Field trial to correlate mineral solubilization activity of Pseudomonas aeruginosa and biochemical content of groundnut plants

The excessive use of phosphorus (P) fertilizers increases crop production but can lead to P-induced zinc (Zn) deficiencies, making both nutrients unavailable to plants. Plant–microbe interactions, such as with Pseudomonas aeruginosa, can alleviate these constraints by solubilizing Zn and P in soil....

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Main Authors: Krishnan Kutty Sunitha Kumari, Skandasamy Natchimuthu Padma Devi, Ranjithkumar Rajamani, Djearamane Sinouvassane, Tey Lai-Hock, Wong Ling Shing, Kayarohanam Saminathan, Arumugam Natarajan, Almansour Abdulrahman I., Mahalingam Sakkarapalayam M.
Format: Article
Language:English
Published: De Gruyter 2025-04-01
Series:Open Life Sciences
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Online Access:https://doi.org/10.1515/biol-2022-1008
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author Krishnan Kutty Sunitha Kumari
Skandasamy Natchimuthu Padma Devi
Ranjithkumar Rajamani
Djearamane Sinouvassane
Tey Lai-Hock
Wong Ling Shing
Kayarohanam Saminathan
Arumugam Natarajan
Almansour Abdulrahman I.
Mahalingam Sakkarapalayam M.
author_facet Krishnan Kutty Sunitha Kumari
Skandasamy Natchimuthu Padma Devi
Ranjithkumar Rajamani
Djearamane Sinouvassane
Tey Lai-Hock
Wong Ling Shing
Kayarohanam Saminathan
Arumugam Natarajan
Almansour Abdulrahman I.
Mahalingam Sakkarapalayam M.
author_sort Krishnan Kutty Sunitha Kumari
collection DOAJ
description The excessive use of phosphorus (P) fertilizers increases crop production but can lead to P-induced zinc (Zn) deficiencies, making both nutrients unavailable to plants. Plant–microbe interactions, such as with Pseudomonas aeruginosa, can alleviate these constraints by solubilizing Zn and P in soil. A soil incubation study revealed that applying P. aeruginosa with farmyard manure (FYM) significantly increased Zn and P solubilization (6.86 mg/l; 14.83 mg/l) compared to control (3.15 mg/l; 13.67 mg/l). A field experiment evaluated the effects of P. aeruginosa on the biochemical composition of groundnut plants under five treatments. The T2, T3, and T4 treatments had the highest protein, carbohydrate, and chlorophyll levels, likely due to the heterogeneous activity of FYM and the mineral solubilizing ability of P. aeruginosa. Groundnut seeds from T3 (combined liquid inoculant and FYM) had the highest iodine (88.47 mg KOH/g), saponification value (195.56 mg KOH/g), and free fatty acid content (2.23 g oleic acid). The pH of the T3 soil decreased from 8.3 to 7.5, and significant increases were observed in electrical conductivity (from 2.88 to 0.30 dS/m), calcium carbonate (2.53–1.7%), organic carbon (0.39–1.91%), nitrogen (273.75–788.25 kg/ha), P (20.1–59.65 kg/ha), potassium (182.25–346.5 kg/ha), and Zn (1.53–7.24 mg/kg). The study suggests that the combined application of liquid formulants of P. aeruginosa with FYM is advantageous, as FYM supports microbial growth by providing essential nutrients for mineralization. Moreover, liquid inoculants formulated with polyvinylpyrrolidone as an osmo protectant demonstrated enhanced shelf-life and mineral solubilization, contributing to improved biochemical properties in groundnut plants.
