EFFECT OF RED CABBAGE CRUDE EXTRACTS ON E. coli AND Staph.aureus GROWTH

The current study concentrated on effect of red cabbage crude extracts, first: acidified methanol extract by 2.4 mM/L HCl, second: of methanol 80 %, on growth of Escherichia coli and Staphylococcus aureus in solid and liquid media. Each extract was added in 125, 250, and 500 µL/ treatment , it was a...

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Main Authors: Salah Ahmed, Muhannad Al-Taleb
Format: Article
Language:English
Published: College of Agriculture 2018-09-01
Series:Mesopotamia Journal of Agriculture
Online Access:https://magrj.mosuljournals.com/article_161503_d41d8cd98f00b204e9800998ecf8427e.pdf
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author Salah Ahmed
Muhannad Al-Taleb
author_facet Salah Ahmed
Muhannad Al-Taleb
author_sort Salah Ahmed
collection DOAJ
description The current study concentrated on effect of red cabbage crude extracts, first: acidified methanol extract by 2.4 mM/L HCl, second: of methanol 80 %, on growth of Escherichia coli and Staphylococcus aureus in solid and liquid media. Each extract was added in 125, 250, and 500 µL/ treatment , it was added to the fixed sterilized disks in solid medium or added to liquid medium, however, the control treatment freed of extracts. All treatments were incubated for 24, 48, and 72 hours at 30 oC when inhibition percentages were calculated and the minimum inhibition concentration (MIC) was measured for both bacteria. The results appeared that red cabbage crude extracts showed significant (p<0.05) change on E. coli and Staph. aureus inhibition in the solid media as the inhibition ratios on ranged between 42.59-92.17 and 40.04-86.60% , respectively , while the inhibition ratios in the liquid media between 46.44-96.26  and 44.45-95.10% for the two species of bacteria respectively. The lowest  MIC  occurred when adding acidified methanol by HCl of red cabbage especially against E. coli as inhibition happened at 200 µL and this lower than the concentration which inhibited Staph.aureus.
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spelling doaj-art-b3a5ce7c5be149f0a84eaaaa40889e142025-08-20T02:23:14ZengCollege of AgricultureMesopotamia Journal of Agriculture1815-316X2224-97962018-09-0146322923810.33899/magrj.2018.161503161503EFFECT OF RED CABBAGE CRUDE EXTRACTS ON E. coli AND Staph.aureus GROWTHSalah Ahmed0Muhannad Al-Taleb1Food Sci.Dept., College of Agriculture and Forestry, Mosul University. IraqFood Sci.Dept. / College of Agric.& Forest./ Mosul Univ. / IraqThe current study concentrated on effect of red cabbage crude extracts, first: acidified methanol extract by 2.4 mM/L HCl, second: of methanol 80 %, on growth of Escherichia coli and Staphylococcus aureus in solid and liquid media. Each extract was added in 125, 250, and 500 µL/ treatment , it was added to the fixed sterilized disks in solid medium or added to liquid medium, however, the control treatment freed of extracts. All treatments were incubated for 24, 48, and 72 hours at 30 oC when inhibition percentages were calculated and the minimum inhibition concentration (MIC) was measured for both bacteria. The results appeared that red cabbage crude extracts showed significant (p<0.05) change on E. coli and Staph. aureus inhibition in the solid media as the inhibition ratios on ranged between 42.59-92.17 and 40.04-86.60% , respectively , while the inhibition ratios in the liquid media between 46.44-96.26  and 44.45-95.10% for the two species of bacteria respectively. The lowest  MIC  occurred when adding acidified methanol by HCl of red cabbage especially against E. coli as inhibition happened at 200 µL and this lower than the concentration which inhibited Staph.aureus.https://magrj.mosuljournals.com/article_161503_d41d8cd98f00b204e9800998ecf8427e.pdf
spellingShingle Salah Ahmed
Muhannad Al-Taleb
EFFECT OF RED CABBAGE CRUDE EXTRACTS ON E. coli AND Staph.aureus GROWTH
Mesopotamia Journal of Agriculture
title EFFECT OF RED CABBAGE CRUDE EXTRACTS ON E. coli AND Staph.aureus GROWTH
title_full EFFECT OF RED CABBAGE CRUDE EXTRACTS ON E. coli AND Staph.aureus GROWTH
title_fullStr EFFECT OF RED CABBAGE CRUDE EXTRACTS ON E. coli AND Staph.aureus GROWTH
title_full_unstemmed EFFECT OF RED CABBAGE CRUDE EXTRACTS ON E. coli AND Staph.aureus GROWTH
title_short EFFECT OF RED CABBAGE CRUDE EXTRACTS ON E. coli AND Staph.aureus GROWTH
title_sort effect of red cabbage crude extracts on e coli and staph aureus growth
url https://magrj.mosuljournals.com/article_161503_d41d8cd98f00b204e9800998ecf8427e.pdf
work_keys_str_mv AT salahahmed effectofredcabbagecrudeextractsonecoliandstaphaureusgrowth
AT muhannadaltaleb effectofredcabbagecrudeextractsonecoliandstaphaureusgrowth