Fingerprinting of Bacterial Genomes by Amplification of DNA Fragments Surrounding Rare Restriction Sites
A method for generating limited representations of total bacterial DNA, without prior knowledge of the DNA sequence, has been developed. This method consists of three steps: digestion with two restriction enzymes, ligation of two oligonucleotide adapters corresponding to the restriction sites, and s...
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| Main Authors: | Aleksander Masny, Andrzej Plucienniczak |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-10-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01314rr04 |
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