Transcriptomic profiling of blood from autoimmune hepatitis patients reveals potential mechanisms with implications for management.
Autoimmune hepatitis (AIH) is a poorly understood, chronic disease, for which corticosteroids are still the mainstay of therapy and most patients undergo liver biopsy to obtain a diagnosis. We aimed to determine if there was a transcriptomic signature of AIH in the peripheral blood and investigate u...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0264307&type=printable |
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| author | Michele May-Sien Tana Arielle Klepper Amy Lyden Angela Oliveira Pisco Maira Phelps Breann McGee Kelsey Green Sandy Feng Joseph DeRisi Emily Dawn Crawford Craig S Lammert |
| author_facet | Michele May-Sien Tana Arielle Klepper Amy Lyden Angela Oliveira Pisco Maira Phelps Breann McGee Kelsey Green Sandy Feng Joseph DeRisi Emily Dawn Crawford Craig S Lammert |
| author_sort | Michele May-Sien Tana |
| collection | DOAJ |
| description | Autoimmune hepatitis (AIH) is a poorly understood, chronic disease, for which corticosteroids are still the mainstay of therapy and most patients undergo liver biopsy to obtain a diagnosis. We aimed to determine if there was a transcriptomic signature of AIH in the peripheral blood and investigate underlying biologic pathways revealed by gene expression analysis. Whole blood RNA from 75 AIH patients and 25 healthy volunteers was extracted and sequenced. Differential gene expression analysis revealed 249 genes that were significantly differentially expressed in AIH patients compared to controls. Using a random forest algorithm, we determined that less than 10 genes were sufficient to differentiate the two groups in our cohort. Interferon signaling was more active in AIH samples compared to controls, regardless of treatment status. Pegivirus sequences were detected in five AIH samples and 1 healthy sample. The gene expression data and clinical metadata were used to determine 12 genes that were significantly associated with advanced fibrosis in AIH. AIH patients with a partial response to therapy demonstrated decreased evidence of a CD8+ T cell gene expression signal. These findings represent progress in understanding a disease in need of better tests, therapies, and biomarkers. |
| format | Article |
| id | doaj-art-b3e90cb41e634ac896060b18bbbe5c0d |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b3e90cb41e634ac896060b18bbbe5c0d2025-08-20T02:46:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01173e026430710.1371/journal.pone.0264307Transcriptomic profiling of blood from autoimmune hepatitis patients reveals potential mechanisms with implications for management.Michele May-Sien TanaArielle KlepperAmy LydenAngela Oliveira PiscoMaira PhelpsBreann McGeeKelsey GreenSandy FengJoseph DeRisiEmily Dawn CrawfordCraig S LammertAutoimmune hepatitis (AIH) is a poorly understood, chronic disease, for which corticosteroids are still the mainstay of therapy and most patients undergo liver biopsy to obtain a diagnosis. We aimed to determine if there was a transcriptomic signature of AIH in the peripheral blood and investigate underlying biologic pathways revealed by gene expression analysis. Whole blood RNA from 75 AIH patients and 25 healthy volunteers was extracted and sequenced. Differential gene expression analysis revealed 249 genes that were significantly differentially expressed in AIH patients compared to controls. Using a random forest algorithm, we determined that less than 10 genes were sufficient to differentiate the two groups in our cohort. Interferon signaling was more active in AIH samples compared to controls, regardless of treatment status. Pegivirus sequences were detected in five AIH samples and 1 healthy sample. The gene expression data and clinical metadata were used to determine 12 genes that were significantly associated with advanced fibrosis in AIH. AIH patients with a partial response to therapy demonstrated decreased evidence of a CD8+ T cell gene expression signal. These findings represent progress in understanding a disease in need of better tests, therapies, and biomarkers.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0264307&type=printable |
| spellingShingle | Michele May-Sien Tana Arielle Klepper Amy Lyden Angela Oliveira Pisco Maira Phelps Breann McGee Kelsey Green Sandy Feng Joseph DeRisi Emily Dawn Crawford Craig S Lammert Transcriptomic profiling of blood from autoimmune hepatitis patients reveals potential mechanisms with implications for management. PLoS ONE |
| title | Transcriptomic profiling of blood from autoimmune hepatitis patients reveals potential mechanisms with implications for management. |
| title_full | Transcriptomic profiling of blood from autoimmune hepatitis patients reveals potential mechanisms with implications for management. |
| title_fullStr | Transcriptomic profiling of blood from autoimmune hepatitis patients reveals potential mechanisms with implications for management. |
| title_full_unstemmed | Transcriptomic profiling of blood from autoimmune hepatitis patients reveals potential mechanisms with implications for management. |
| title_short | Transcriptomic profiling of blood from autoimmune hepatitis patients reveals potential mechanisms with implications for management. |
| title_sort | transcriptomic profiling of blood from autoimmune hepatitis patients reveals potential mechanisms with implications for management |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0264307&type=printable |
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