Corchorus olitorius exhibits antiproliferative potential supported by metabolic profiling and integrative biological analyses

Abstract Herbal nutraceuticals could be employed as alternative or complementary routes for alleviating cancer. Corchorus olitorius (Malvaceae) was employed traditionally in the management of tumors. The study aimed to investigate the antiproliferative activity of C. olitorius leaves. In vitro cytot...

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Main Authors: Salma Sameh, Maha R. A. Abdollah, Ahmed M. Elissawy, Eman Al-Sayed, Rola M. Labib, Lan Ye, Fang-Rong Chang, Abdel Nasser B. Singab
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-02717-1
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author Salma Sameh
Maha R. A. Abdollah
Ahmed M. Elissawy
Eman Al-Sayed
Rola M. Labib
Lan Ye
Fang-Rong Chang
Abdel Nasser B. Singab
author_facet Salma Sameh
Maha R. A. Abdollah
Ahmed M. Elissawy
Eman Al-Sayed
Rola M. Labib
Lan Ye
Fang-Rong Chang
Abdel Nasser B. Singab
author_sort Salma Sameh
collection DOAJ
description Abstract Herbal nutraceuticals could be employed as alternative or complementary routes for alleviating cancer. Corchorus olitorius (Malvaceae) was employed traditionally in the management of tumors. The study aimed to investigate the antiproliferative activity of C. olitorius leaves. In vitro cytotoxic and anti-angiogenic activities of C. olitorius were estimated. The bioactive fraction was subjected to in vivo study on BALB/ c female mice using Ehrlich Ascites Carcinoma model. UPLC-ESI-MS/MS analysis was done to determine the phytometabolites followed by in silico studies on the major identified compounds. The bioactive fraction possessed potent in vitro activity against A549 cells with IC50 = 7.8 µg/mL and exhibited strong anti-angiogenic activity. The in vivo study revealed the safety of the fraction and confirmed its anticancer activity. The tumor volume in the fraction treated group was reduced by 33.7% compared to the control group. UPLC-ESI-MS/MS analysis led to the identification of 25 compounds belonging to different chemical classes. The in silico pharmacodynamic profile revealed that the compounds exhibited agreeable binding affinities toward EGFR, CDK2 and VEGF-A comparable to the standard drugs. C. olitorius is a promising herbal nutraceutical from which effective chemopreventive and anticancer formulations can be developed following further in depth studies.
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spelling doaj-art-b3e94a03310a46d58f2aec1408913dba2025-08-20T02:29:45ZengNature PortfolioScientific Reports2045-23222025-05-0115111510.1038/s41598-025-02717-1Corchorus olitorius exhibits antiproliferative potential supported by metabolic profiling and integrative biological analysesSalma Sameh0Maha R. A. Abdollah1Ahmed M. Elissawy2Eman Al-Sayed3Rola M. Labib4Lan Ye5Fang-Rong Chang6Abdel Nasser B. Singab7Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams UniversityDepartment of Pharmacology, Faculty of Pharmacy, The British University in EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Ain-Shams UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Ain-Shams UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Ain-Shams UniversityCancer Centre, the Second Hospital of Shandong UniversitySchool of Pharmacy and Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Ain-Shams UniversityAbstract Herbal nutraceuticals could be employed as alternative or complementary routes for alleviating cancer. Corchorus olitorius (Malvaceae) was employed traditionally in the management of tumors. The study aimed to investigate the antiproliferative activity of C. olitorius leaves. In vitro cytotoxic and anti-angiogenic activities of C. olitorius were estimated. The bioactive fraction was subjected to in vivo study on BALB/ c female mice using Ehrlich Ascites Carcinoma model. UPLC-ESI-MS/MS analysis was done to determine the phytometabolites followed by in silico studies on the major identified compounds. The bioactive fraction possessed potent in vitro activity against A549 cells with IC50 = 7.8 µg/mL and exhibited strong anti-angiogenic activity. The in vivo study revealed the safety of the fraction and confirmed its anticancer activity. The tumor volume in the fraction treated group was reduced by 33.7% compared to the control group. UPLC-ESI-MS/MS analysis led to the identification of 25 compounds belonging to different chemical classes. The in silico pharmacodynamic profile revealed that the compounds exhibited agreeable binding affinities toward EGFR, CDK2 and VEGF-A comparable to the standard drugs. C. olitorius is a promising herbal nutraceutical from which effective chemopreventive and anticancer formulations can be developed following further in depth studies.https://doi.org/10.1038/s41598-025-02717-1Herbal nutraceuticalsCorchorusMalvaceaeAntiproliferativeAnti-angiogenicUPLC-ESI-MS/MS
spellingShingle Salma Sameh
Maha R. A. Abdollah
Ahmed M. Elissawy
Eman Al-Sayed
Rola M. Labib
Lan Ye
Fang-Rong Chang
Abdel Nasser B. Singab
Corchorus olitorius exhibits antiproliferative potential supported by metabolic profiling and integrative biological analyses
Scientific Reports
Herbal nutraceuticals
Corchorus
Malvaceae
Antiproliferative
Anti-angiogenic
UPLC-ESI-MS/MS
title Corchorus olitorius exhibits antiproliferative potential supported by metabolic profiling and integrative biological analyses
title_full Corchorus olitorius exhibits antiproliferative potential supported by metabolic profiling and integrative biological analyses
title_fullStr Corchorus olitorius exhibits antiproliferative potential supported by metabolic profiling and integrative biological analyses
title_full_unstemmed Corchorus olitorius exhibits antiproliferative potential supported by metabolic profiling and integrative biological analyses
title_short Corchorus olitorius exhibits antiproliferative potential supported by metabolic profiling and integrative biological analyses
title_sort corchorus olitorius exhibits antiproliferative potential supported by metabolic profiling and integrative biological analyses
topic Herbal nutraceuticals
Corchorus
Malvaceae
Antiproliferative
Anti-angiogenic
UPLC-ESI-MS/MS
url https://doi.org/10.1038/s41598-025-02717-1
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