Innovations in radiotherapy for tongue squamous cell carcinoma
Abstract Radiotherapy sensitivity is associated with the prognosis of patients with tongue squamous cell carcinoma (TSCC). In the present study, we proposed to explore the specific mechanism of interventional radiology (IR) therapy for TSCC in vitro and in vivo. TSCC cells were treated with 6 Gy IR...
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BMC
2024-11-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-024-05885-2 |
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| author | Songling Hu Xiaofei Li Bin Yang Tian Yu Fangyu Yi Xiurong Qin Cong Chen Can Wang Xin Yu Jing Zhu |
| author_facet | Songling Hu Xiaofei Li Bin Yang Tian Yu Fangyu Yi Xiurong Qin Cong Chen Can Wang Xin Yu Jing Zhu |
| author_sort | Songling Hu |
| collection | DOAJ |
| description | Abstract Radiotherapy sensitivity is associated with the prognosis of patients with tongue squamous cell carcinoma (TSCC). In the present study, we proposed to explore the specific mechanism of interventional radiology (IR) therapy for TSCC in vitro and in vivo. TSCC cells were treated with 6 Gy IR and tumor bearing mice were treated with 20 Gy × 1 IR. DIA quantitative proteomics along with bioinformatics analysis were conducted in TSCC cells to investigate differential proteins related to IR and relation of which involved in TMEM147 and SPHK1 was confirmed by immunoprecipitation. Cell proliferation, apoptosis, autophagy& autophagy flux along with calcium signaling pathway detection were performed in vitro and in vivo. Our results showed that IR induced increasing calcium levels accompanied by up-regulated TMEM147 and down-regulated SPHK1 along with enhancing autophagy together with apoptosis. The effect of calcium overloading induced by IR on autophagy and apoptosis was dependent on increasing TMEM147 and decreasing SPHK1. However, IR-induced autophagy and apoptosis tended to be independent of only increasing calcium levels when down-regulating TMEM147 or up-regulating SPHK1 expression in vitro and in vivo. Our study suggested that calcium-mediated TMEM147/SPHK1 may promote autophagy and apoptosis to improve radiotherapy sensitivity in TSCC. |
| format | Article |
| id | doaj-art-b34d10ec4d8f4398bcf231daab84921e |
| institution | DOAJ |
| issn | 1479-5876 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-b34d10ec4d8f4398bcf231daab84921e2025-08-20T02:51:18ZengBMCJournal of Translational Medicine1479-58762024-11-0122111610.1186/s12967-024-05885-2Innovations in radiotherapy for tongue squamous cell carcinomaSongling Hu0Xiaofei Li1Bin Yang2Tian Yu3Fangyu Yi4Xiurong Qin5Cong Chen6Can Wang7Xin Yu8Jing Zhu9Department of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityAbstract Radiotherapy sensitivity is associated with the prognosis of patients with tongue squamous cell carcinoma (TSCC). In the present study, we proposed to explore the specific mechanism of interventional radiology (IR) therapy for TSCC in vitro and in vivo. TSCC cells were treated with 6 Gy IR and tumor bearing mice were treated with 20 Gy × 1 IR. DIA quantitative proteomics along with bioinformatics analysis were conducted in TSCC cells to investigate differential proteins related to IR and relation of which involved in TMEM147 and SPHK1 was confirmed by immunoprecipitation. Cell proliferation, apoptosis, autophagy& autophagy flux along with calcium signaling pathway detection were performed in vitro and in vivo. Our results showed that IR induced increasing calcium levels accompanied by up-regulated TMEM147 and down-regulated SPHK1 along with enhancing autophagy together with apoptosis. The effect of calcium overloading induced by IR on autophagy and apoptosis was dependent on increasing TMEM147 and decreasing SPHK1. However, IR-induced autophagy and apoptosis tended to be independent of only increasing calcium levels when down-regulating TMEM147 or up-regulating SPHK1 expression in vitro and in vivo. Our study suggested that calcium-mediated TMEM147/SPHK1 may promote autophagy and apoptosis to improve radiotherapy sensitivity in TSCC.https://doi.org/10.1186/s12967-024-05885-2Tongue squamous cell carcinomaInterventional radiologyCalciumTMEM147SPHK1Autophagy |
| spellingShingle | Songling Hu Xiaofei Li Bin Yang Tian Yu Fangyu Yi Xiurong Qin Cong Chen Can Wang Xin Yu Jing Zhu Innovations in radiotherapy for tongue squamous cell carcinoma Journal of Translational Medicine Tongue squamous cell carcinoma Interventional radiology Calcium TMEM147 SPHK1 Autophagy |
| title | Innovations in radiotherapy for tongue squamous cell carcinoma |
| title_full | Innovations in radiotherapy for tongue squamous cell carcinoma |
| title_fullStr | Innovations in radiotherapy for tongue squamous cell carcinoma |
| title_full_unstemmed | Innovations in radiotherapy for tongue squamous cell carcinoma |
| title_short | Innovations in radiotherapy for tongue squamous cell carcinoma |
| title_sort | innovations in radiotherapy for tongue squamous cell carcinoma |
| topic | Tongue squamous cell carcinoma Interventional radiology Calcium TMEM147 SPHK1 Autophagy |
| url | https://doi.org/10.1186/s12967-024-05885-2 |
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