Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells
Abstract Base editors are precise editing tools that employ deaminases to modify target DNA bases. The DYW-family of cytosine deaminases is structurally and phylogenetically distinct and might be harnessed for genome editing tools. We report a novel CRISPR/Cas9-cytosine base editor using SsdA, a DYW...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | Genome Biology |
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| Online Access: | https://doi.org/10.1186/s13059-025-03478-w |
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| author | Dingbo Zhang Fiona Parth Laura Matos da Silva Teng-Cheong Ha Axel Schambach Jens Boch |
| author_facet | Dingbo Zhang Fiona Parth Laura Matos da Silva Teng-Cheong Ha Axel Schambach Jens Boch |
| author_sort | Dingbo Zhang |
| collection | DOAJ |
| description | Abstract Base editors are precise editing tools that employ deaminases to modify target DNA bases. The DYW-family of cytosine deaminases is structurally and phylogenetically distinct and might be harnessed for genome editing tools. We report a novel CRISPR/Cas9-cytosine base editor using SsdA, a DYW-like deaminase and bacterial toxin. A G103S mutation in SsdA enhances C-to-T editing efficiency while reducing its toxicity. Truncations result in an extraordinarily small enzyme. The SsdA-base editor efficiently converts C-to-T in rice and barley protoplasts and induces mutations in rice plants and mammalian cells. The engineered SsdA is a highly efficient genome editing tool. |
| format | Article |
| id | doaj-art-b34992d188d2471c9f032ee0ca9fffec |
| institution | Kabale University |
| issn | 1474-760X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Genome Biology |
| spelling | doaj-art-b34992d188d2471c9f032ee0ca9fffec2025-02-09T12:39:22ZengBMCGenome Biology1474-760X2025-02-0126112110.1186/s13059-025-03478-wEngineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cellsDingbo Zhang0Fiona Parth1Laura Matos da Silva2Teng-Cheong Ha3Axel Schambach4Jens Boch5Institute of Plant Genetics, Leibniz Universität HannoverInstitute of Plant Genetics, Leibniz Universität HannoverInstitute of Plant Genetics, Leibniz Universität HannoverInstitute of Experimental Hematology, Hannover Medical SchoolInstitute of Experimental Hematology, Hannover Medical SchoolInstitute of Plant Genetics, Leibniz Universität HannoverAbstract Base editors are precise editing tools that employ deaminases to modify target DNA bases. The DYW-family of cytosine deaminases is structurally and phylogenetically distinct and might be harnessed for genome editing tools. We report a novel CRISPR/Cas9-cytosine base editor using SsdA, a DYW-like deaminase and bacterial toxin. A G103S mutation in SsdA enhances C-to-T editing efficiency while reducing its toxicity. Truncations result in an extraordinarily small enzyme. The SsdA-base editor efficiently converts C-to-T in rice and barley protoplasts and induces mutations in rice plants and mammalian cells. The engineered SsdA is a highly efficient genome editing tool.https://doi.org/10.1186/s13059-025-03478-wCrop improvementGene therapyBase editingCRISPR |
| spellingShingle | Dingbo Zhang Fiona Parth Laura Matos da Silva Teng-Cheong Ha Axel Schambach Jens Boch Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells Genome Biology Crop improvement Gene therapy Base editing CRISPR |
| title | Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells |
| title_full | Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells |
| title_fullStr | Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells |
| title_full_unstemmed | Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells |
| title_short | Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells |
| title_sort | engineering a bacterial toxin deaminase from the dyw family into a novel cytosine base editor for plants and mammalian cells |
| topic | Crop improvement Gene therapy Base editing CRISPR |
| url | https://doi.org/10.1186/s13059-025-03478-w |
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