Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.
<h4>Aim</h4>To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice.<h4>Methods</h4>Ten animals were randomly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or p...
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Public Library of Science (PLoS)
2014-01-01
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| Online Access: | https://europepmc.org/articles/pmc3943729?pdf=render |
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| author | Ernesto Cesar Pinto Leal-Junior Patrícia de Almeida Shaiane Silva Tomazoni Paulo de Tarso Camillo de Carvalho Rodrigo Álvaro Brandão Lopes-Martins Lucio Frigo Jon Joensen Mark I Johnson Jan Magnus Bjordal |
| author_facet | Ernesto Cesar Pinto Leal-Junior Patrícia de Almeida Shaiane Silva Tomazoni Paulo de Tarso Camillo de Carvalho Rodrigo Álvaro Brandão Lopes-Martins Lucio Frigo Jon Joensen Mark I Johnson Jan Magnus Bjordal |
| author_sort | Ernesto Cesar Pinto Leal-Junior |
| collection | DOAJ |
| description | <h4>Aim</h4>To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice.<h4>Methods</h4>Ten animals were randomly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or placebo-LLLT at one point overlying the tibialis anterior muscle (bilaterally) 5 times per week for 14 weeks (from 6th to 20th week of age). Morphological changes, creatine kinase (CK) activity and mRNA gene expression were assessed in animals at 20th week of age.<h4>Results</h4>Animals treated with LLLT showed very few morphological changes in skeletal muscle, with less atrophy and fibrosis than animals treated with placebo-LLLT. CK was significantly lower (p=0.0203) in animals treated with LLLT (864.70 U.l-1, SEM 226.10) than placebo (1708.00 U.l-1, SEM 184.60). mRNA gene expression of inflammatory markers was significantly decreased by treatment with LLLT (p<0.05): TNF-α (placebo-control=0.51 µg/µl [SEM 0.12], - LLLT=0.048 µg/µl [SEM 0.01]), IL-1β (placebo-control=2.292 µg/µl [SEM 0.74], - LLLT=0.12 µg/µl [SEM 0.03]), IL-6 (placebo-control=3.946 µg/µl [SEM 0.98], - LLLT=0.854 µg/µl [SEM 0.33]), IL-10 (placebo-control=1.116 µg/µl [SEM 0.22], - LLLT=0.352 µg/µl [SEM 0.15]), and COX-2 (placebo-control=4.984 µg/µl [SEM 1.18], LLLT=1.470 µg/µl [SEM 0.73]).<h4>Conclusion</h4>Irradiation of superpulsed LLLT on successive days five times per week for 14 weeks decreased morphological changes, skeletal muscle damage and inflammation in mdx mice. This indicates that LLLT has potential to decrease progression of Duchenne muscular dystrophy. |
| format | Article |
| id | doaj-art-b3e1ea8339b643c4a9c76828962ee5cf |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
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| spelling | doaj-art-b3e1ea8339b643c4a9c76828962ee5cf2025-08-20T03:11:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e8945310.1371/journal.pone.0089453Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.Ernesto Cesar Pinto Leal-JuniorPatrícia de AlmeidaShaiane Silva TomazoniPaulo de Tarso Camillo de CarvalhoRodrigo Álvaro Brandão Lopes-MartinsLucio FrigoJon JoensenMark I JohnsonJan Magnus Bjordal<h4>Aim</h4>To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice.<h4>Methods</h4>Ten animals were randomly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or placebo-LLLT at one point overlying the tibialis anterior muscle (bilaterally) 5 times per week for 14 weeks (from 6th to 20th week of age). Morphological changes, creatine kinase (CK) activity and mRNA gene expression were assessed in animals at 20th week of age.<h4>Results</h4>Animals treated with LLLT showed very few morphological changes in skeletal muscle, with less atrophy and fibrosis than animals treated with placebo-LLLT. CK was significantly lower (p=0.0203) in animals treated with LLLT (864.70 U.l-1, SEM 226.10) than placebo (1708.00 U.l-1, SEM 184.60). mRNA gene expression of inflammatory markers was significantly decreased by treatment with LLLT (p<0.05): TNF-α (placebo-control=0.51 µg/µl [SEM 0.12], - LLLT=0.048 µg/µl [SEM 0.01]), IL-1β (placebo-control=2.292 µg/µl [SEM 0.74], - LLLT=0.12 µg/µl [SEM 0.03]), IL-6 (placebo-control=3.946 µg/µl [SEM 0.98], - LLLT=0.854 µg/µl [SEM 0.33]), IL-10 (placebo-control=1.116 µg/µl [SEM 0.22], - LLLT=0.352 µg/µl [SEM 0.15]), and COX-2 (placebo-control=4.984 µg/µl [SEM 1.18], LLLT=1.470 µg/µl [SEM 0.73]).<h4>Conclusion</h4>Irradiation of superpulsed LLLT on successive days five times per week for 14 weeks decreased morphological changes, skeletal muscle damage and inflammation in mdx mice. This indicates that LLLT has potential to decrease progression of Duchenne muscular dystrophy.https://europepmc.org/articles/pmc3943729?pdf=render |
| spellingShingle | Ernesto Cesar Pinto Leal-Junior Patrícia de Almeida Shaiane Silva Tomazoni Paulo de Tarso Camillo de Carvalho Rodrigo Álvaro Brandão Lopes-Martins Lucio Frigo Jon Joensen Mark I Johnson Jan Magnus Bjordal Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression. PLoS ONE |
| title | Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression. |
| title_full | Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression. |
| title_fullStr | Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression. |
| title_full_unstemmed | Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression. |
| title_short | Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression. |
| title_sort | superpulsed low level laser therapy protects skeletal muscle of mdx mice against damage inflammation and morphological changes delaying dystrophy progression |
| url | https://europepmc.org/articles/pmc3943729?pdf=render |
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