Ni<sup>2+</sup> and Cd<sup>2+</sup> Biosorption Capacity and Redox-Mediated Toxicity Reduction in Bacterial Strains from Highly Contaminated Soils of Uzbekistan
In this study, Ni<sup>2+</sup> and Cd<sup>2+</sup> resistant <i>Pseudomonas aeruginosa</i> 18, <i>Enterobacter ludwigii</i> 11Uz, and <i>Enterobacter cloacae</i> Uz_5 strains were isolated from soils contaminated with heavy metals in the Sa...
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2025-06-01
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| author | Aziza Usmonkulova Eligio Malusa Gulchekhra Kadirova Ilkhom Khalilov Loredana Canfora Liliya Abdulmyanova |
| author_facet | Aziza Usmonkulova Eligio Malusa Gulchekhra Kadirova Ilkhom Khalilov Loredana Canfora Liliya Abdulmyanova |
| author_sort | Aziza Usmonkulova |
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| description | In this study, Ni<sup>2+</sup> and Cd<sup>2+</sup> resistant <i>Pseudomonas aeruginosa</i> 18, <i>Enterobacter ludwigii</i> 11Uz, and <i>Enterobacter cloacae</i> Uz_5 strains were isolated from soils contaminated with heavy metals in the Samarkand and Kashkadarya regions (Uzbekistan), and tested to remove Ni<sup>2+</sup> and Cd<sup>2+</sup> ions from the environment via biosorption. The biosorption capacity of these strains was observed under in vitro conditions. The biosorption process was highly dependent on the growing conditions, with the highest biosorption rate observed after 300 min of incubation at pH 7.0, and 40 °C. The presence of functional groups such as S=O, NH<sub>2</sub>, and COOH in the biosorbing microorganisms was confirmed by IR spectroscopy. The adsorption capacity decreased when the initial metal concentration was increased and was enhanced with higher microbial biomass. <i>Enterobacter ludwigii</i> 11Uz strain was found to alter the toxic oxidation state of Ni<sup>2+</sup> and Cd<sup>2+</sup> cations, while <i>Pseudomonas aeruginosa</i> 18 and <i>Enterobacter cloacae</i> Uz_5 strains reduced the toxicity of Ni<sup>2+</sup> cations only by changing their oxidation state. It was confirmed in our studies that the three selected bacterial strains actively participated in the detoxification of Cd<sup>2+</sup> through the synthesis of cysteine amino acid. |
| format | Article |
| id | doaj-art-b34ea3582bc14d1c8f7ba43364869f45 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-b34ea3582bc14d1c8f7ba43364869f452025-08-20T03:56:46ZengMDPI AGMicroorganisms2076-26072025-06-01137148510.3390/microorganisms13071485Ni<sup>2+</sup> and Cd<sup>2+</sup> Biosorption Capacity and Redox-Mediated Toxicity Reduction in Bacterial Strains from Highly Contaminated Soils of UzbekistanAziza Usmonkulova0Eligio Malusa1Gulchekhra Kadirova2Ilkhom Khalilov3Loredana Canfora4Liliya Abdulmyanova5Institute of Microbiology of Academy Sciences of the Republic of Uzbekistan, st. A. Kadyri 7B, 100128 Tashkent, UzbekistanCouncil for Agriculture Research and Economics, Research Center for Viticulture and Enology, via P. Micca 35, 14100 Asti, ItalyInstitute of Microbiology of Academy Sciences of the Republic of Uzbekistan, st. A. Kadyri 7B, 100128 Tashkent, UzbekistanInstitute of Microbiology of Academy Sciences of the Republic of Uzbekistan, st. A. Kadyri 7B, 100128 Tashkent, UzbekistanCouncil for Agriculture Research and Economics, Research Center for Agriculture and Environment, via della Navicella 2-4, 00184 Rome, ItalyInstitute of Microbiology of Academy Sciences of the Republic of Uzbekistan, st. A. Kadyri 7B, 100128 Tashkent, UzbekistanIn this study, Ni<sup>2+</sup> and Cd<sup>2+</sup> resistant <i>Pseudomonas aeruginosa</i> 18, <i>Enterobacter ludwigii</i> 11Uz, and <i>Enterobacter cloacae</i> Uz_5 strains were isolated from soils contaminated with heavy metals in the Samarkand and Kashkadarya regions (Uzbekistan), and tested to remove Ni<sup>2+</sup> and Cd<sup>2+</sup> ions from the environment via biosorption. The biosorption capacity of these strains was observed under in vitro conditions. The biosorption process was highly dependent on the growing conditions, with the highest biosorption rate observed after 300 min of incubation at pH 7.0, and 40 °C. The presence of functional groups such as S=O, NH<sub>2</sub>, and COOH in the biosorbing microorganisms was confirmed by IR spectroscopy. The adsorption capacity decreased when the initial metal concentration was increased and was enhanced with higher microbial biomass. <i>Enterobacter ludwigii</i> 11Uz strain was found to alter the toxic oxidation state of Ni<sup>2+</sup> and Cd<sup>2+</sup> cations, while <i>Pseudomonas aeruginosa</i> 18 and <i>Enterobacter cloacae</i> Uz_5 strains reduced the toxicity of Ni<sup>2+</sup> cations only by changing their oxidation state. It was confirmed in our studies that the three selected bacterial strains actively participated in the detoxification of Cd<sup>2+</sup> through the synthesis of cysteine amino acid.https://www.mdpi.com/2076-2607/13/7/1485nickelcadmiumoxidation statebiosorption capacityIR spectroscopyresistant bacteria |
| spellingShingle | Aziza Usmonkulova Eligio Malusa Gulchekhra Kadirova Ilkhom Khalilov Loredana Canfora Liliya Abdulmyanova Ni<sup>2+</sup> and Cd<sup>2+</sup> Biosorption Capacity and Redox-Mediated Toxicity Reduction in Bacterial Strains from Highly Contaminated Soils of Uzbekistan Microorganisms nickel cadmium oxidation state biosorption capacity IR spectroscopy resistant bacteria |
| title | Ni<sup>2+</sup> and Cd<sup>2+</sup> Biosorption Capacity and Redox-Mediated Toxicity Reduction in Bacterial Strains from Highly Contaminated Soils of Uzbekistan |
| title_full | Ni<sup>2+</sup> and Cd<sup>2+</sup> Biosorption Capacity and Redox-Mediated Toxicity Reduction in Bacterial Strains from Highly Contaminated Soils of Uzbekistan |
| title_fullStr | Ni<sup>2+</sup> and Cd<sup>2+</sup> Biosorption Capacity and Redox-Mediated Toxicity Reduction in Bacterial Strains from Highly Contaminated Soils of Uzbekistan |
| title_full_unstemmed | Ni<sup>2+</sup> and Cd<sup>2+</sup> Biosorption Capacity and Redox-Mediated Toxicity Reduction in Bacterial Strains from Highly Contaminated Soils of Uzbekistan |
| title_short | Ni<sup>2+</sup> and Cd<sup>2+</sup> Biosorption Capacity and Redox-Mediated Toxicity Reduction in Bacterial Strains from Highly Contaminated Soils of Uzbekistan |
| title_sort | ni sup 2 sup and cd sup 2 sup biosorption capacity and redox mediated toxicity reduction in bacterial strains from highly contaminated soils of uzbekistan |
| topic | nickel cadmium oxidation state biosorption capacity IR spectroscopy resistant bacteria |
| url | https://www.mdpi.com/2076-2607/13/7/1485 |
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