Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy
ObjectivesThis study aimed to introduce the use of laser-induced breakdown spectroscopy (LIBS) for evaluation of the mineral content of root canal dentin, and to assess whether a correlation exists between LIBS and scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) methods by comp...
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Korean Academy of Conservative Dentistry
2018-02-01
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| Series: | Restorative Dentistry & Endodontics |
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| Online Access: | http://rde.ac/upload/pdf/rde-43-e11.pdf |
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| author | Selen Küçükkaya Eren Emel Uzunoğlu Banu Sezer Zeliha Yılmaz İsmail Hakkı Boyacı |
| author_facet | Selen Küçükkaya Eren Emel Uzunoğlu Banu Sezer Zeliha Yılmaz İsmail Hakkı Boyacı |
| author_sort | Selen Küçükkaya Eren |
| collection | DOAJ |
| description | ObjectivesThis study aimed to introduce the use of laser-induced breakdown spectroscopy (LIBS) for evaluation of the mineral content of root canal dentin, and to assess whether a correlation exists between LIBS and scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) methods by comparing the effects of irrigation solutions on the mineral content change of root canal dentin.Materials and MethodsForty teeth with a single root canal were decoronated and longitudinally sectioned to expose the canals. The root halves were divided into 4 groups (n = 10) according to the solution applied: group NaOCl, 5.25% sodium hypochlorite (NaOCl) for 1 hour; group EDTA, 17% ethylenediaminetetraacetic acid (EDTA) for 2 minutes; group NaOCl+EDTA, 5.25% NaOCl for 1 hour and 17% EDTA for 2 minutes; a control group. Each root half belonging to the same root was evaluated for mineral content with either LIBS or SEM/EDS methods. The data were analyzed statistically.ResultsIn groups NaOCl and NaOCl+EDTA, the calcium (Ca)/phosphorus (P) ratio decreased while the sodium (Na) level increased compared with the other groups (p < 0.05). The magnesium (Mg) level changes were not significant among the groups. A significant positive correlation was found between the results of LIBS and SEM/EDS analyses (r = 0.84, p < 0.001).ConclusionsTreatment with NaOCl for 1 hour altered the mineral content of dentin, while EDTA application for 2 minutes had no effect on the elemental composition. The LIBS method proved to be reliable while providing data for the elemental composition of root canal dentin. |
| format | Article |
| id | doaj-art-b3583a4554864e68bbadea5ce01778c1 |
| institution | OA Journals |
| issn | 2234-7658 2234-7666 |
| language | English |
| publishDate | 2018-02-01 |
| publisher | Korean Academy of Conservative Dentistry |
| record_format | Article |
| series | Restorative Dentistry & Endodontics |
| spelling | doaj-art-b3583a4554864e68bbadea5ce01778c12025-08-20T02:38:30ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662018-02-01431e1110.5395/rde.2018.43.e11802Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopySelen Küçükkaya Eren0Emel Uzunoğlu1Banu Sezer2Zeliha Yılmaz3İsmail Hakkı Boyacı4Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, TurkeyDepartment of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, TurkeyDepartment of Food Engineering, Faculty of Engineering, Hacettepe University, Ankara, TurkeyDepartment of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, TurkeyDepartment of Food Engineering, Faculty of Engineering, Hacettepe University, Ankara, TurkeyObjectivesThis study aimed to introduce the use of laser-induced breakdown spectroscopy (LIBS) for evaluation of the mineral content of root canal dentin, and to assess whether a correlation exists between LIBS and scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) methods by comparing the effects of irrigation solutions on the mineral content change of root canal dentin.Materials and MethodsForty teeth with a single root canal were decoronated and longitudinally sectioned to expose the canals. The root halves were divided into 4 groups (n = 10) according to the solution applied: group NaOCl, 5.25% sodium hypochlorite (NaOCl) for 1 hour; group EDTA, 17% ethylenediaminetetraacetic acid (EDTA) for 2 minutes; group NaOCl+EDTA, 5.25% NaOCl for 1 hour and 17% EDTA for 2 minutes; a control group. Each root half belonging to the same root was evaluated for mineral content with either LIBS or SEM/EDS methods. The data were analyzed statistically.ResultsIn groups NaOCl and NaOCl+EDTA, the calcium (Ca)/phosphorus (P) ratio decreased while the sodium (Na) level increased compared with the other groups (p < 0.05). The magnesium (Mg) level changes were not significant among the groups. A significant positive correlation was found between the results of LIBS and SEM/EDS analyses (r = 0.84, p < 0.001).ConclusionsTreatment with NaOCl for 1 hour altered the mineral content of dentin, while EDTA application for 2 minutes had no effect on the elemental composition. The LIBS method proved to be reliable while providing data for the elemental composition of root canal dentin.http://rde.ac/upload/pdf/rde-43-e11.pdfdentinendodonticsmicroscopyspectrum analysis |
| spellingShingle | Selen Küçükkaya Eren Emel Uzunoğlu Banu Sezer Zeliha Yılmaz İsmail Hakkı Boyacı Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy Restorative Dentistry & Endodontics dentin endodontics microscopy spectrum analysis |
| title | Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy |
| title_full | Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy |
| title_fullStr | Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy |
| title_full_unstemmed | Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy |
| title_short | Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy |
| title_sort | mineral content analysis of root canal dentin using laser induced breakdown spectroscopy |
| topic | dentin endodontics microscopy spectrum analysis |
| url | http://rde.ac/upload/pdf/rde-43-e11.pdf |
| work_keys_str_mv | AT selenkucukkayaeren mineralcontentanalysisofrootcanaldentinusinglaserinducedbreakdownspectroscopy AT emeluzunoglu mineralcontentanalysisofrootcanaldentinusinglaserinducedbreakdownspectroscopy AT banusezer mineralcontentanalysisofrootcanaldentinusinglaserinducedbreakdownspectroscopy AT zelihayılmaz mineralcontentanalysisofrootcanaldentinusinglaserinducedbreakdownspectroscopy AT ismailhakkıboyacı mineralcontentanalysisofrootcanaldentinusinglaserinducedbreakdownspectroscopy |