Regulating cleavage activity and enabling microRNA detection with split sgRNA in Cas12b

Regulating cleavage activity and enabling microRNA detection with split sgRNA in Cas12b

Abstract The CRISPR-Cas12b system has revolutionized molecular diagnostics, yet its reliance on single guide RNAs (sgRNAs) exceeding 100 nt limits precise regulation and applications. We present a split sgRNA strategy for Cas12b, utilizing universal components with customizable Spacer to detect vari...

Full description

Saved in:
Bibliographic Details
Main Authors: Jiaqi Wang, Xiaofang Ye, Yuanfang Liu, Wentao Li, Xue Zhang, Wei Zhang, Changqing Yi, Chaoxing Liu
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61748-4
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items

Similar Items