Fingerprinting of Bacterial Genomes by Amplification of DNA Fragments Surrounding Rare Restriction Sites

A method for generating limited representations of total bacterial DNA, without prior knowledge of the DNA sequence, has been developed. This method consists of three steps: digestion with two restriction enzymes, ligation of two oligonucleotide adapters corresponding to the restriction sites, and s...

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Bibliographic Details
Main Authors: Aleksander Masny, Andrzej Plucienniczak
Format: Article
Language:English
Published: Taylor & Francis Group 2001-10-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/01314rr04
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