Protective effects of turmeric extract, curcumin, demethoxycurcumin, bis-demethoxycurcumin, and ar-turmerone from Curcuma longa L. rhizomes on acetaminophen-induced hepatotoxicity in HepG2 cells

Protective effects of turmeric extract, curcumin, demethoxycurcumin, bis-demethoxycurcumin, and ar-turmerone from Curcuma longa L. rhizomes on acetaminophen-induced hepatotoxicity in HepG2 cells

Objective: To assess the effects of turmeric extract and its compounds on oxidative stress, inflammation, and apoptosis in acetaminophen-induced liver injury. Methods: HepG2 cells were administered with acetaminophen (40 mM) to induce hepatotoxicity, followed by treatment with turmeric extract and i...

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Main Authors: Wahyu Widowati, Dian Ratih Laksmitawati, Diah Kartika Pratami, Deni Rahmat, S. Ravi Kiran, J. Achyutha Devi, Didik Priyandoko, Nindia Salsabila Mia Dewi, Annisa Firdaus Sutendi, Rizal Azis, Dhanar Septyawan Hadiprasetyo, Mariska Elizabeth
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-06-01
Series:Asian Pacific Journal of Tropical Biomedicine
Subjects:
acetaminophen
apoptosis
curcuma longa
curcumin
hepatotoxicity
inflammation
oxidative stress
Online Access:https://journals.lww.com/10.4103/apjtb.apjtb_770_24
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Summary:Objective: To assess the effects of turmeric extract and its compounds on oxidative stress, inflammation, and apoptosis in acetaminophen-induced liver injury. Methods: HepG2 cells were administered with acetaminophen (40 mM) to induce hepatotoxicity, followed by treatment with turmeric extract and its isolated compounds including curcumin, demethoxycurcumin, bis-demethoxycurcumin and ar-turmerone at 5, 25, and 125 μg/mL. IL-1β, IL-6, and IL-10 levels were quantified with ELISA kits. Further, qRT-PCR was used to analyze the mRNA expression of JNK, Casp-9, and Casp-3. Meanwhile, the levels of nitric oxide and lactate dehydrogenase were analyzed using colorimetric assay. Results: Acetaminophen administration caused an increase in the levels of lactate dehydrogenase, nitric oxide, IL-1β, IL-6, and the mRNA expression of JNK, Casp-9, and Casp-3 in HepG2 cells while reducing IL-10 levels. Treatment with turmeric extract, curcumin, demethoxycurcumin, bis-demethoxycurcumin, and ar-turmerone lowered IL-1β, IL-6, nitric oxide, and lactate dehydrogenase levels, downregulated the mRNA expression of JNK, Casp-9, and Casp-3, and increased IL-10 levels. Conclusions: Turmeric extract and its compounds have significant hepatoprotective activity and could be further explored for the treatment of liver damage.
ISSN:2221-1691
2588-9222

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