Detecting the antibacterial activity of green synthesized silver (Ag) nanoparticles functionalized with ampicillin (Amp)

In the current study, synthesis and characterization of silver nanoparticles (AgNPs) before and after functionalization with ampicillin antibiotic and their application as anti-pathogenic agents towards bacteria were investigated. AgNPs were synthesized by a green method from AgNO3 solution with glu...

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Main Author: Baghdad Science Journal
Format: Article
Language:English
Published: University of Baghdad, College of Science for Women 2017-03-01
Series:مجلة بغداد للعلوم
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Online Access:http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2345
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author Baghdad Science Journal
author_facet Baghdad Science Journal
author_sort Baghdad Science Journal
collection DOAJ
description In the current study, synthesis and characterization of silver nanoparticles (AgNPs) before and after functionalization with ampicillin antibiotic and their application as anti-pathogenic agents towards bacteria were investigated. AgNPs were synthesized by a green method from AgNO3 solution with glucose subjected to microwave radiation. Characterization of the nanoparticles was conducted using UV-Vis spectroscopy, scanning electron microscopy (SEM), zeta potential determination and Fourier transform infrared (FTIR) spectroscopy. From SEM analysis, the typical silver nanoparticle particle size was found to be 30 nm and Zeta potential measurements gave information about particle stability. Analysis of FTIR patterns and UV-VIS spectroscopy confirmed the production of nanosilver particles. The activity of produced silver NP was tested against three pathogens (Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii) in both liquid and solid growth medium. AgNPs presented potential antibacterial activity, against tested bacteria. Ag and Ag-AMP nanoparticles were detected to have penitent antimicrobial. The optical density (OD) of the culture solution and measuring zones of inhibition were used to monitor the growth of bacteria in liquid and solid growth medium respectively
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issn 2078-8665
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publishDate 2017-03-01
publisher University of Baghdad, College of Science for Women
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series مجلة بغداد للعلوم
spelling doaj-art-b3552277dfb84ed6873dc740e25c2ead2025-08-20T03:57:47ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862017-03-0114110.21123/bsj.14.1.117-125Detecting the antibacterial activity of green synthesized silver (Ag) nanoparticles functionalized with ampicillin (Amp)Baghdad Science JournalIn the current study, synthesis and characterization of silver nanoparticles (AgNPs) before and after functionalization with ampicillin antibiotic and their application as anti-pathogenic agents towards bacteria were investigated. AgNPs were synthesized by a green method from AgNO3 solution with glucose subjected to microwave radiation. Characterization of the nanoparticles was conducted using UV-Vis spectroscopy, scanning electron microscopy (SEM), zeta potential determination and Fourier transform infrared (FTIR) spectroscopy. From SEM analysis, the typical silver nanoparticle particle size was found to be 30 nm and Zeta potential measurements gave information about particle stability. Analysis of FTIR patterns and UV-VIS spectroscopy confirmed the production of nanosilver particles. The activity of produced silver NP was tested against three pathogens (Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii) in both liquid and solid growth medium. AgNPs presented potential antibacterial activity, against tested bacteria. Ag and Ag-AMP nanoparticles were detected to have penitent antimicrobial. The optical density (OD) of the culture solution and measuring zones of inhibition were used to monitor the growth of bacteria in liquid and solid growth medium respectivelyhttp://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2345Silver nanoparticles, biosynthesis, antibacterial, glucose, microwave
spellingShingle Baghdad Science Journal
Detecting the antibacterial activity of green synthesized silver (Ag) nanoparticles functionalized with ampicillin (Amp)
مجلة بغداد للعلوم
Silver nanoparticles, biosynthesis, antibacterial, glucose, microwave
title Detecting the antibacterial activity of green synthesized silver (Ag) nanoparticles functionalized with ampicillin (Amp)
title_full Detecting the antibacterial activity of green synthesized silver (Ag) nanoparticles functionalized with ampicillin (Amp)
title_fullStr Detecting the antibacterial activity of green synthesized silver (Ag) nanoparticles functionalized with ampicillin (Amp)
title_full_unstemmed Detecting the antibacterial activity of green synthesized silver (Ag) nanoparticles functionalized with ampicillin (Amp)
title_short Detecting the antibacterial activity of green synthesized silver (Ag) nanoparticles functionalized with ampicillin (Amp)
title_sort detecting the antibacterial activity of green synthesized silver ag nanoparticles functionalized with ampicillin amp
topic Silver nanoparticles, biosynthesis, antibacterial, glucose, microwave
url http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2345
work_keys_str_mv AT baghdadsciencejournal detectingtheantibacterialactivityofgreensynthesizedsilveragnanoparticlesfunctionalizedwithampicillinamp