Gene Methylation as a Potential Epigenetic Marker for Ovarian Cancer
Background: Ovarian cancer (OC) is a significant health problem often diagnosed at an advanced stage due to the lack of early symptoms and effective screening methods. This study aimed to explore the role of CYB5R4 gene methylation as a potential biomarker for ovarian cancer. Methods: DNA isolation...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-06-01
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| Series: | Clinical Medicine Insights: Oncology |
| Online Access: | https://doi.org/10.1177/11795549251340531 |
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| author | Aysegul Gonc Ozge Sukruoglu Erdogan Seda Kilic Erciyas Betul Celik Demirbas Ahmet Dinc Ozge Pasin Pınar Saip Hulya Yazici Seref Bugra Tuncer |
| author_facet | Aysegul Gonc Ozge Sukruoglu Erdogan Seda Kilic Erciyas Betul Celik Demirbas Ahmet Dinc Ozge Pasin Pınar Saip Hulya Yazici Seref Bugra Tuncer |
| author_sort | Aysegul Gonc |
| collection | DOAJ |
| description | Background: Ovarian cancer (OC) is a significant health problem often diagnosed at an advanced stage due to the lack of early symptoms and effective screening methods. This study aimed to explore the role of CYB5R4 gene methylation as a potential biomarker for ovarian cancer. Methods: DNA isolation was performed in the blood samples of 387 ovarian cancer patients, 50 individuals with benign ovarian diseases, and 100 healthy controls. The CYB5R4 gene methylation status was evaluated using the Methyl-Specific Restriction Enzymes (MSREs) technique and methylation levels were compared between the groups. Results: Ovarian cancer patients exhibited the highest mean methylation percentage (9.45%) and median (6.23%), followed by healthy controls with a mean of 9.14% and a median value of 4.47%. Statistical analysis showed significant differences in methylation levels ( P = .041), suggesting that CYB5R4 methylation may be associated with ovarian cancer progression. Conclusion: The CYB5R4 gene methylation may serve as a potential biomarker for ovarian cancer, particularly in distinguishing between malignant and benign conditions. Further research is needed to validate these findings and explore the clinical utility of CYB5R4 methylation in ovarian cancer management. |
| format | Article |
| id | doaj-art-b3ee888fdb594e56a007da8e01e3f409 |
| institution | OA Journals |
| issn | 1179-5549 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Clinical Medicine Insights: Oncology |
| spelling | doaj-art-b3ee888fdb594e56a007da8e01e3f4092025-08-20T02:10:12ZengSAGE PublishingClinical Medicine Insights: Oncology1179-55492025-06-011910.1177/11795549251340531 Gene Methylation as a Potential Epigenetic Marker for Ovarian CancerAysegul Gonc0Ozge Sukruoglu Erdogan1Seda Kilic Erciyas2Betul Celik Demirbas3Ahmet Dinc4Ozge Pasin5Pınar Saip6Hulya Yazici7Seref Bugra Tuncer8Institute of Graduate Studies in Health Sciences, Istanbul University, Istanbul, TürkiyeDepartment of Cancer Genetics, Oncology Institute, Istanbul University, Istanbul, TürkiyeDepartment of Cancer Genetics, Oncology Institute, Istanbul University, Istanbul, TürkiyeDepartment of Cancer Genetics, Oncology Institute, Istanbul University, Istanbul, TürkiyeInstitute of Graduate Studies in Health Sciences, Istanbul University, Istanbul, TürkiyeDepartment of Biostatistics and Medical Informatics, Hamidiye Faculty of Medicine, University of Health Sciences, Istanbul, TürkiyeDepartment of Medical Oncology, Oncology Institute, Istanbul University, Istanbul, TürkiyeMedical Biology and Genetics Department, Medical Faculty, Istanbul Health and Technology University, Istanbul, TürkiyeDepartment of Cancer Genetics, Oncology Institute, Istanbul University, Istanbul, TürkiyeBackground: Ovarian cancer (OC) is a significant health problem often diagnosed at an advanced stage due to the lack of early symptoms and effective screening methods. This study aimed to explore the role of CYB5R4 gene methylation as a potential biomarker for ovarian cancer. Methods: DNA isolation was performed in the blood samples of 387 ovarian cancer patients, 50 individuals with benign ovarian diseases, and 100 healthy controls. The CYB5R4 gene methylation status was evaluated using the Methyl-Specific Restriction Enzymes (MSREs) technique and methylation levels were compared between the groups. Results: Ovarian cancer patients exhibited the highest mean methylation percentage (9.45%) and median (6.23%), followed by healthy controls with a mean of 9.14% and a median value of 4.47%. Statistical analysis showed significant differences in methylation levels ( P = .041), suggesting that CYB5R4 methylation may be associated with ovarian cancer progression. Conclusion: The CYB5R4 gene methylation may serve as a potential biomarker for ovarian cancer, particularly in distinguishing between malignant and benign conditions. Further research is needed to validate these findings and explore the clinical utility of CYB5R4 methylation in ovarian cancer management.https://doi.org/10.1177/11795549251340531 |
| spellingShingle | Aysegul Gonc Ozge Sukruoglu Erdogan Seda Kilic Erciyas Betul Celik Demirbas Ahmet Dinc Ozge Pasin Pınar Saip Hulya Yazici Seref Bugra Tuncer Gene Methylation as a Potential Epigenetic Marker for Ovarian Cancer Clinical Medicine Insights: Oncology |
| title | Gene Methylation as a Potential Epigenetic Marker for Ovarian Cancer |
| title_full | Gene Methylation as a Potential Epigenetic Marker for Ovarian Cancer |
| title_fullStr | Gene Methylation as a Potential Epigenetic Marker for Ovarian Cancer |
| title_full_unstemmed | Gene Methylation as a Potential Epigenetic Marker for Ovarian Cancer |
| title_short | Gene Methylation as a Potential Epigenetic Marker for Ovarian Cancer |
| title_sort | gene methylation as a potential epigenetic marker for ovarian cancer |
| url | https://doi.org/10.1177/11795549251340531 |
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