Ultra-Sensitive Detection of Chloramphenicol by CdS@NiMoS Nanorods-Based Photoelectrochemical Aptasensor

A novel nanomaterial photoelectrochemical aptamer sensor based on CdS@NiMoS heterojunction nanocomposites was constructed for highly sensitive detection of chloramphenicol (CAP) in antibiotic residues. Through optimization of the material synthesis process, the optimal doping ratio of MoS<sub>...

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Main Authors: Hebin Sun, Yimeng Sun, Tong Qi, Zhenyu Wang, Jianlong Zhao, Lijuan Liang
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Biosensors
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Online Access:https://www.mdpi.com/2079-6374/15/7/454
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author Hebin Sun
Yimeng Sun
Tong Qi
Zhenyu Wang
Jianlong Zhao
Lijuan Liang
author_facet Hebin Sun
Yimeng Sun
Tong Qi
Zhenyu Wang
Jianlong Zhao
Lijuan Liang
author_sort Hebin Sun
collection DOAJ
description A novel nanomaterial photoelectrochemical aptamer sensor based on CdS@NiMoS heterojunction nanocomposites was constructed for highly sensitive detection of chloramphenicol (CAP) in antibiotic residues. Through optimization of the material synthesis process, the optimal doping ratio of MoS<sub>2</sub> to Ni<sup>3+</sup> (70% MoS<sub>2</sub> and 10% Ni<sup>3+</sup>) was identified, which significantly enhanced the photogenerated carrier separation efficiency. In thin-film preparation, comparative analysis of four film-forming methods led to the determination of an optimal process with stability. To achieve highly specific CAP detection, the nanocomposite chip was integrated with nucleic acid aptamer biorecognition elements within a standard three-electrode detection system. Experimental results demonstrated a linear response (R<sup>2</sup> = 0.998) in the 0.1–2 μM concentration range, with a detection limit of 3.69 nM (3σ/S).
format Article
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institution DOAJ
issn 2079-6374
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publishDate 2025-07-01
publisher MDPI AG
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series Biosensors
spelling doaj-art-b3f89bf711dc4cf9a21782bd450f893b2025-08-20T02:45:34ZengMDPI AGBiosensors2079-63742025-07-0115745410.3390/bios15070454Ultra-Sensitive Detection of Chloramphenicol by CdS@NiMoS Nanorods-Based Photoelectrochemical AptasensorHebin Sun0Yimeng Sun1Tong Qi2Zhenyu Wang3Jianlong Zhao4Lijuan Liang5State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaSchool of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, ChinaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaA novel nanomaterial photoelectrochemical aptamer sensor based on CdS@NiMoS heterojunction nanocomposites was constructed for highly sensitive detection of chloramphenicol (CAP) in antibiotic residues. Through optimization of the material synthesis process, the optimal doping ratio of MoS<sub>2</sub> to Ni<sup>3+</sup> (70% MoS<sub>2</sub> and 10% Ni<sup>3+</sup>) was identified, which significantly enhanced the photogenerated carrier separation efficiency. In thin-film preparation, comparative analysis of four film-forming methods led to the determination of an optimal process with stability. To achieve highly specific CAP detection, the nanocomposite chip was integrated with nucleic acid aptamer biorecognition elements within a standard three-electrode detection system. Experimental results demonstrated a linear response (R<sup>2</sup> = 0.998) in the 0.1–2 μM concentration range, with a detection limit of 3.69 nM (3σ/S).https://www.mdpi.com/2079-6374/15/7/454CdS@NiMoSchloramphenicolaptsensorPEC
spellingShingle Hebin Sun
Yimeng Sun
Tong Qi
Zhenyu Wang
Jianlong Zhao
Lijuan Liang
Ultra-Sensitive Detection of Chloramphenicol by CdS@NiMoS Nanorods-Based Photoelectrochemical Aptasensor
Biosensors
CdS@NiMoS
chloramphenicol
aptsensor
PEC
title Ultra-Sensitive Detection of Chloramphenicol by CdS@NiMoS Nanorods-Based Photoelectrochemical Aptasensor
title_full Ultra-Sensitive Detection of Chloramphenicol by CdS@NiMoS Nanorods-Based Photoelectrochemical Aptasensor
title_fullStr Ultra-Sensitive Detection of Chloramphenicol by CdS@NiMoS Nanorods-Based Photoelectrochemical Aptasensor
title_full_unstemmed Ultra-Sensitive Detection of Chloramphenicol by CdS@NiMoS Nanorods-Based Photoelectrochemical Aptasensor
title_short Ultra-Sensitive Detection of Chloramphenicol by CdS@NiMoS Nanorods-Based Photoelectrochemical Aptasensor
title_sort ultra sensitive detection of chloramphenicol by cds nimos nanorods based photoelectrochemical aptasensor
topic CdS@NiMoS
chloramphenicol
aptsensor
PEC
url https://www.mdpi.com/2079-6374/15/7/454
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AT yimengsun ultrasensitivedetectionofchloramphenicolbycdsnimosnanorodsbasedphotoelectrochemicalaptasensor
AT tongqi ultrasensitivedetectionofchloramphenicolbycdsnimosnanorodsbasedphotoelectrochemicalaptasensor
AT zhenyuwang ultrasensitivedetectionofchloramphenicolbycdsnimosnanorodsbasedphotoelectrochemicalaptasensor
AT jianlongzhao ultrasensitivedetectionofchloramphenicolbycdsnimosnanorodsbasedphotoelectrochemicalaptasensor
AT lijuanliang ultrasensitivedetectionofchloramphenicolbycdsnimosnanorodsbasedphotoelectrochemicalaptasensor