Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA Sequencing
OBJECTIVE: The primary objective of our study was to compare the endometrial microbiota profiles of women with unexplained infertility and fertile women. STUDY DESIGN: In this case control study a total of 15 patients with unexplained infertility and 15 patients who had a live birth in the last t...
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| Format: | Article |
| Language: | English |
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Medical Network
2023-12-01
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| Series: | Gynecology Obstetrics & Reproductive Medicine |
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| Online Access: | https://gorm.com.tr/index.php/GORM/article/view/1452 |
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| author | Gonca Ozten Dere Cagman Tan Sezcan Mumusoglu Pinar Zarakolu Gurkan Bozdag |
| author_facet | Gonca Ozten Dere Cagman Tan Sezcan Mumusoglu Pinar Zarakolu Gurkan Bozdag |
| author_sort | Gonca Ozten Dere |
| collection | DOAJ |
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OBJECTIVE: The primary objective of our study was to compare the endometrial microbiota profiles of women with unexplained infertility and fertile women.
STUDY DESIGN: In this case control study a total of 15 patients with unexplained infertility and 15 patients who had a live birth in the last two years with proven fertility were recruited. Endometrial samples were collected and analyzed through next-generation sequencing of the bacteria-specific 16S ribosome gene.
RESULTS: Lactobacillus species represented the majority of the microbiome profile in both groups. The median percentage of the endometrial Lactobacillus between infertile patients and fertile patients was not statistically significant (p=0.9). Groups were divided into two categories: Lactobasillus dominant (Lactobacillus spp.> 90%, LD) and Non-Lactobacillus dominant (NLD). Lactobacillus dominance was detected in six of nine patients (66.7%) in the study group and four of nine patients (44.5%) in the control group, and no significant difference was observed between them (p=0.64).
CONCLUSION: According to our study results, no significant difference was observed between the endometrial microbiota profile of infertile and fertile patients. Further studies with larger sample sizes are needed to characterize the endometrial microbiota and its impact on reproduction.
Keywords: Microbiota, unexplained infertility, endometrial microbiota
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| format | Article |
| id | doaj-art-b363813a346c4efc8212ff1e54b14d4f |
| institution | Kabale University |
| issn | 1300-4751 2602-4918 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Medical Network |
| record_format | Article |
| series | Gynecology Obstetrics & Reproductive Medicine |
| spelling | doaj-art-b363813a346c4efc8212ff1e54b14d4f2025-02-11T21:17:48ZengMedical NetworkGynecology Obstetrics & Reproductive Medicine1300-47512602-49182023-12-0129310.21613/GORM.2023.1452Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA SequencingGonca Ozten Dere0Cagman Tan1Sezcan Mumusoglu2Pinar Zarakolu3Gurkan Bozdag4tobb etu university hospitalErciyes UniversityHacettepe UniversityHacettepe UniversityBahçeçi Health Group OBJECTIVE: The primary objective of our study was to compare the endometrial microbiota profiles of women with unexplained infertility and fertile women. STUDY DESIGN: In this case control study a total of 15 patients with unexplained infertility and 15 patients who had a live birth in the last two years with proven fertility were recruited. Endometrial samples were collected and analyzed through next-generation sequencing of the bacteria-specific 16S ribosome gene. RESULTS: Lactobacillus species represented the majority of the microbiome profile in both groups. The median percentage of the endometrial Lactobacillus between infertile patients and fertile patients was not statistically significant (p=0.9). Groups were divided into two categories: Lactobasillus dominant (Lactobacillus spp.> 90%, LD) and Non-Lactobacillus dominant (NLD). Lactobacillus dominance was detected in six of nine patients (66.7%) in the study group and four of nine patients (44.5%) in the control group, and no significant difference was observed between them (p=0.64). CONCLUSION: According to our study results, no significant difference was observed between the endometrial microbiota profile of infertile and fertile patients. Further studies with larger sample sizes are needed to characterize the endometrial microbiota and its impact on reproduction. Keywords: Microbiota, unexplained infertility, endometrial microbiota https://gorm.com.tr/index.php/GORM/article/view/1452Endometrial microbiotaMicrobiotaUnexplained infertilit |
| spellingShingle | Gonca Ozten Dere Cagman Tan Sezcan Mumusoglu Pinar Zarakolu Gurkan Bozdag Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA Sequencing Gynecology Obstetrics & Reproductive Medicine Endometrial microbiota Microbiota Unexplained infertilit |
| title | Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA Sequencing |
| title_full | Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA Sequencing |
| title_fullStr | Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA Sequencing |
| title_full_unstemmed | Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA Sequencing |
| title_short | Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA Sequencing |
| title_sort | comparison of endometrial microbiota results between patients with unexplained infertility and fertile patients through 16s rrna sequencing |
| topic | Endometrial microbiota Microbiota Unexplained infertilit |
| url | https://gorm.com.tr/index.php/GORM/article/view/1452 |
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