A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression
To evaluate the biological activity of Petiveria alliacea extracts on tumoral cells in vitro. Materials and methods. P.alliaceafractions prepared by a bioguided purification protocol were characterized by their biological activities on two human tumoral cell lines.Morphological changes, cell viabili...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pontificia Universidad Javeriana
2009-12-01
|
| Series: | Universitas Scientiarum |
| Subjects: | |
| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1382/845 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849691914605953024 |
|---|---|
| author | Susana Fiorentino Diana Mercedes Castañeda Claudia Patricia Urueña Maria Claudia Cifuentes |
| author_facet | Susana Fiorentino Diana Mercedes Castañeda Claudia Patricia Urueña Maria Claudia Cifuentes |
| author_sort | Susana Fiorentino |
| collection | DOAJ |
| description | To evaluate the biological activity of Petiveria alliacea extracts on tumoral cells in vitro. Materials and methods. P.alliaceafractions prepared by a bioguided purification protocol were characterized by their biological activities on two human tumoral cell lines.Morphological changes, cell viability, mitochondrial membrane depolarization, nuclear staining and activity on HSP70 were analyzed.Results. The present study demonstrates that P.alliacea fractions can induce apoptosis in a mitochondria-dependent pathway and downregulate HSP70 expression in vitro. The possible compounds present in P.alliacea responsible for the described biological activities wereidentified by dereplication methods. Conclusion. The present study provides new knowledge on the antitumoral properties of the plantspecies P. alliacea described in several ethnobotanical studies. |
| format | Article |
| id | doaj-art-b3dd65e9ab944cf0a36936007bb24ebe |
| institution | DOAJ |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2009-12-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-b3dd65e9ab944cf0a36936007bb24ebe2025-08-20T03:20:52ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522009-12-01142-3125134A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expressionSusana FiorentinoDiana Mercedes CastañedaClaudia Patricia UrueñaMaria Claudia CifuentesTo evaluate the biological activity of Petiveria alliacea extracts on tumoral cells in vitro. Materials and methods. P.alliaceafractions prepared by a bioguided purification protocol were characterized by their biological activities on two human tumoral cell lines.Morphological changes, cell viability, mitochondrial membrane depolarization, nuclear staining and activity on HSP70 were analyzed.Results. The present study demonstrates that P.alliacea fractions can induce apoptosis in a mitochondria-dependent pathway and downregulate HSP70 expression in vitro. The possible compounds present in P.alliacea responsible for the described biological activities wereidentified by dereplication methods. Conclusion. The present study provides new knowledge on the antitumoral properties of the plantspecies P. alliacea described in several ethnobotanical studies.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1382/845Apoptosiscancerheat shock proteinsPetiveria alliacea |
| spellingShingle | Susana Fiorentino Diana Mercedes Castañeda Claudia Patricia Urueña Maria Claudia Cifuentes A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression Universitas Scientiarum Apoptosis cancer heat shock proteins Petiveria alliacea |
| title | A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression |
| title_full | A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression |
| title_fullStr | A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression |
| title_full_unstemmed | A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression |
| title_short | A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression |
| title_sort | fraction from petiveria alliacea induces apoptosis via a mitochondria dependent pathway and regulates hsp70 expression |
| topic | Apoptosis cancer heat shock proteins Petiveria alliacea |
| url | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1382/845 |
| work_keys_str_mv | AT susanafiorentino afractionfrompetiveriaalliaceainducesapoptosisviaamitochondriadependentpathwayandregulateshsp70expression AT dianamercedescastaneda afractionfrompetiveriaalliaceainducesapoptosisviaamitochondriadependentpathwayandregulateshsp70expression AT claudiapatriciauruena afractionfrompetiveriaalliaceainducesapoptosisviaamitochondriadependentpathwayandregulateshsp70expression AT mariaclaudiacifuentes afractionfrompetiveriaalliaceainducesapoptosisviaamitochondriadependentpathwayandregulateshsp70expression AT susanafiorentino fractionfrompetiveriaalliaceainducesapoptosisviaamitochondriadependentpathwayandregulateshsp70expression AT dianamercedescastaneda fractionfrompetiveriaalliaceainducesapoptosisviaamitochondriadependentpathwayandregulateshsp70expression AT claudiapatriciauruena fractionfrompetiveriaalliaceainducesapoptosisviaamitochondriadependentpathwayandregulateshsp70expression AT mariaclaudiacifuentes fractionfrompetiveriaalliaceainducesapoptosisviaamitochondriadependentpathwayandregulateshsp70expression |