Dynamic meiotic behavior and evolutionary insights of supernumerary B chromosomes in the hangingfly Bittacus cirratus (Mecoptera, Bittacidae)

Supernumerary B chromosomes are significant dispensable genetic elements that follow their own species-specific evolutionary pathways. Despite their widespread occurrence, comprehensive analyses of their meiotic behavior remain limited. In this study, we present the first systematic investigation of...

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Main Authors: Le-Le He, Ying Miao, He-Hong Wang, Jie Zhang, Bao-Zhen Hua
Format: Article
Language:English
Published: Pensoft Publishers 2025-06-01
Series:Comparative Cytogenetics
Online Access:https://compcytogen.pensoft.net/article/153340/download/pdf/
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author Le-Le He
Ying Miao
He-Hong Wang
Jie Zhang
Bao-Zhen Hua
author_facet Le-Le He
Ying Miao
He-Hong Wang
Jie Zhang
Bao-Zhen Hua
author_sort Le-Le He
collection DOAJ
description Supernumerary B chromosomes are significant dispensable genetic elements that follow their own species-specific evolutionary pathways. Despite their widespread occurrence, comprehensive analyses of their meiotic behavior remain limited. In this study, we present the first systematic investigation of B chromosome morphology and meiotic behavior in the hangingfly Bittacus cirratus Tjeder, 1956 using cytogenetic approaches. The male basal chromosome numbers of B. cirratus is 2n = 30 + XO, with 0–5 polymorphic B chromosomes. Intraspecific B chromosome polymorphism manifests as various distinct morphotypes ranging from punctiform, bicentric, and ring-shaped to larger coiled forms, indicating that the B chromosomes may undergo rapid structural changes. During meiosis, B chromosomes display transmission drive through asymmetric segregation, preferentially accumulating in one daughter cell. Most B chromosomes formed univalents, with few forming bivalents or trivalents at meiosis I. Three unconventional retention mechanisms were identified in univalent B chromosomes: (1) associating with a nonhomologous chromosome, (2) accumulating near spindle poles, and (3) contributing to unequal spindle formation. Based on the abundant chromosomal changes of A chromosomes and stable XX/XO sex determination, we infer that the B chromosomes likely originated from multiple A chromosomes in B. cirratus. The roles of B chromosomes in the cell cycle and individual fitness are briefly discussed, and the evolutionary scenario is putatively put forward for the diversification of B chromosomes.
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publishDate 2025-06-01
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series Comparative Cytogenetics
spelling doaj-art-b38fc862586d4a84bd4abfb3de0da17c2025-08-20T02:30:30ZengPensoft PublishersComparative Cytogenetics1993-078X2025-06-01199110710.3897/compcytogen.19.153340153340Dynamic meiotic behavior and evolutionary insights of supernumerary B chromosomes in the hangingfly Bittacus cirratus (Mecoptera, Bittacidae)Le-Le He0Ying Miao1He-Hong Wang2Jie Zhang3Bao-Zhen Hua4Northwest A&F UniversityNingxia UniversityNorthwest A&F UniversityNorthwest A&F UniversityNorthwest A&F UniversitySupernumerary B chromosomes are significant dispensable genetic elements that follow their own species-specific evolutionary pathways. Despite their widespread occurrence, comprehensive analyses of their meiotic behavior remain limited. In this study, we present the first systematic investigation of B chromosome morphology and meiotic behavior in the hangingfly Bittacus cirratus Tjeder, 1956 using cytogenetic approaches. The male basal chromosome numbers of B. cirratus is 2n = 30 + XO, with 0–5 polymorphic B chromosomes. Intraspecific B chromosome polymorphism manifests as various distinct morphotypes ranging from punctiform, bicentric, and ring-shaped to larger coiled forms, indicating that the B chromosomes may undergo rapid structural changes. During meiosis, B chromosomes display transmission drive through asymmetric segregation, preferentially accumulating in one daughter cell. Most B chromosomes formed univalents, with few forming bivalents or trivalents at meiosis I. Three unconventional retention mechanisms were identified in univalent B chromosomes: (1) associating with a nonhomologous chromosome, (2) accumulating near spindle poles, and (3) contributing to unequal spindle formation. Based on the abundant chromosomal changes of A chromosomes and stable XX/XO sex determination, we infer that the B chromosomes likely originated from multiple A chromosomes in B. cirratus. The roles of B chromosomes in the cell cycle and individual fitness are briefly discussed, and the evolutionary scenario is putatively put forward for the diversification of B chromosomes.https://compcytogen.pensoft.net/article/153340/download/pdf/
spellingShingle Le-Le He
Ying Miao
He-Hong Wang
Jie Zhang
Bao-Zhen Hua
Dynamic meiotic behavior and evolutionary insights of supernumerary B chromosomes in the hangingfly Bittacus cirratus (Mecoptera, Bittacidae)
Comparative Cytogenetics
title Dynamic meiotic behavior and evolutionary insights of supernumerary B chromosomes in the hangingfly Bittacus cirratus (Mecoptera, Bittacidae)
title_full Dynamic meiotic behavior and evolutionary insights of supernumerary B chromosomes in the hangingfly Bittacus cirratus (Mecoptera, Bittacidae)
title_fullStr Dynamic meiotic behavior and evolutionary insights of supernumerary B chromosomes in the hangingfly Bittacus cirratus (Mecoptera, Bittacidae)
title_full_unstemmed Dynamic meiotic behavior and evolutionary insights of supernumerary B chromosomes in the hangingfly Bittacus cirratus (Mecoptera, Bittacidae)
title_short Dynamic meiotic behavior and evolutionary insights of supernumerary B chromosomes in the hangingfly Bittacus cirratus (Mecoptera, Bittacidae)
title_sort dynamic meiotic behavior and evolutionary insights of supernumerary b chromosomes in the hangingfly bittacus cirratus mecoptera bittacidae
url https://compcytogen.pensoft.net/article/153340/download/pdf/
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