Exploring the diversity of galls on Artemisia indica induced by Rhopalomyia species through morphological and transcriptome analyses
Abstract Plant galls generated by insects have highly organized structures, providing nutrients and shelter to the insects living within them. Most research on the physiological and molecular mechanisms of gall development has focused on single galls. To understand the diversity of gall development,...
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| Format: | Article |
| Language: | English |
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Wiley
2024-07-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.619 |
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| _version_ | 1841558653352542208 |
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| author | Seiji Takeda Makiko Yoza Sawako Ueda Sakura Takeuchi Akiteru Maeno Tomoaki Sakamoto Seisuke Kimura |
| author_facet | Seiji Takeda Makiko Yoza Sawako Ueda Sakura Takeuchi Akiteru Maeno Tomoaki Sakamoto Seisuke Kimura |
| author_sort | Seiji Takeda |
| collection | DOAJ |
| description | Abstract Plant galls generated by insects have highly organized structures, providing nutrients and shelter to the insects living within them. Most research on the physiological and molecular mechanisms of gall development has focused on single galls. To understand the diversity of gall development, we examined five galls with different morphologies generated by distinct species of Rhopalomyia (gall midge; Diptera: Cecidomyiidae) on a single host plant of Artemisia indica var. maximowiczii (Asteraceae). Vasculature developed de novo within the galls, indicating active transport of nutrients between galls and the host plant. Each gall had a different pattern of vasculature and lignification, probably due to differences in the site of gall generation and the gall midge species. Transcriptome analysis indicated that photosynthetic and cell wall–related genes were down‐regulated in leaf and stem galls, respectively, compared with control leaf and stem tissues, whereas genes involved in floral organ development were up‐regulated in all types of galls, indicating that transformation from source to sink organs occurs during gall development. Our results help to understand the diversity of galls on a single herbaceous host plant. |
| format | Article |
| id | doaj-art-b3f15393e1974e4689a014f6cd55407d |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-b3f15393e1974e4689a014f6cd55407d2025-01-06T07:21:54ZengWileyPlant Direct2475-44552024-07-0187n/an/a10.1002/pld3.619Exploring the diversity of galls on Artemisia indica induced by Rhopalomyia species through morphological and transcriptome analysesSeiji Takeda0Makiko Yoza1Sawako Ueda2Sakura Takeuchi3Akiteru Maeno4Tomoaki Sakamoto5Seisuke Kimura6Graduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto JapanGraduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto JapanGraduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto JapanGraduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto JapanCell Architecture Laboratory National Institute of Genetics Shizuoka JapanCenter for Plant Sciences Kyoto Sangyo University Kyoto JapanCenter for Plant Sciences Kyoto Sangyo University Kyoto JapanAbstract Plant galls generated by insects have highly organized structures, providing nutrients and shelter to the insects living within them. Most research on the physiological and molecular mechanisms of gall development has focused on single galls. To understand the diversity of gall development, we examined five galls with different morphologies generated by distinct species of Rhopalomyia (gall midge; Diptera: Cecidomyiidae) on a single host plant of Artemisia indica var. maximowiczii (Asteraceae). Vasculature developed de novo within the galls, indicating active transport of nutrients between galls and the host plant. Each gall had a different pattern of vasculature and lignification, probably due to differences in the site of gall generation and the gall midge species. Transcriptome analysis indicated that photosynthetic and cell wall–related genes were down‐regulated in leaf and stem galls, respectively, compared with control leaf and stem tissues, whereas genes involved in floral organ development were up‐regulated in all types of galls, indicating that transformation from source to sink organs occurs during gall development. Our results help to understand the diversity of galls on a single herbaceous host plant.https://doi.org/10.1002/pld3.619Artemisia indicagallmicroCTRhopalomyiaRNA sequencing |
| spellingShingle | Seiji Takeda Makiko Yoza Sawako Ueda Sakura Takeuchi Akiteru Maeno Tomoaki Sakamoto Seisuke Kimura Exploring the diversity of galls on Artemisia indica induced by Rhopalomyia species through morphological and transcriptome analyses Plant Direct Artemisia indica gall microCT Rhopalomyia RNA sequencing |
| title | Exploring the diversity of galls on Artemisia indica induced by Rhopalomyia species through morphological and transcriptome analyses |
| title_full | Exploring the diversity of galls on Artemisia indica induced by Rhopalomyia species through morphological and transcriptome analyses |
| title_fullStr | Exploring the diversity of galls on Artemisia indica induced by Rhopalomyia species through morphological and transcriptome analyses |
| title_full_unstemmed | Exploring the diversity of galls on Artemisia indica induced by Rhopalomyia species through morphological and transcriptome analyses |
| title_short | Exploring the diversity of galls on Artemisia indica induced by Rhopalomyia species through morphological and transcriptome analyses |
| title_sort | exploring the diversity of galls on artemisia indica induced by rhopalomyia species through morphological and transcriptome analyses |
| topic | Artemisia indica gall microCT Rhopalomyia RNA sequencing |
| url | https://doi.org/10.1002/pld3.619 |
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