DIVERSITY OF POTENTIAL BACTERIA IN PADDY FIELD WITH NATURAL AND CONVENTIONAL FARMING SYSTEMS
One of the causes of the rising use of chemical inputs is the high index of rice farming in Indonesia. It constitute a few of the factors contributing to Indonesia's declining rice fields' quality. This study aimed to classify and identify the diversity of potential bacteria in the rhizosp...
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Jurusan Agroteknologi, Fakultas Pertanian, Universitas Lampung
2025-01-01
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| Series: | Jurnal Agrotek Tropika |
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| Online Access: | https://jurnal.fp.unila.ac.id/index.php/JA/article/view/7767 |
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| author | Hadija Hadija Tutik Kuswinanti Muhammad Jayadi Sitti Halimah Larekeng |
| author_facet | Hadija Hadija Tutik Kuswinanti Muhammad Jayadi Sitti Halimah Larekeng |
| author_sort | Hadija Hadija |
| collection | DOAJ |
| description | One of the causes of the rising use of chemical inputs is the high index of rice farming in Indonesia. It constitute a few of the factors contributing to Indonesia's declining rice fields' quality. This study aimed to classify and identify the diversity of potential bacteria in the rhizosphere of rice plants. Methods this research used the enumeration method, biochemical test (bacteria characterization), and bacterial clustering. Further analysis of the clustering results is molecular identification to identify potential bacteria to the species level. The second location used a natural farming approach to calculate the amount of bacteria, the largest overall number of bacteria. The lowest number of isolates was obtained at a location with a conventional system. Thirty-two pure isolates were isolated from two rice fields in Sallasee Village, Bulukumba Regency. After clustering potential bacteria, six potential bacterial isolates were obtained in location two with natural farming systems. These bacteria are potential because they can fix nitrogen, dissolve phosphate, IAA, cellulosic enzymes, and catalase enzymes. Results Molecular species identification found three species of Bacillus Subtilis (LK.2.5, LK.2.12, and LK.2.1), also found two species of Brevundimonas with different species, namely (LK.2.13) isolate and Brevundimonas diminuta strain BZC3 isolate LK. 2.8. Meanwhile isolate LK.2.2 was identified as a Pseudomonas azotoformans strain S4. The similarity of identified bacterial species is between 97% - 99% with the Gen Bank data at NCBI. The functional ability of each bacterium varies greatly depending on the species and strain of each bacterium |
| format | Article |
| id | doaj-art-b3e53579bba340c4b8089e3767e178e3 |
| institution | Kabale University |
| issn | 2337-4993 2620-3138 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Jurusan Agroteknologi, Fakultas Pertanian, Universitas Lampung |
| record_format | Article |
| series | Jurnal Agrotek Tropika |
| spelling | doaj-art-b3e53579bba340c4b8089e3767e178e32025-08-20T03:45:43ZengJurusan Agroteknologi, Fakultas Pertanian, Universitas LampungJurnal Agrotek Tropika2337-49932620-31382025-01-0112476977610.23960/jat.v12i4.77675289DIVERSITY OF POTENTIAL BACTERIA IN PADDY FIELD WITH NATURAL AND CONVENTIONAL FARMING SYSTEMSHadija Hadija0Tutik Kuswinanti1Muhammad Jayadi2Sitti Halimah Larekeng3Program Studi Kehutatan, Universitas Muslim MarosDepartemen Ilmu Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Hasanuddin, Makassar.3Departemen Ilmu Tanah, Fakultas Pertanian, Universitas Hasanuddin Makassar, MakassarDepartemen Kehutanan, Fakultas Kehutanan, Universitas Hasanuddin Makassar, MakassarOne of the causes of the rising use of chemical inputs is the high index of rice farming in Indonesia. It constitute a few of the factors contributing to Indonesia's declining rice fields' quality. This study aimed to classify and identify the diversity of potential bacteria in the rhizosphere of rice plants. Methods this research used the enumeration method, biochemical test (bacteria characterization), and bacterial clustering. Further analysis of the clustering results is molecular identification to identify potential bacteria to the species level. The second location used a natural farming approach to calculate the amount of bacteria, the largest overall number of bacteria. The lowest number of isolates was obtained at a location with a conventional system. Thirty-two pure isolates were isolated from two rice fields in Sallasee Village, Bulukumba Regency. After clustering potential bacteria, six potential bacterial isolates were obtained in location two with natural farming systems. These bacteria are potential because they can fix nitrogen, dissolve phosphate, IAA, cellulosic enzymes, and catalase enzymes. Results Molecular species identification found three species of Bacillus Subtilis (LK.2.5, LK.2.12, and LK.2.1), also found two species of Brevundimonas with different species, namely (LK.2.13) isolate and Brevundimonas diminuta strain BZC3 isolate LK. 2.8. Meanwhile isolate LK.2.2 was identified as a Pseudomonas azotoformans strain S4. The similarity of identified bacterial species is between 97% - 99% with the Gen Bank data at NCBI. The functional ability of each bacterium varies greatly depending on the species and strain of each bacteriumhttps://jurnal.fp.unila.ac.id/index.php/JA/article/view/7767chemodiversity, clustering, functional bacteria, rice fields, soil quality |
| spellingShingle | Hadija Hadija Tutik Kuswinanti Muhammad Jayadi Sitti Halimah Larekeng DIVERSITY OF POTENTIAL BACTERIA IN PADDY FIELD WITH NATURAL AND CONVENTIONAL FARMING SYSTEMS Jurnal Agrotek Tropika chemodiversity, clustering, functional bacteria, rice fields, soil quality |
| title | DIVERSITY OF POTENTIAL BACTERIA IN PADDY FIELD WITH NATURAL AND CONVENTIONAL FARMING SYSTEMS |
| title_full | DIVERSITY OF POTENTIAL BACTERIA IN PADDY FIELD WITH NATURAL AND CONVENTIONAL FARMING SYSTEMS |
| title_fullStr | DIVERSITY OF POTENTIAL BACTERIA IN PADDY FIELD WITH NATURAL AND CONVENTIONAL FARMING SYSTEMS |
| title_full_unstemmed | DIVERSITY OF POTENTIAL BACTERIA IN PADDY FIELD WITH NATURAL AND CONVENTIONAL FARMING SYSTEMS |
| title_short | DIVERSITY OF POTENTIAL BACTERIA IN PADDY FIELD WITH NATURAL AND CONVENTIONAL FARMING SYSTEMS |
| title_sort | diversity of potential bacteria in paddy field with natural and conventional farming systems |
| topic | chemodiversity, clustering, functional bacteria, rice fields, soil quality |
| url | https://jurnal.fp.unila.ac.id/index.php/JA/article/view/7767 |
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