickas1Aušra Valiūnienė2Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, LithuaniaInstitute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, LithuaniaInstitute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, LithuaniaThis research presents a simple procedure for chemically modifying yeast (<i>Saccharomyces cerevisiae</i>) cells with nickel hexacyanoferrate (NiHCF) and ferric hexacyanoferrate, also known as Prussian blue (PB), to increase the conductivity of the yeast cell wall. Using linear sweep voltammetry, NiHCF-modified yeast and PB-modified yeast (NiHCF/yeast and PB/yeast, respectively) were found to have better cell wall conductivity in [Fe(CN)<sub>6</sub>]<sup>3−</sup> and glucose-containing phosphate-buffered solution than unmodified yeast. Spectrophotometric analysis showed that the modification of yeast cells with NiHCF had a less harmful effect on yeast cell viability than the modification of yeast cells with PB. The use of NiHCF/yeast and PB/yeast cells in the construction of a yeast-based fuel cell allowed the maximum power densities of 62.66 mW/m<sup>2</sup> and 94.09 mW/m<sup>2</sup> to be achieved. These values were much higher than those obtained using unmodified yeast cells (42.25 mW/m<sup>2</sup>). NiHCF/yeast and PB/yeast fuel cells were renewed by replenishing the yeast suspension in the anolyte or the FeCl<sub>3</sub> salt in the catholyte. This allowed 77.4% and 50.1% of the initial maximum power density of the fuel cells to be achieved.https://www.mdpi.com/1420-3049/30/1/137Prussian bluenickel hexacyanoferratebiofuel cellyeast cells<i>Saccharomyces cerevisiae</i> |
| spellingShingle | Gabija Adomaitė Povilas Virbickas Aušra Valiūnienė Modification of <i>Saccharomyces cerevisiae</i> Cells with Metal Hexacyanoferrates for the Construction of a Yeast-Based Fuel Cell Molecules Prussian blue nickel hexacyanoferrate biofuel cell yeast cells <i>Saccharomyces cerevisiae</i> |
| title | Modification of <i>Saccharomyces cerevisiae</i> Cells with Metal Hexacyanoferrates for the Construction of a Yeast-Based Fuel Cell |
| title_full | Modification of <i>Saccharomyces cerevisiae</i> Cells with Metal Hexacyanoferrates for the Construction of a Yeast-Based Fuel Cell |
| title_fullStr | Modification of <i>Saccharomyces cerevisiae</i> Cells with Metal Hexacyanoferrates for the Construction of a Yeast-Based Fuel Cell |
| title_full_unstemmed | Modification of <i>Saccharomyces cerevisiae</i> Cells with Metal Hexacyanoferrates for the Construction of a Yeast-Based Fuel Cell |
| title_short | Modification of <i>Saccharomyces cerevisiae</i> Cells with Metal Hexacyanoferrates for the Construction of a Yeast-Based Fuel Cell |
| title_sort | modification of i saccharomyces cerevisiae i cells with metal hexacyanoferrates for the construction of a yeast based fuel cell |
| topic | Prussian blue nickel hexacyanoferrate biofuel cell yeast cells <i>Saccharomyces cerevisiae</i> |
| url | https://www.mdpi.com/1420-3049/30/1/137 |
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