Peptidomic analysis of whey protein hydrolysates and prediction of their antioxidant peptides
Enzymatic hydrolysis of proteins is a breakdown process of peptide bond in proteins, releasing some peptides with potential biological functions. Previous studies on enzymatic hydrolysis of whey proteins have not identified the complete peptide profiles after hydrolysis. In this study, we reconstruc...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021001075 |
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| author | Jesus Morales García Chibuike C. Udenigwe Jorge Duitama Andrés Fernando González Barrios |
| author_facet | Jesus Morales García Chibuike C. Udenigwe Jorge Duitama Andrés Fernando González Barrios |
| author_sort | Jesus Morales García |
| collection | DOAJ |
| description | Enzymatic hydrolysis of proteins is a breakdown process of peptide bond in proteins, releasing some peptides with potential biological functions. Previous studies on enzymatic hydrolysis of whey proteins have not identified the complete peptide profiles after hydrolysis. In this study, we reconstructed a profile of peptides from whey hydrolysates with two enzymes and different processing conditions. We also developed an ensemble machine learning predictor to classify peptides obtained from whey hydrolysis. A total of 2572 peptides were identified over three process conditions with two enzymes in duplicate. 499 peptides were classified and chosen as potential antioxidant peptides from whey proteins. The peptides classified as antioxidants in the hydrolysates had a proportion of 13.1%–24.5% regarding all peptides identified. These results facilitate the selection of promising peptides involved in the antioxidant properties during the enzymatic hydrolysis of whey proteins, aiding the discovery of novel antioxidant peptides. |
| format | Article |
| id | doaj-art-b3956f2a63104f02a9ebf0e5e05fc517 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-b3956f2a63104f02a9ebf0e5e05fc5172025-02-03T06:49:00ZengTsinghua University PressFood Science and Human Wellness2213-45302022-03-01112349355Peptidomic analysis of whey protein hydrolysates and prediction of their antioxidant peptidesJesus Morales García0Chibuike C. Udenigwe1Jorge Duitama2Andrés Fernando González Barrios3Grupo de Diseño de Productos y Procesos (GDPP), Department of Chemical and Food Engineering, Universidad de los Andes, Bogotá 111711, ColombiaSchool of Nutrition Sciences, University of Ottawa, Ottawa, Ontario, K1N 6N5, CanadaSystems and Computing Engineering Department, Universidad de Los Andes, Bogotá 111711, ColombiaGrupo de Diseño de Productos y Procesos (GDPP), Department of Chemical and Food Engineering, Universidad de los Andes, Bogotá 111711, Colombia; Corresponding author.Enzymatic hydrolysis of proteins is a breakdown process of peptide bond in proteins, releasing some peptides with potential biological functions. Previous studies on enzymatic hydrolysis of whey proteins have not identified the complete peptide profiles after hydrolysis. In this study, we reconstructed a profile of peptides from whey hydrolysates with two enzymes and different processing conditions. We also developed an ensemble machine learning predictor to classify peptides obtained from whey hydrolysis. A total of 2572 peptides were identified over three process conditions with two enzymes in duplicate. 499 peptides were classified and chosen as potential antioxidant peptides from whey proteins. The peptides classified as antioxidants in the hydrolysates had a proportion of 13.1%–24.5% regarding all peptides identified. These results facilitate the selection of promising peptides involved in the antioxidant properties during the enzymatic hydrolysis of whey proteins, aiding the discovery of novel antioxidant peptides.http://www.sciencedirect.com/science/article/pii/S2213453021001075Whey hydrolysateAntioxidant peptidePeptidomicsPrediction |
| spellingShingle | Jesus Morales García Chibuike C. Udenigwe Jorge Duitama Andrés Fernando González Barrios Peptidomic analysis of whey protein hydrolysates and prediction of their antioxidant peptides Food Science and Human Wellness Whey hydrolysate Antioxidant peptide Peptidomics Prediction |
| title | Peptidomic analysis of whey protein hydrolysates and prediction of their antioxidant peptides |
| title_full | Peptidomic analysis of whey protein hydrolysates and prediction of their antioxidant peptides |
| title_fullStr | Peptidomic analysis of whey protein hydrolysates and prediction of their antioxidant peptides |
| title_full_unstemmed | Peptidomic analysis of whey protein hydrolysates and prediction of their antioxidant peptides |
| title_short | Peptidomic analysis of whey protein hydrolysates and prediction of their antioxidant peptides |
| title_sort | peptidomic analysis of whey protein hydrolysates and prediction of their antioxidant peptides |
| topic | Whey hydrolysate Antioxidant peptide Peptidomics Prediction |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021001075 |
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