Characterization and molecular biological identification of tick species in Nanjing city
ObjectiveTo investigate and analyze the habitat and host distribution characteristics of tick species in Nanjing city to provide a basis for the prevention and control of ticks and tick-borne diseases. MethodsFrom April to September 2023, free-living and parasitic ticks were collected in Nanjing cit...
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Editorial Office of Chinese Journal of Public Health
2024-12-01
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| Series: | Zhongguo gonggong weisheng |
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| Online Access: | https://www.zgggws.com/article/doi/10.11847/zgggws1145423 |
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| author | Luyao SUN Xuemin CHEN Bingdong SONG Yan ZHANG Yanqun SUN Yue MIN Shougang ZHANG |
| author_facet | Luyao SUN Xuemin CHEN Bingdong SONG Yan ZHANG Yanqun SUN Yue MIN Shougang ZHANG |
| author_sort | Luyao SUN |
| collection | DOAJ |
| description | ObjectiveTo investigate and analyze the habitat and host distribution characteristics of tick species in Nanjing city to provide a basis for the prevention and control of ticks and tick-borne diseases. MethodsFrom April to September 2023, free-living and parasitic ticks were collected in Nanjing city by drag-flag method and body surface examination, respectively. The ticks were identified by morphological classification and molecular biological identification based on 16S rRNA gene. ResultsA total of 1 523 ticks were collected, including 993 Haemaphysalis longicornis (65.20% of all ticks collected), 529 Haemaphysalis flava (34.73%), and one Haemaphysalis phasiana (0.07%). The highest number of free-living ticks in different habitats was collected in suburban parks (50), followed by those around rural natural villages (47) and barren grasslands (33), and then in forest areas (2). Parasitic ticks were collected from goats, dogs, and hedgehogs, which accounted for 55.79% (776 ticks), 9.35% (130), and 34.87% (485) of the 1 391 parasitic ticks collected, respectively. Homology analysis showed that individuals of the same tick species originated from the same evolutionary branch. ConclusionsThree tick species, H. longicornis, H. flava, and H. phasiana, were identified in Nanjing city. Haemaphysalis longicornis was the dominant species, and H. phasiana was identified as a newly monitored tick species in the region. Tick species composition varied among habitats and animal hosts. No significant genetic variation within tick species was observed. |
| format | Article |
| id | doaj-art-b3c001a1e22e4e6a904136062f66a0fa |
| institution | Kabale University |
| issn | 1001-0580 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Editorial Office of Chinese Journal of Public Health |
| record_format | Article |
| series | Zhongguo gonggong weisheng |
| spelling | doaj-art-b3c001a1e22e4e6a904136062f66a0fa2025-02-11T02:11:59ZzhoEditorial Office of Chinese Journal of Public HealthZhongguo gonggong weisheng1001-05802024-12-0140121505151010.11847/zgggws11454231145423Characterization and molecular biological identification of tick species in Nanjing cityLuyao SUN0Xuemin CHEN1Bingdong SONG2Yan ZHANG3Yanqun SUN4Yue MIN5Shougang ZHANG6Nanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, ChinaNanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, ChinaNanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, ChinaNanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, ChinaNanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, ChinaNanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, ChinaNanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, ChinaObjectiveTo investigate and analyze the habitat and host distribution characteristics of tick species in Nanjing city to provide a basis for the prevention and control of ticks and tick-borne diseases. MethodsFrom April to September 2023, free-living and parasitic ticks were collected in Nanjing city by drag-flag method and body surface examination, respectively. The ticks were identified by morphological classification and molecular biological identification based on 16S rRNA gene. ResultsA total of 1 523 ticks were collected, including 993 Haemaphysalis longicornis (65.20% of all ticks collected), 529 Haemaphysalis flava (34.73%), and one Haemaphysalis phasiana (0.07%). The highest number of free-living ticks in different habitats was collected in suburban parks (50), followed by those around rural natural villages (47) and barren grasslands (33), and then in forest areas (2). Parasitic ticks were collected from goats, dogs, and hedgehogs, which accounted for 55.79% (776 ticks), 9.35% (130), and 34.87% (485) of the 1 391 parasitic ticks collected, respectively. Homology analysis showed that individuals of the same tick species originated from the same evolutionary branch. ConclusionsThree tick species, H. longicornis, H. flava, and H. phasiana, were identified in Nanjing city. Haemaphysalis longicornis was the dominant species, and H. phasiana was identified as a newly monitored tick species in the region. Tick species composition varied among habitats and animal hosts. No significant genetic variation within tick species was observed.https://www.zgggws.com/article/doi/10.11847/zgggws1145423tickpopulation distribution16s rrna genephylogenetic analysisnanjing city |
| spellingShingle | Luyao SUN Xuemin CHEN Bingdong SONG Yan ZHANG Yanqun SUN Yue MIN Shougang ZHANG Characterization and molecular biological identification of tick species in Nanjing city Zhongguo gonggong weisheng tick population distribution 16s rrna gene phylogenetic analysis nanjing city |
| title | Characterization and molecular biological identification of tick species in Nanjing city |
| title_full | Characterization and molecular biological identification of tick species in Nanjing city |
| title_fullStr | Characterization and molecular biological identification of tick species in Nanjing city |
| title_full_unstemmed | Characterization and molecular biological identification of tick species in Nanjing city |
| title_short | Characterization and molecular biological identification of tick species in Nanjing city |
| title_sort | characterization and molecular biological identification of tick species in nanjing city |
| topic | tick population distribution 16s rrna gene phylogenetic analysis nanjing city |
| url | https://www.zgggws.com/article/doi/10.11847/zgggws1145423 |
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