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spelling doaj-art-b3e4f5d8200142028dde1b38e89bbc732025-08-20T02:28:49ZengDe GruyterOpen Life Sciences2391-54122025-04-012014798010.1515/biol-2022-1008Field trial to correlate mineral solubilization activity of Pseudomonas aeruginosa and biochemical content of groundnut plantsKrishnan Kutty Sunitha Kumari0Skandasamy Natchimuthu Padma Devi1Ranjithkumar Rajamani2Djearamane Sinouvassane3Tey Lai-Hock4Wong Ling Shing5Kayarohanam Saminathan6Arumugam Natarajan7Almansour Abdulrahman I.8Mahalingam Sakkarapalayam M.9Department of Botany, PSGR Krishnammal College for Women, Peelamedu, Coimbatore, 641 004, Tamil Nadu, IndiaDepartment of Botany, PSGR Krishnammal College for Women, Peelamedu, Coimbatore, 641 004, Tamil Nadu, IndiaDepartment of Pharmacology, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 602105, Tamil Nadu, IndiaFaculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, Kampar, 31900, MalaysiaFaculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, Kampar, 31900, MalaysiaFaculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800Nilai, Negeri Sembilan, MalaysiaFaculty of Bioeconomics and Health sciences, Geomatika University Malaysia, Kuala Lumpur, 54200, MalaysiaDepartment of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh11451, Saudi ArabiaDepartment of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh11451, Saudi ArabiaDepartment of Chemistry, 720 Clinic Drive, Purdue University, West Lafayette, Indiana, 47907, United States of AmericaThe excessive use of phosphorus (P) fertilizers increases crop production but can lead to P-induced zinc (Zn) deficiencies, making both nutrients unavailable to plants. Plant–microbe interactions, such as with Pseudomonas aeruginosa, can alleviate these constraints by solubilizing Zn and P in soil. A soil incubation study revealed that applying P. aeruginosa with farmyard manure (FYM) significantly increased Zn and P solubilization (6.86 mg/l; 14.83 mg/l) compared to control (3.15 mg/l; 13.67 mg/l). A field experiment evaluated the effects of P. aeruginosa on the biochemical composition of groundnut plants under five treatments. The T2, T3, and T4 treatments had the highest protein, carbohydrate, and chlorophyll levels, likely due to the heterogeneous activity of FYM and the mineral solubilizing ability of P. aeruginosa. Groundnut seeds from T3 (combined liquid inoculant and FYM) had the highest iodine (88.47 mg KOH/g), saponification value (195.56 mg KOH/g), and free fatty acid content (2.23 g oleic acid). The pH of the T3 soil decreased from 8.3 to 7.5, and significant increases were observed in electrical conductivity (from 2.88 to 0.30 dS/m), calcium carbonate (2.53–1.7%), organic carbon (0.39–1.91%), nitrogen (273.75–788.25 kg/ha), P (20.1–59.65 kg/ha), potassium (182.25–346.5 kg/ha), and Zn (1.53–7.24 mg/kg). The study suggests that the combined application of liquid formulants of P. aeruginosa with FYM is advantageous, as FYM supports microbial growth by providing essential nutrients for mineralization. Moreover, liquid inoculants formulated with polyvinylpyrrolidone as an osmo protectant demonstrated enhanced shelf-life and mineral solubilization, contributing to improved biochemical properties in groundnut plants.https://doi.org/10.1515/biol-2022-1008pseudomonas aeruginosazincphosphorussolubilizationfield studycarbohydratesproteinschlorophylloilsoilfertility
spellingShingle Krishnan Kutty Sunitha Kumari
Skandasamy Natchimuthu Padma Devi
Ranjithkumar Rajamani
Djearamane Sinouvassane
Tey Lai-Hock
Wong Ling Shing
Kayarohanam Saminathan
Arumugam Natarajan
Almansour Abdulrahman I.
Mahalingam Sakkarapalayam M.
Field trial to correlate mineral solubilization activity of Pseudomonas aeruginosa and biochemical content of groundnut plants
Open Life Sciences
pseudomonas aeruginosa
zinc
phosphorus
solubilization
field study
carbohydrates
proteins
chlorophyll
oil
soil
fertility
title Field trial to correlate mineral solubilization activity of Pseudomonas aeruginosa and biochemical content of groundnut plants
title_full Field trial to correlate mineral solubilization activity of Pseudomonas aeruginosa and biochemical content of groundnut plants
title_fullStr Field trial to correlate mineral solubilization activity of Pseudomonas aeruginosa and biochemical content of groundnut plants
title_full_unstemmed Field trial to correlate mineral solubilization activity of Pseudomonas aeruginosa and biochemical content of groundnut plants
title_short Field trial to correlate mineral solubilization activity of Pseudomonas aeruginosa and biochemical content of groundnut plants
title_sort field trial to correlate mineral solubilization activity of pseudomonas aeruginosa and biochemical content of groundnut plants
topic pseudomonas aeruginosa
zinc
phosphorus
solubilization
field study
carbohydrates
proteins
chlorophyll
oil
soil
fertility
url https://doi.org/10.1515/biol-2022-1008
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