Study on Detection Method of Sulfamethazine Residues in Duck Blood Based on Surface-Enhanced Raman Spectroscopy
Sulfadimethazine (SM2) is widely used in livestock and poultry farming, but its improper use can pose a serious threat to human health. Therefore, the detection of SM2 residues in livestock and poultry products, including duck blood, is of great significance for food safety. A rapid detection method...
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MDPI AG
2025-05-01
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| author | Junshi Huang Runhua Zhou Jinlong Lin Qi Chen Ping Liu Shuanggen Huang Jinhui Zhao |
| author_facet | Junshi Huang Runhua Zhou Jinlong Lin Qi Chen Ping Liu Shuanggen Huang Jinhui Zhao |
| author_sort | Junshi Huang |
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| description | Sulfadimethazine (SM2) is widely used in livestock and poultry farming, but its improper use can pose a serious threat to human health. Therefore, the detection of SM2 residues in livestock and poultry products, including duck blood, is of great significance for food safety. A rapid detection method for SM2 residues in duck blood based on surface-enhanced Raman spectroscopy (SERS) was proposed in this paper. Density functional theory (DFT) was employed to optimize the molecular structure of SM2 and perform theoretical Raman vibrational analysis, thereby identifying its characteristic peaks. The enhancement effects of four different substrates were compared. The sample pretreatment method and detection conditions were optimized through single-factor experiments, including the types and amounts of electrolyte aggregators, the amount of gold nanocolloids, and the adsorption time. Under optimal conditions, the SERS spectral data of the samples were preprocessed, and features were extracted to establish an optimal quantitative prediction model. The experimental results found that the adaptive iteratively reweighted penalized least-squares method (air-PLS) was the best preprocessing method, and the competitive adaptive reweighted sampling–multiple linear regression (CARS-MLR) model demonstrated the best prediction performance, with a coefficient of determination for the prediction set (R<sub>p</sub><sup>2</sup>) of 0.9817, a root mean square error of calibration (RMSEC) of 1.5539 mg/L, a relative prediction deviation (RPD) of 7.1953, and limits of quantification of 0.75 mg/L. The research demonstrated that the combination of SERS technology and chemometric methods was feasible and effective for the detection of SM2 residues in duck blood. |
| format | Article |
| id | doaj-art-b3991f45d29947d2b81967d0c3edbda4 |
| institution | DOAJ |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Biosensors |
| spelling | doaj-art-b3991f45d29947d2b81967d0c3edbda42025-08-20T03:14:32ZengMDPI AGBiosensors2079-63742025-05-0115528610.3390/bios15050286Study on Detection Method of Sulfamethazine Residues in Duck Blood Based on Surface-Enhanced Raman SpectroscopyJunshi Huang0Runhua Zhou1Jinlong Lin2Qi Chen3Ping Liu4Shuanggen Huang5Jinhui Zhao6Key Laboratory of Modern Agricultural Equipment of Jiangxi, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Modern Agricultural Equipment of Jiangxi, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Modern Agricultural Equipment of Jiangxi, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Modern Agricultural Equipment of Jiangxi, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Modern Agricultural Equipment of Jiangxi, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Modern Agricultural Equipment of Jiangxi, Jiangxi Agricultural University, Nanchang 330045, ChinaSulfadimethazine (SM2) is widely used in livestock and poultry farming, but its improper use can pose a serious threat to human health. Therefore, the detection of SM2 residues in livestock and poultry products, including duck blood, is of great significance for food safety. A rapid detection method for SM2 residues in duck blood based on surface-enhanced Raman spectroscopy (SERS) was proposed in this paper. Density functional theory (DFT) was employed to optimize the molecular structure of SM2 and perform theoretical Raman vibrational analysis, thereby identifying its characteristic peaks. The enhancement effects of four different substrates were compared. The sample pretreatment method and detection conditions were optimized through single-factor experiments, including the types and amounts of electrolyte aggregators, the amount of gold nanocolloids, and the adsorption time. Under optimal conditions, the SERS spectral data of the samples were preprocessed, and features were extracted to establish an optimal quantitative prediction model. The experimental results found that the adaptive iteratively reweighted penalized least-squares method (air-PLS) was the best preprocessing method, and the competitive adaptive reweighted sampling–multiple linear regression (CARS-MLR) model demonstrated the best prediction performance, with a coefficient of determination for the prediction set (R<sub>p</sub><sup>2</sup>) of 0.9817, a root mean square error of calibration (RMSEC) of 1.5539 mg/L, a relative prediction deviation (RPD) of 7.1953, and limits of quantification of 0.75 mg/L. The research demonstrated that the combination of SERS technology and chemometric methods was feasible and effective for the detection of SM2 residues in duck blood.https://www.mdpi.com/2079-6374/15/5/286duck bloodsurface-enhanced Raman spectroscopysulfamethazinedensity functional theory |
| spellingShingle | Junshi Huang Runhua Zhou Jinlong Lin Qi Chen Ping Liu Shuanggen Huang Jinhui Zhao Study on Detection Method of Sulfamethazine Residues in Duck Blood Based on Surface-Enhanced Raman Spectroscopy Biosensors duck blood surface-enhanced Raman spectroscopy sulfamethazine density functional theory |
| title | Study on Detection Method of Sulfamethazine Residues in Duck Blood Based on Surface-Enhanced Raman Spectroscopy |
| title_full | Study on Detection Method of Sulfamethazine Residues in Duck Blood Based on Surface-Enhanced Raman Spectroscopy |
| title_fullStr | Study on Detection Method of Sulfamethazine Residues in Duck Blood Based on Surface-Enhanced Raman Spectroscopy |
| title_full_unstemmed | Study on Detection Method of Sulfamethazine Residues in Duck Blood Based on Surface-Enhanced Raman Spectroscopy |
| title_short | Study on Detection Method of Sulfamethazine Residues in Duck Blood Based on Surface-Enhanced Raman Spectroscopy |
| title_sort | study on detection method of sulfamethazine residues in duck blood based on surface enhanced raman spectroscopy |
| topic | duck blood surface-enhanced Raman spectroscopy sulfamethazine density functional theory |
| url | https://www.mdpi.com/2079-6374/15/5/286 |
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