Ultra-Sensitive Detection of Chloramphenicol by CdS@NiMoS Nanorods-Based Photoelectrochemical Aptasensor

A novel nanomaterial photoelectrochemical aptamer sensor based on CdS@NiMoS heterojunction nanocomposites was constructed for highly sensitive detection of chloramphenicol (CAP) in antibiotic residues. Through optimization of the material synthesis process, the optimal doping ratio of MoS<sub>...

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Bibliographic Details
Main Authors: Hebin Sun, Yimeng Sun, Tong Qi, Zhenyu Wang, Jianlong Zhao, Lijuan Liang
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Biosensors
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Online Access:https://www.mdpi.com/2079-6374/15/7/454
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Summary:A novel nanomaterial photoelectrochemical aptamer sensor based on CdS@NiMoS heterojunction nanocomposites was constructed for highly sensitive detection of chloramphenicol (CAP) in antibiotic residues. Through optimization of the material synthesis process, the optimal doping ratio of MoS<sub>2</sub> to Ni<sup>3+</sup> (70% MoS<sub>2</sub> and 10% Ni<sup>3+</sup>) was identified, which significantly enhanced the photogenerated carrier separation efficiency. In thin-film preparation, comparative analysis of four film-forming methods led to the determination of an optimal process with stability. To achieve highly specific CAP detection, the nanocomposite chip was integrated with nucleic acid aptamer biorecognition elements within a standard three-electrode detection system. Experimental results demonstrated a linear response (R<sup>2</sup> = 0.998) in the 0.1–2 μM concentration range, with a detection limit of 3.69 nM (3σ/S).
ISSN:2079-6374