Bone substitute made from a Brazilian oyster shell functions as a fast stimulator for bone-forming cells in an animal model.
Despite their demonstrated biocompatibility and osteogenic properties, oyster shells have been reported as a potential alternative to other commonly used materials for bone substitution. This study evaluated whether an experimental bone substitute (EBS) made from a typical oyster shell of Northeaste...
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0198697&type=printable |
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| author | Ricardo Coringa Eduardo Martins de Sousa Juliana Nunes Botelho Rafael Soares Diniz Joicy Cortez de Sá Maria Carmen Fontoura Nogueira da Cruz Marco Aurelio Beninni Paschoal Letícia Machado Gonçalves |
| author_facet | Ricardo Coringa Eduardo Martins de Sousa Juliana Nunes Botelho Rafael Soares Diniz Joicy Cortez de Sá Maria Carmen Fontoura Nogueira da Cruz Marco Aurelio Beninni Paschoal Letícia Machado Gonçalves |
| author_sort | Ricardo Coringa |
| collection | DOAJ |
| description | Despite their demonstrated biocompatibility and osteogenic properties, oyster shells have been reported as a potential alternative to other commonly used materials for bone substitution. This study evaluated whether an experimental bone substitute (EBS) made from a typical oyster shell of Northeastern Brazil (Crassostrea rhizophora) has effects on bone development using an animal model. Oysters were collected from a biologically assisted vivarium, and their inner layer was used for preparing an EBS. Chemical and surface characterization of EBS was performed using Individually Coupled Plasma Optical Emission Spectrometry (ICP-OES) and Scanning Electron Microscope (SEM), respectively. Seventy-two rats were randomly assigned to groups according to the treatment of bone defects created in the submandibular area: Negative Control (-C), Positive Control (+C; Bio-Oss®) and EBS. Euthanasia occurred at 7, 21, 42 and 56 days postoperatively. The bone pieces were stained with hematoxylin and eosin (H&E). The formation of bone tissue was evaluated histologically and histomorphometrically. Data were analyzed through the Kruskal-Wallis test and ANOVA considering a significant level of 5%. The main element found in EBS was calcium (71.68%), and it presented heterogeneity in the particle size and a porosity aspect at SEM analysis. Histological results revealed the absence of inflammatory cells in all groups, being that EBS presented the most accelerated process of bone formation with a statistically significant difference between this group and the +C and -C groups in the 21-day time-point (p < 0.05). After 21 days, the bone formation process was similar between all groups (p > 0.05), showing an immature lamellar bone pattern after 56 days of experimentation (p > 0.05). Within the limitations of this study, it was possible to conclude that EBS presented good biocompatibility and promoted fast stimulation for bone-forming cells in an animal model. |
| format | Article |
| id | doaj-art-b399ac8e0bcb45ca80939e5ad06bddc5 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-b399ac8e0bcb45ca80939e5ad06bddc52025-08-20T02:03:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019869710.1371/journal.pone.0198697Bone substitute made from a Brazilian oyster shell functions as a fast stimulator for bone-forming cells in an animal model.Ricardo CoringaEduardo Martins de SousaJuliana Nunes BotelhoRafael Soares DinizJoicy Cortez de SáMaria Carmen Fontoura Nogueira da CruzMarco Aurelio Beninni PaschoalLetícia Machado GonçalvesDespite their demonstrated biocompatibility and osteogenic properties, oyster shells have been reported as a potential alternative to other commonly used materials for bone substitution. This study evaluated whether an experimental bone substitute (EBS) made from a typical oyster shell of Northeastern Brazil (Crassostrea rhizophora) has effects on bone development using an animal model. Oysters were collected from a biologically assisted vivarium, and their inner layer was used for preparing an EBS. Chemical and surface characterization of EBS was performed using Individually Coupled Plasma Optical Emission Spectrometry (ICP-OES) and Scanning Electron Microscope (SEM), respectively. Seventy-two rats were randomly assigned to groups according to the treatment of bone defects created in the submandibular area: Negative Control (-C), Positive Control (+C; Bio-Oss®) and EBS. Euthanasia occurred at 7, 21, 42 and 56 days postoperatively. The bone pieces were stained with hematoxylin and eosin (H&E). The formation of bone tissue was evaluated histologically and histomorphometrically. Data were analyzed through the Kruskal-Wallis test and ANOVA considering a significant level of 5%. The main element found in EBS was calcium (71.68%), and it presented heterogeneity in the particle size and a porosity aspect at SEM analysis. Histological results revealed the absence of inflammatory cells in all groups, being that EBS presented the most accelerated process of bone formation with a statistically significant difference between this group and the +C and -C groups in the 21-day time-point (p < 0.05). After 21 days, the bone formation process was similar between all groups (p > 0.05), showing an immature lamellar bone pattern after 56 days of experimentation (p > 0.05). Within the limitations of this study, it was possible to conclude that EBS presented good biocompatibility and promoted fast stimulation for bone-forming cells in an animal model.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0198697&type=printable |
| spellingShingle | Ricardo Coringa Eduardo Martins de Sousa Juliana Nunes Botelho Rafael Soares Diniz Joicy Cortez de Sá Maria Carmen Fontoura Nogueira da Cruz Marco Aurelio Beninni Paschoal Letícia Machado Gonçalves Bone substitute made from a Brazilian oyster shell functions as a fast stimulator for bone-forming cells in an animal model. PLoS ONE |
| title | Bone substitute made from a Brazilian oyster shell functions as a fast stimulator for bone-forming cells in an animal model. |
| title_full | Bone substitute made from a Brazilian oyster shell functions as a fast stimulator for bone-forming cells in an animal model. |
| title_fullStr | Bone substitute made from a Brazilian oyster shell functions as a fast stimulator for bone-forming cells in an animal model. |
| title_full_unstemmed | Bone substitute made from a Brazilian oyster shell functions as a fast stimulator for bone-forming cells in an animal model. |
| title_short | Bone substitute made from a Brazilian oyster shell functions as a fast stimulator for bone-forming cells in an animal model. |
| title_sort | bone substitute made from a brazilian oyster shell functions as a fast stimulator for bone forming cells in an animal model |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0198697&type=printable |
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